Questions and Answers
What can be a physiological condition that affects WBC count?
Which factor might lead to an erroneous WBC count?
What is a pathological condition that could result in an elevated WBC count?
What could cause a decrease in WBC count?
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Which of the following is NOT a factor affecting WBC count?
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What is the depth of the hemocytometer used for manual WBC counts?
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What shape is the moat in the hemocytometer designed with?
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How many square ruled areas are present in a hemocytometer?
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Which of the following best describes the purpose of a hemocytometer?
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In the context of the hemocytometer, what does 'WBC' stand for?
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What is the duration for mixing in the procedure described?
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What type of count is mentioned in the procedure?
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Which of the following is NOT mentioned in the procedure?
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What is the relevance of the name 'A.Kewan' in the context provided?
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What could be inferred if 'Mix for 2-3 minutes' is not followed?
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What is the age of A. Kewan?
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What type of medical procedure is mentioned in relation to A. Kewan?
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What does WBC stand for in the context of the count mentioned?
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Which of the following is NOT typically involved in a manual WBC count?
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What is the significance of performing a manual WBC count?
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Which of the following is categorized as a metabolic disorder?
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What type of condition could result from acute hemorrhage?
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Which of the following is NOT a type of poisoning?
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In the context of metabolic disorders, what is typically affected?
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What is a common consequence of both chemical and drug poisoning?
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Study Notes
Physiological and Pathological Conditions of WBC Count Variation
- Normal white blood cell (WBC) count ranges from 4,000 to 11,000 cells per microliter of blood.
- High WBC count (leukocytosis) can indicate infections, inflammation, stress, or allergic reactions.
- Low WBC count (leukopenia) may result from bone marrow disorders, autoimmune diseases, or viral infections.
- Metabolic disorders can contribute to abnormal WBC levels, impacting immune function.
- Drug poisoning and certain chemicals can lead to a decrease in WBC production or increase destruction.
- Acute hemorrhage can trigger WBC count alterations due to stress responses and the mobilization of immune cells.
Factors Causing Errors in WBC Count
- Inaccurate mixing of blood sample can lead to uneven distribution of leukocytes.
- Residue in the hemocytometer from previous samples may contaminate results.
- Improper counting techniques, such as miscounting cells in the ruled areas.
- Air bubbles in the counting chamber can obstruct visibility and lead to incorrect readings.
- Clumping of cells due to improper dilution or storage conditions can skew results.
- Timing issues, such as delays in analysis after blood collection, may alter WBC viability.
Properties of the Hemocytometer
- The hemocytometer has two square, ruled areas separated by an H-shaped moat for counting cells.
- Each ruled area enables precise cell counting over a designated volume due to its fixed depth of 0.1 mm.
- Proper sample preparation involves mixing for 2-3 minutes for accurate representation of WBCs.
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Description
This quiz focuses on understanding the physiological and pathological conditions that lead to variations in white blood cell counts. Additionally, it explores the various factors that can contribute to errors in WBC counting. Test your knowledge on this critical aspect of hematology.
