18 Questions
What is the purpose of adding a substrate in an ELISA test?
To convert the enzyme to a signal
In the presence of horse radish peroxidase, what color does ABTS turn?
Green
What type of ELISA uses a direct conjugate of primary antibody?
Direct ELISA
How can ELISA results be read and recorded?
With a spectrophotometer
What does the amount of color produced in ELISA indicate?
The amount of primary antibody bound to the antigen
Which type of ELISA uses a secondary antibody conjugate?
Indirect ELISA
What is the purpose of adding 200 µl of the substrate to the wells in columns (1), (2), and (4)?
To test for the presence of alkaline phosphatase
Which color indicates virus growth in the plate layout described?
Yellow
Based on the plate layout, what does a clear color indicate?
No virus growth
What is the significance of examining the plate for signs of growth and recording colonies observed?
To identify the isolated organism
Can Remdesivir be prescribed based on the described procedure?
No, it has no impact on virus growth
What is the type of hemolysis observed in this case?
Alpha hemolysis
Which laboratory technique will be assessed in the CW practical assessment?
Gram staining
What will happen if a student does not carry out a practical experiment as per the instructions?
A note will be taken and the student will lose marks
Which of the following symptoms were displayed by most of the infected abattoir workers?
Slight breathlessness with headache
What is used to assess practical skills in the CW practical assessment?
Gram staining protocol
Which of the following is an example of a laboratory technique mentioned in the text?
Direct ELISA
What will happen if a student skips a practical question in the CW practical assessment?
The student's total mark will be reduced
Follow the instructions to add substrate to specific wells in a plate layout for virus detection. Incubate the plates and record positive and negative wells based on colour development.
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