Podcast
Questions and Answers
What is one of the factors to consider when stabilizing isolated proteins?
What is one of the factors to consider when stabilizing isolated proteins?
- Storing proteins at high temperatures
- Blocking degradative enzymes such as nucleases & proteases (correct)
- Reducing pH levels
- Increasing adsorption to surface
Which technique is NOT used in protein purification according to the text?
Which technique is NOT used in protein purification according to the text?
- Salting out
- Electrophoresis
- Chromatography
- Ionization (correct)
What happens when salt is added during the 'salting in' process of protein solubility?
What happens when salt is added during the 'salting in' process of protein solubility?
- Protein solubility increases as salt is added (correct)
- Additional ions intensify attractive forces between proteins
- Proteins precipitate at lower salt concentrations
- Solubility of protein decreases due to shielded charges
What is the most commonly used reagent for salting out proteins?
What is the most commonly used reagent for salting out proteins?
How are proteins fractionated according to solubility in the text?
How are proteins fractionated according to solubility in the text?
Which enzyme-linked assay technique is mentioned in the text for assaying proteins?
Which enzyme-linked assay technique is mentioned in the text for assaying proteins?
What happens to the solubility of a protein when its net charge is zero?
What happens to the solubility of a protein when its net charge is zero?
In chromatography, what is the purpose of the mobile phase?
In chromatography, what is the purpose of the mobile phase?
Which type of chromatography separates molecules based on size and shape?
Which type of chromatography separates molecules based on size and shape?
What type of matrix is frequently used in ion-exchange chromatography?
What type of matrix is frequently used in ion-exchange chromatography?
How are proteins eluted from an ion exchanger in chromatography?
How are proteins eluted from an ion exchanger in chromatography?
What is monitored in the column effluent during ion-exchange chromatography?
What is monitored in the column effluent during ion-exchange chromatography?
What is the purpose of affinity chromatography?
What is the purpose of affinity chromatography?
In affinity chromatography, how are proteins separated from other substances?
In affinity chromatography, how are proteins separated from other substances?
What is the role of pH in electrophoresis of proteins?
What is the role of pH in electrophoresis of proteins?
How are separated amino acids made visible in paper electrophoresis?
How are separated amino acids made visible in paper electrophoresis?
What is the purpose of SDS-PAGE in protein separation?
What is the purpose of SDS-PAGE in protein separation?
Why is Coomassie blue used in electrophoresis of proteins?
Why is Coomassie blue used in electrophoresis of proteins?
How does SDS bind to proteins?
How does SDS bind to proteins?
What property of proteins does SDS-PAGE estimate?
What property of proteins does SDS-PAGE estimate?
How are proteins separated in isoelectric focusing?
How are proteins separated in isoelectric focusing?
What forms the pH gradient in isoelectric focusing?
What forms the pH gradient in isoelectric focusing?
In protein sequencing, how can the number of different subunits be determined?
In protein sequencing, how can the number of different subunits be determined?
What does the equal amount of Glycine and Phenylalanine in insulin indicate?
What does the equal amount of Glycine and Phenylalanine in insulin indicate?
What is the key feature of using 1-fluoro-2,5-dinitrobenzene (FDNB) in protein sequencing for N-terminal identification?
What is the key feature of using 1-fluoro-2,5-dinitrobenzene (FDNB) in protein sequencing for N-terminal identification?
Which enzyme is typically used for C-terminal identification in protein sequencing?
Which enzyme is typically used for C-terminal identification in protein sequencing?
What is the function of dansyl chloride in protein sequencing for N-terminal identification?
What is the function of dansyl chloride in protein sequencing for N-terminal identification?
Which statement about carboxypeptidase A is correct?
Which statement about carboxypeptidase A is correct?
How does aminopeptidase contribute to protein sequencing?
How does aminopeptidase contribute to protein sequencing?
What caution is provided regarding carboxypeptidase hydrolysis in protein sequencing?
What caution is provided regarding carboxypeptidase hydrolysis in protein sequencing?
Flashcards are hidden until you start studying
