VET 323 Veterinary Bacteriology & Mycology: Staphylococcus Genus Characteristics
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Questions and Answers

What test is used to distinguish Group A streptococci from other betahemolytic streptococci?

  • Bacitracin Susceptibility (correct)
  • Bile Solubility
  • CAMP Test
  • Optochin Susceptibility

Which test involves adding a suspension of suspected S. pneumonia to a solution of sodium taurocholate to observe autolysis?

  • Bile Solubility (correct)
  • CAMP Test
  • Bacitracin Susceptibility
  • Optochin Susceptibility

Which streptococcal group is identified through the CAMP Test and carotenoid pigmentation under anaerobic incubation?

  • S. pneumonia
  • Group B (S. agalactiae) (correct)
  • S. suis
  • Group A (S. pyogenes)

Which test involves the use of ethylhydrocupreine HCL to distinguish certain streptococci?

<p>Optochin Susceptibility (B)</p> Signup and view all the answers

What distinguishes Group A (S. pyogenes) from other betahemolytic streptococci?

<p>Bacitracin Susceptibility (B)</p> Signup and view all the answers

Which streptococcal species produces amylase rection?

<p>S. suis (A)</p> Signup and view all the answers

In which natural habitat do Enterococci occur?

<p>Soil, Food, Water, Plants (B)</p> Signup and view all the answers

What distinguishes Enterococcus as a genus from Streptococcus Group D?

<p>Natural Habitat (D)</p> Signup and view all the answers

In which strains of streptococci is the CAMP Test effective for identification?

<p>Both Group A and Group B (C)</p> Signup and view all the answers

What type of test is performed using a disc diffusion method involving ethylhydrocupreine HCL?

<p>Optochin Susceptibility (A)</p> Signup and view all the answers

Flashcards

Bacitracin Susceptibility

Differentiates Group A strep from other beta-hemolytic strep.

Bile Solubility

Test using sodium taurocholate to observe autolysis of suspected S. pneumoniae.

Group B Streptococcus

Identified by the CAMP Test and carotenoid pigmentation under anaerobic conditions.

Optochin Susceptibility

Test using ethylhydrocupreine HCL to distinguish certain strep.

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Bacitracin Susceptibility

Distinguishes Group A (S. pyogenes) from other beta-hemolytic strep.

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S. suis

A streptococcal species that produces amylase.

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Enterococci Habitat

Found in soil, food, water, and plants.

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Enterococcus vs. Strep D

Distinguished by its natural habitat.

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CAMP Test Effectiveness

Effective for identifying both Group A and Group B streptococci.

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Optochin Susceptibility Test

A disc diffusion test using ethylhydrocupreine HCL.

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Study Notes

Staphylococcus Species

  • Gram-positive cocci
  • Facultative anaerobes
  • Catalase positive, except for S. aureus subsp. anaerobius and S. saccharolyticus
  • Oxidase negative
  • Non-motile
  • Do not form spores
  • Presence of capsules, which may be lost in subculturing
  • Colonies are mucoid
  • Found on skin, skin glands, and mucous membranes
  • Trauma facilitates colonization of tissues

Morphology

  • Perfectly spherical, uniform in size (1 μm)
  • Irregular masses, resembling a bunch of grapes

Cultural Characteristics

  • Grow on blood agar (5%) with characteristic hemolysin production
  • Heavily contaminated material can be plated on Mannitol Salt Agar (MSA)
  • Inhibits Gram-negative bacteria
  • Resistant to salt, saline tolerant

Identification

  • Group C, G, and F
  • May be β-hemolytic
  • Associated with bacteremia, joint infections, pneumonia, and puerperal fever in humans

Genus Enterococcus

  • Formerly Streptococcus (Group D)
  • Genus Enterococcus was proposed in 1984
  • Found in soil, food, water, plants, animals, birds, and insects
  • Inhabit the gastrointestinal tract and female genital tract of humans and other animals

Characteristics of Enterococcus

  • Grow at 45°C and tolerate 40% bile
  • Hydrolyze aesculin

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Description

This quiz focuses on the characteristics of Staphylococcus species including their morphology, biochemical reactions, and presence of capsule. Test your knowledge about Gram positive, cocci-shaped, catalase positive bacteria commonly found in veterinary settings.

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