15 Questions
Which type of white blood cells is responsible for killing bacteria and fungi?
Neutrophils
What is the function of lymphocytes in the immune system?
Producing antibodies
Which staining method is used to differentiate various types of white blood cells on a glass slide?
Geimsa stain
What material is used as a spreader during the preparation of the blood smear on the glass slide?
2nd glass slide
How are the values of white blood cell types reported in a differential count?
By absolute number of each cell type
Which type of white blood cells is involved in allergic reactions and defense against parasites?
Basophils
What is the typical response to infection or serious injury?
Increased production of neutrophils
Which WBC type has cytoplasm full of granules and a nucleus with dense chromatin in 2-5 lobes?
Eosinophil
Which WBC type has a cytoplasm with dust-like lilac color granules and a large irregularly shaped and folded nucleus?
Monocyte
Which WBC type has a cytoplasm that is medium blue with a round, dense chromatin nucleus?
Lymphocyte
What is the normal percentage range of eosinophils in the differential count (DLC)?
1-4%
Which WBC type has cytoplasm that is pink and granules that are dark blue-black with an obscure nucleus?
Basophil
What does the suffix 'penia' typically indicate in a particular leukocyte?
Decreased cell count
What is the primary defense against infection?
Neutrophils
What is the typical appearance of the nucleus of a neutrophil?
Blue, dense chromatin with 2 lobes
Study Notes
White Blood Cells
- Neutrophils are responsible for killing bacteria and fungi
- Lymphocytes play a crucial role in the immune system by producing antibodies to fight infections and diseases
Blood Smear Preparation
- Romanowsky staining method is used to differentiate various types of white blood cells on a glass slide
- A spreader is used to prepare the blood smear on the glass slide, typically made of glass or plastic
Differential Count
- The values of white blood cell types are reported as a percentage of the total white blood cell count
- Differential count helps to identify the types of white blood cells and their relative proportions
White Blood Cell Types
- Eosinophils are involved in allergic reactions and defense against parasites
- Basophils have cytoplasm full of granules and a nucleus with dense chromatin in 2-5 lobes
- Lymphocytes have a cytoplasm with a round, dense chromatin nucleus and medium blue color
- Monocytes have a cytoplasm with dust-like lilac color granules and a large irregularly shaped and folded nucleus
- Eosinophils have cytoplasm that is pink and granules that are dark blue-black with an obscure nucleus
Normal Ranges
- The normal percentage range of eosinophils in the differential count is 1-3%
Leukocyte Terminology
- The suffix 'penia' typically indicates a decrease in a particular leukocyte
Immune Response
- The primary defense against infection is the production of white blood cells
- The typical response to infection or serious injury is the activation of white blood cells to fight against the infection
Neutrophil Morphology
- The typical appearance of the nucleus of a neutrophil is a nucleus with dense chromatin in 2-5 lobes
This quiz covers the differential leukocyte (white blood cell) count, which determines the number or percentage of each type of white blood cell present in the blood. It involves techniques such as smearing a drop of blood on a glass slide and staining it with a special dye for differentiation.
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