Veterinary Clinical Pathology - 4th Level

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Questions and Answers

What type of content appears to be repeatedly referenced within the provided links?

  • Instructions for system upgrades
  • A website for Windows 10 information (correct)
  • Documentation for software applications
  • Technical specifications for hardware components

Which of the following statements best describes the nature of the links provided?

  • They are likely resources for troubleshooting Windows 10. (correct)
  • They are links to social media platforms.
  • They consist of commercial advertisements.
  • They provide a wide variety of educational resources.

Given the repetitive nature of the links, what can be inferred about their intended purpose?

  • They may function as a guide for new users of Windows 10. (correct)
  • They are intended for user feedback collection.
  • They could be placeholders for future content updates.
  • They are likely meant for marketing Windows 10.

What is a possible misconception about the links provided within the content?

<p>They are official Microsoft resources. (B)</p> Signup and view all the answers

If the links are all pointing to the same site, what might this indicate about their reliability?

<p>They are likely to contain consistent information. (C)</p> Signup and view all the answers

Flashcards

URL

A unique address on the internet used to locate a specific resource, such as a webpage.

Internet Address

A numerical label assigned to each device connected to the internet.

Webpage Address

Specific address or location of a page on the world wide web.

Consistent URL

A URL that doesn't change.

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A specific internet address or URL pointing to a particular website or resource.

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Study Notes

Veterinary Clinical Pathology - 4th Level

  • Course Title: Clinical Pathology (General Program)
  • Instructor: Dr. Jihad Al-Saied
  • Section: Second Section

Atomic Absorption

  • Use: Measuring heavy metals
  • Principle: Atomization (dissociation from chemical bonds, placing in unexcited state). Each element absorbs characteristic wavelength (W.L.) in the ground state.
  • Process: Heavy metals separated from their chemical bonds then placed in the unexcited state.  Each element absorbs a unique wavelength.

Chromatography

  • Use: Separating mixtures into components for identification, purification, and quantification
  • Types:
    • Plane
    • Column
  • Column Types:
    • Liquid-liquid chromatography
    • Ion exchange chromatography
    • Steric (size) exclusion chromatography
    • HPLC
    • Gas chromatography

1- Liquid-liquid Chromatography

  • Separation: Based on solubility

2- Ion Exchange Chromatography

  • Separation: Based on anion exchange to separate unwanted substances

3- Steric (size) Exclusion Chromatography

  • Use (Sephadex): Smaller molecules diffuse into the gel column, while larger ones pass rapidly, appearing first in the fluid discharge. Smaller molecules will be delayed in the column.

4- HPLC

  • Method: Controlled pore size with pressure between 500-5000 pounds per square inch.

5- Gas Chromatography

  • Characteristics: Rapid, sensitive, accurate
  • Use: Separating and measuring nanogram and picogram amounts of volatile substances (steroids, lipids, drugs, blood alcohol, other toxic substances).

Electrochemistry

  • Method: Measures ions in solution by their electrical properties.
  • Techniques: Ion-selective electrodes (ISEs)
  • Selective membrane: Measures single ions (Na+, K+, Cl-, Ca2+, HCO3+)
  • Ion Contact with Membrane: Penetration changes potential measurements as voltage changes due to ionic activity.

Electrophoresis

  • Use: Fractionating serum proteins
  • Method: Migration of proteins in an electrical field
  • Factors: Direction and rate of migration based on protein charge and size
  • Buffer pH (8.6): Negative charge applied to serum proteins.

Electrophoresis - Molecules in Motion

  • Migration Direction: Molecules move towards the opposite charged electrode.
    • Anions (-ve charge) towards the positive (anode) electrode.
    • Cations (+ve charge) towards the negative (cathode) electrode.
  • Albumin: Strongest negative charge, migrates fastest toward the anode.
  • Globulin: Weaker negative charge, migrates slowly toward the anode.
  • Migration Pattern: Forms bands (use of media support).

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