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Questions and Answers
What do the terms Km and V maxrepresent in enzyme kinetics?
What do the terms Km and V maxrepresent in enzyme kinetics?
- Constants for enzyme stability
- Parameters for reaction equilibrium
- Michaelis constant and maximum reaction velocity (correct)
- Rates of substrate binding and release
How can Km and V max be estimated using the Lineweaver-Burk plot?
How can Km and V max be estimated using the Lineweaver-Burk plot?
- By taking the reciprocals of the Michaelis-Menten equation (correct)
- By observing enzyme stability at different temperatures
- By measuring the final product concentration
- By plotting enzyme concentration against substrate concentration
What type of curve does the Michaelis-Menten equation produce when plotted?
What type of curve does the Michaelis-Menten equation produce when plotted?
- Linear
- Rectangular hyperbola (correct)
- Exponential
- Parabolic
What is a major drawback of using the Lineweaver-Burk plot?
What is a major drawback of using the Lineweaver-Burk plot?
How does increasing temperature typically affect enzyme activity?
How does increasing temperature typically affect enzyme activity?
What is the effect of pH on enzyme activity?
What is the effect of pH on enzyme activity?
What type of inhibitor is penicillin, and how does it affect bacterial enzymes?
What type of inhibitor is penicillin, and how does it affect bacterial enzymes?
Which of the following is a mechanism of irreversible enzyme inhibition?
Which of the following is a mechanism of irreversible enzyme inhibition?
What effect does a competitive inhibitor have on Km?
What effect does a competitive inhibitor have on Km?
In noncompetitive inhibition, how does the presence of the inhibitor affect Vmax?
In noncompetitive inhibition, how does the presence of the inhibitor affect Vmax?
What type of inhibitor mimics the substrate and competes for the active site?
What type of inhibitor mimics the substrate and competes for the active site?
What is the role of acetylcholinesterase in neurotransmission?
What is the role of acetylcholinesterase in neurotransmission?
Which nerve agent irreversibly inhibits acetylcholinesterase?
Which nerve agent irreversibly inhibits acetylcholinesterase?
What is the effect of a noncompetitive inhibitor on the enzyme-substrate complex?
What is the effect of a noncompetitive inhibitor on the enzyme-substrate complex?
What happens to enzyme activity when the temperature exceeds the optimal range?
What happens to enzyme activity when the temperature exceeds the optimal range?
What does the term "allosteric regulation" refer to?
What does the term "allosteric regulation" refer to?
What is the primary mechanism by which salicylic acid (aspirin) acts as a pain reliever?
What is the primary mechanism by which salicylic acid (aspirin) acts as a pain reliever?
In the Lineweaver-Burk plot, what do the x-intercept and y-intercept represent?
In the Lineweaver-Burk plot, what do the x-intercept and y-intercept represent?
How does temperature affect the kinetic parameters Km and Kcat?
How does temperature affect the kinetic parameters Km and Kcat?
Which of the following factors does NOT influence enzyme kinetics?
Which of the following factors does NOT influence enzyme kinetics?
What is the primary effect of irreversible inhibitors on enzyme function?
What is the primary effect of irreversible inhibitors on enzyme function?
What is the significance of measuring initial velocity v0 in enzyme kinetics?
What is the significance of measuring initial velocity v0 in enzyme kinetics?
Which type of inhibition is characterized by an inhibitor binding to an allosteric site?
Which type of inhibition is characterized by an inhibitor binding to an allosteric site?
Which of the following is a characteristic of competitive inhibitors?
Which of the following is a characteristic of competitive inhibitors?
What is the effect of a noncompetitive inhibitor on Km?
What is the effect of a noncompetitive inhibitor on Km?
What can be inferred from a high Km value?
What can be inferred from a high Km value?
What type of curve does a plot of 1/ v0 versus 1/[S] produce?
What type of curve does a plot of 1/ v0 versus 1/[S] produce?
Which of the following is a property of irreversible inhibitors?
Which of the following is a property of irreversible inhibitors?
What happens to enzyme activity at extreme pH levels?
What happens to enzyme activity at extreme pH levels?
Which of the following statements is true regarding enzyme kinetics?
Which of the following statements is true regarding enzyme kinetics?
Flashcards
Capital of France (example flashcard)
Capital of France (example flashcard)
Paris
