Questions and Answers
What is the purpose of western blotting or protein immunoblotting?
To identify a specific protein in a complex mixture.
What are the membranes used for the immobilization of proteins in western blotting?
Nitrocellulose or PVDF membranes.
Describe the process of transferring proteins from gel to the membrane in western blotting.
The transfer of proteins from the gel to the membrane is done electrophoretically.
How is the detection of transferred protein done in western blotting?
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What principle does western blotting utilize for the identification of specific antigens?
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