Podcast
Questions and Answers
______ is a hybridization technique by which a macromolecule such as DNA, RNA, or protein is resolved in a gel matrix, transferred to a solid support, and detected with a specific probe
______ is a hybridization technique by which a macromolecule such as DNA, RNA, or protein is resolved in a gel matrix, transferred to a solid support, and detected with a specific probe
Blotting
______ (1981) is an Immunoblotting technique which rely on the specificity of binding between a protein of interest and a probe (antibody raised against that particular protein) to allow detection of the protein (cell, tissue, organ, or body fluid) of interest in a mixture of many other similar molecules
______ (1981) is an Immunoblotting technique which rely on the specificity of binding between a protein of interest and a probe (antibody raised against that particular protein) to allow detection of the protein (cell, tissue, organ, or body fluid) of interest in a mixture of many other similar molecules
Western blotting
Sample preparation All sources of protein, from whole tissues, biological fluids and ______ secreted in vitro, are open to analysis by Western blotting. Assorted detergents, salts, and chemical lysis buffers such as Tris-HCI to disrupt the cellular membranes and solubilize the target protei
Sample preparation All sources of protein, from whole tissues, biological fluids and ______ secreted in vitro, are open to analysis by Western blotting. Assorted detergents, salts, and chemical lysis buffers such as Tris-HCI to disrupt the cellular membranes and solubilize the target protei
proteins
______ 3. Blotting (or transfer) 4. Blocking 5. Antibody Probing 6. Detection 1. Sample preparation
______ 3. Blotting (or transfer) 4. Blocking 5. Antibody Probing 6. Detection 1. Sample preparation
Flashcards
Blotting
Blotting
A technique to resolve macromolecules like DNA, RNA, or proteins in a gel and detect them with a probe.
Western Blotting
Western Blotting
An immunoblotting technique that detects specific proteins using antibodies.
Sample Preparation
Sample Preparation
The initial step in Western blotting involving the extraction of proteins from tissues or fluids.
Gel Electrophoresis
Gel Electrophoresis
Signup and view all the flashcards
Detection
Detection
Signup and view all the flashcards
Study Notes
Western Blotting Technique
- Western blotting is a hybridization technique used to resolve and detect macromolecules like DNA, RNA, or proteins in a gel matrix, transferred to a solid support.
Immunoblotting
- Developed in 1981, Immunoblotting is a Western blotting technique that relies on the specificity of binding between a protein of interest and a probe (antibody) to detect the protein in a mixture of similar molecules.
Sample Preparation for Western Blotting
- Various sources of protein can be analyzed using Western blotting, including whole tissues, biological fluids, and secreted proteins in vitro.
- Sample preparation involves disrupting cellular membranes and solubilizing target proteins using detergents, salts, and chemical lysis buffers like Tris-HCI.
Western Blotting Protocol
- The Western blotting protocol consists of six steps:
- Sample preparation
- Blotting (or transfer)
- Blocking
- Antibody probing
- Detection
Studying That Suits You
Use AI to generate personalized quizzes and flashcards to suit your learning preferences.
