Virus Detection Plate Layout Instructions
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Questions and Answers

What is the purpose of adding a substrate in an ELISA test?

  • To change the color of the primary antibody
  • To increase the volume of the sample
  • To determine the patient's age
  • To convert the enzyme to a signal (correct)
  • In the presence of horse radish peroxidase, what color does ABTS turn?

  • Green (correct)
  • Blue
  • Yellow
  • Orange
  • What type of ELISA uses a direct conjugate of primary antibody?

  • Direct ELISA (correct)
  • Secondary Antibody Conjugate
  • Indirect ELISA
  • Adding Substrate ELISA
  • How can ELISA results be read and recorded?

    <p>With a spectrophotometer</p> Signup and view all the answers

    What does the amount of color produced in ELISA indicate?

    <p>The amount of primary antibody bound to the antigen</p> Signup and view all the answers

    Which type of ELISA uses a secondary antibody conjugate?

    <p>Indirect ELISA</p> Signup and view all the answers

    What is the purpose of adding 200 µl of the substrate to the wells in columns (1), (2), and (4)?

    <p>To test for the presence of alkaline phosphatase</p> Signup and view all the answers

    Which color indicates virus growth in the plate layout described?

    <p>Yellow</p> Signup and view all the answers

    Based on the plate layout, what does a clear color indicate?

    <p>No virus growth</p> Signup and view all the answers

    What is the significance of examining the plate for signs of growth and recording colonies observed?

    <p>To identify the isolated organism</p> Signup and view all the answers

    Can Remdesivir be prescribed based on the described procedure?

    <p>No, it has no impact on virus growth</p> Signup and view all the answers

    What is the type of hemolysis observed in this case?

    <p>Alpha hemolysis</p> Signup and view all the answers

    Which laboratory technique will be assessed in the CW practical assessment?

    <p>Gram staining</p> Signup and view all the answers

    What will happen if a student does not carry out a practical experiment as per the instructions?

    <p>A note will be taken and the student will lose marks</p> Signup and view all the answers

    Which of the following symptoms were displayed by most of the infected abattoir workers?

    <p>Slight breathlessness with headache</p> Signup and view all the answers

    What is used to assess practical skills in the CW practical assessment?

    <p>Gram staining protocol</p> Signup and view all the answers

    Which of the following is an example of a laboratory technique mentioned in the text?

    <p>Direct ELISA</p> Signup and view all the answers

    What will happen if a student skips a practical question in the CW practical assessment?

    <p>The student's total mark will be reduced</p> Signup and view all the answers

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