Virus Detection Plate Layout Instructions
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Questions and Answers

What is the purpose of adding a substrate in an ELISA test?

  • To change the color of the primary antibody
  • To increase the volume of the sample
  • To determine the patient's age
  • To convert the enzyme to a signal (correct)

In the presence of horse radish peroxidase, what color does ABTS turn?

  • Green (correct)
  • Blue
  • Yellow
  • Orange

What type of ELISA uses a direct conjugate of primary antibody?

  • Direct ELISA (correct)
  • Secondary Antibody Conjugate
  • Indirect ELISA
  • Adding Substrate ELISA

How can ELISA results be read and recorded?

<p>With a spectrophotometer (A)</p> Signup and view all the answers

What does the amount of color produced in ELISA indicate?

<p>The amount of primary antibody bound to the antigen (A)</p> Signup and view all the answers

Which type of ELISA uses a secondary antibody conjugate?

<p>Indirect ELISA (D)</p> Signup and view all the answers

What is the purpose of adding 200 µl of the substrate to the wells in columns (1), (2), and (4)?

<p>To test for the presence of alkaline phosphatase (C)</p> Signup and view all the answers

Which color indicates virus growth in the plate layout described?

<p>Yellow (C)</p> Signup and view all the answers

Based on the plate layout, what does a clear color indicate?

<p>No virus growth (A)</p> Signup and view all the answers

What is the significance of examining the plate for signs of growth and recording colonies observed?

<p>To identify the isolated organism (B)</p> Signup and view all the answers

Can Remdesivir be prescribed based on the described procedure?

<p>No, it has no impact on virus growth (B)</p> Signup and view all the answers

What is the type of hemolysis observed in this case?

<p>Alpha hemolysis (B)</p> Signup and view all the answers

Which laboratory technique will be assessed in the CW practical assessment?

<p>Gram staining (C)</p> Signup and view all the answers

What will happen if a student does not carry out a practical experiment as per the instructions?

<p>A note will be taken and the student will lose marks (B)</p> Signup and view all the answers

Which of the following symptoms were displayed by most of the infected abattoir workers?

<p>Slight breathlessness with headache (A)</p> Signup and view all the answers

What is used to assess practical skills in the CW practical assessment?

<p>Gram staining protocol (D)</p> Signup and view all the answers

Which of the following is an example of a laboratory technique mentioned in the text?

<p>Direct ELISA (B)</p> Signup and view all the answers

What will happen if a student skips a practical question in the CW practical assessment?

<p>The student's total mark will be reduced (A)</p> Signup and view all the answers

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