Lodish Ch 14: Vesicular Traffic and Secretion Mechanisms

The discovery of green fluorescent protein (GFP) has greatly facilitated living cell experiments because:

Endoglycosidase D is a useful reagent because it allows scientists to distinguish glycosylated proteins that:

Yeast sec mutations:

Cell-free transport assays:

The first step in the secretory pathway that should be inhibited by a non-functional mutant of NSF is:

Given the wild type (normal) yeast on the left and the mutant yeast on the right, identify the defective phenotype for the mutant.

Vesicle budding recruits proteins that are needed for subsequent:

The presence of clathrin mediates vesicular transport:

Which of the following small GTPases are NOT involved in vesicle budding or docking?

If Sar1 is inserted into the membrane:

Proteins that function in the ER will encounter which of the following?

What phenotype would be observed in a cell containing a nonhydrolyzable form of ATP with respect to the vesicles of the secretory pathway?

COPI coat proteins mediate ______ transport between the Golgi apparatus and other organelles.

Which of the following is a forward transport sorting signal acting at the ER?

Soluble and membrane proteins advance through the Golgi complex by:

Protein sorting of anterograde cargo to different destinations within the Golgi complex occurs in the:

In hepatocytes, the process by which apically destined proteins travel from the basolateral region across the cell before fusing with the apical membrane is called:

The mannose 6-phosphate residue is important, as it is required to target soluble enzymes to the lysosome. The two enzymes responsible for attaching this residue onto these soluble enzymes reside in the:

In MDCK cells, which of the following is a sorting signal that allows proteins to be targeted to the apical membrane?

The LDL receptor is a receptor for:

Lipoproteins are effective in transporting lipid molecules in an aqueous environment because their surface layer is:

Acidification of endosomes is important in dissociating:

Formation of the late endosome/multivesicular endosome occurs by mechanisms similar to those of:

An important molecule for generating fatty acids in the cell enters via receptor-mediated endocytosis. The complex formed between the receptor on the plasma membrane and the important molecule is stable only at neutral pH. Based on this knowledge, you would predict:

The topogenic sequence of transferrin must include:

Receptor-mediated endocytosis of iron-carrying transferrin results in:

The mannose 6-phosphate residue is important, as it is required to target soluble enzymes to the lysosome. The two enzymes responsible for attaching this residue onto these soluble enzymes reside in the trans-Golgi.

Which of the following is TRUE about lysosomes?