Mcroscope PPx (animal lab procedures)
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Questions and Answers

Which microscope is commonly used in veterinary in-house laboratories

  • Compound (correct)
  • Electron
  • Dark field
  • Phase-contrast

    • Which microscope is used in research setting or large human medical facilities

  • Phase-contrast
  • Electron (correct)
  • Dark field
  • Compound light

    • Which microscope is used in reference laboratories

  • Dark field
  • Electron
  • Phase-contrast
  • Both A and C (correct)

    • What is the maximum optical tube length of the compound light microscope

    <p>160 mm</p> Signup and view all the answers

    Which of the following is the distance between the objective lens and the eyepiece

    <p>Optical tube length</p> Signup and view all the answers

    The total magnification of the compound light microscope is calculated by multiplying the ocular magnification by the objective magnification power. Identify the correct example

    <p>x10(ocular lens)*x40(objective lens)=x400 total magnification</p> Signup and view all the answers

    Which of the following solvents is used for care and maintenance of the compound light microscope

    <p>Both A and B</p> Signup and view all the answers

    [Compound light microscope] What can be used to remove excessive oil

    <p>Xylene</p> Signup and view all the answers

    Can you use oil on the slide when using the high-dry objective lens

    <p>No</p> Signup and view all the answers

    Can you use the coarse adjustment knob to focus on the specimen while the high-power lens is in place

    <p>No</p> Signup and view all the answers

    When to use course adjustment knob

    <p>Both B and C</p> Signup and view all the answers

    When using the x100 (oil-immersion) objective lens, what is the first step

    <p>Locate a suitable examination area using the ×10 (low- power) objective lens</p> Signup and view all the answers

    When using the x100 (oil-immersion) objective lens, what is the second step

    <p>Rotate the nosepiece to move the high-power objective lens into place and refocus on the area with the fine adjustment knob.</p> Signup and view all the answers

    Scanning

    <p>x4</p> Signup and view all the answers

    When using the x100 (oil-immersion) objective lens, what is the third step

    <p>Rotate the nosepiece so that it is halfway between the high-power and oil-immersion objective lenses</p> Signup and view all the answers

    When using the x100 (oil-immersion) objective lens, what is the fourth step

    <p>Place a drop of immersion oil on the slide.</p> Signup and view all the answers

    When using the x100 (oil immersion) objective lens, what is the fifth step

    <p>Rotate the nosepiece to bring the oil-immersion lens into place.</p> Signup and view all the answers

    When using the x100 (oil-immersion) objective lens, what is the sixth step

    <p>Use the fine adjustment knob to focus on the image.</p> Signup and view all the answers

    Low power

    <p>x10</p> Signup and view all the answers

    High-dry

    <p>x40</p> Signup and view all the answers

    Oil-immersion

    <p>x100</p> Signup and view all the answers

    Low oil-immersion

    <p>x50</p> Signup and view all the answers

    Supports both the oculars and the rotating nosepiece containing the objective lenses. Through a system of mirrors within, it projects the image from the objective lens to the eyepiece

    <p>Head (body tube)</p> Signup and view all the answers

    Vertical portion of the microscope connecting the base to the head.

    <p>Arm</p> Signup and view all the answers

    Used to make large adjustments in the distance between the specimen and the objective lens to focus on the specimen

    <p>Coarse adjustment knob:</p> Signup and view all the answers

    Used to make small or fine, adjustments in the distance between the specimen and the objective lens to focus on the specimen. It is used for precise focusing

    <p>Fine adjustment knob</p> Signup and view all the answers

    Contains a lens that focuses light on the specimen. It may have a height-adjustment knob that raises and lowers it (and the lens) to vary the light delivery. Generally, the best position for it is dose to the bottom surface of the stage.

    <p>Condenser</p> Signup and view all the answers

    This contains three or four objective lenses, which are changed by rotating the desired lens into the proper position over the specimen. It should be used to rotate the lens rather than grabbing the lenses themselves

    <p>Nosepiece</p> Signup and view all the answers

    As the arm, or lever, attached to the condenser is moved, which of the following opens and closes to permit a variable amount of light to pass through the condenser. It provides the best possible contrast for viewing a specimen

    <p>Iris diaphragm with lever</p> Signup and view all the answers

    Consists of two lenses that focus light from the light source on the object

    <p>Substage condenser</p> Signup and view all the answers

    Opens and closes to control the amount of light illuminating the object

    <p>Aperture diaphragm</p> Signup and view all the answers

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