Podcast
Questions and Answers
Which enzymes are involved in the recognition and synapsis of DNA ends during V(D)J recombination?
Which enzymes are involved in the recognition and synapsis of DNA ends during V(D)J recombination?
- TdT
- DNA ligase IV/XRCC4
- Artemis/DNA-PKcs/Ku79/Ku80 (correct)
- DNA-PKcs/Ku79/Ku80
What is the function of TdT in V(D)J recombination?
What is the function of TdT in V(D)J recombination?
- Ligate cut DNA ends
- Add random nucleotides to coding ends (correct)
- Remove unpaired bases from coding ends
- Recognize RSS sequences
What is the role of Artemis in V(D)J recombination?
What is the role of Artemis in V(D)J recombination?
- Joining DNA ends
- Removing unpaired bases from coding ends
- Opening coding end hairpins (correct)
- Adding extra nucleotides at VDJ junctions
Which proteins form a complex that recognizes and closes DNA ends during V(D)J recombination?
Which proteins form a complex that recognizes and closes DNA ends during V(D)J recombination?
What initiates the DNA cleavage at randomly selected RSS during V(D)J recombination?
What initiates the DNA cleavage at randomly selected RSS during V(D)J recombination?
Which process involves the formation of coding joints and signal end joints during V(D)J recombination?
Which process involves the formation of coding joints and signal end joints during V(D)J recombination?
Which enzyme is responsible for joining DNA ends by aligning them and recruiting TdT enzyme?
Which enzyme is responsible for joining DNA ends by aligning them and recruiting TdT enzyme?
'Real' Ig Light chain pairs with the Ig Heavy chain to form what complex?
'Real' Ig Light chain pairs with the Ig Heavy chain to form what complex?
'Surrogate Light Chains' pair with which chain to form the pre-B cell receptor?
'Surrogate Light Chains' pair with which chain to form the pre-B cell receptor?
'Class switch recombination' directs changes in the constant region of antibodies under the influence of which cells?
'Class switch recombination' directs changes in the constant region of antibodies under the influence of which cells?
What is responsible for adding extra (random) nucleotides to broken ends at VDJ junctions mostly in Heavy chains?
What is responsible for adding extra (random) nucleotides to broken ends at VDJ junctions mostly in Heavy chains?
