5 Questions
What is the purpose of western blotting or protein immunoblotting?
To identify a specific protein in a complex mixture.
What are the membranes used for the immobilization of proteins in western blotting?
Nitrocellulose or PVDF membranes.
Describe the process of transferring proteins from gel to the membrane in western blotting.
The transfer of proteins from the gel to the membrane is done electrophoretically.
How is the detection of transferred protein done in western blotting?
Using specific primary antibody and secondary enzyme labeled antibody and substrate.
What principle does western blotting utilize for the identification of specific antigens?
The principle of antigen-antibody interaction using monoclonal or polyclonal antibodies.
Test your knowledge of Western blotting techniques with this quiz from Irdava College of Engineering and Technology. Explore the principles and procedures involved in protein immunoblotting and the use of SDS-PAGE for protein separation. Ideal for biology and biochemistry enthusiasts.
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