Urinalysis Basics and Techniques
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Questions and Answers

What is the normal range for urine specific gravity (SG) in most domestic species?

  • 1.015 - 1.045 (correct)
  • 0.990 - 1.020
  • 1.008 - 1.012
  • 1.050 - 1.070
  • Which of the following conditions is NOT associated with increased urine specific gravity (SG)?

  • Increased urine solutes
  • Dehydration
  • Renal failure (correct)
  • Diabetes mellitus
  • What is the condition called when urine SG is similar to plasma and is not more concentrated or diluted?

  • Hyperstenuria
  • Isosthenuria (correct)
  • Hypostenuria
  • Oliguria
  • Which of the following factors can contribute to an ammonia odor in fresh urine?

    <p>Bacterial cystitis with urea-splitting organisms (C)</p> Signup and view all the answers

    What can cause a sweet and fruity odor in urine?

    <p>Presence of ketones (C)</p> Signup and view all the answers

    Which of the following is NOT a formed element that can cause lack of transparency in urine?

    <p>Fat (B)</p> Signup and view all the answers

    What does the presence of excessive foam that is slow to dissipate in urine suggest?

    <p>Proteinuria (B)</p> Signup and view all the answers

    Which of the following methods for determining urine specific gravity is the most accurate?

    <p>Refractometer (C)</p> Signup and view all the answers

    What is the term for a decrease in urine volume?

    <p>Oliguria (D)</p> Signup and view all the answers

    Which of the following is considered a physiological cause of polyuria?

    <p>Increased fluid consumption (C)</p> Signup and view all the answers

    Which of the following is NOT a factor influencing urine volume?

    <p>Blood pressure (D)</p> Signup and view all the answers

    What is the relationship between urine volume and specific gravity?

    <p>High urine volume correlates with low specific gravity (D)</p> Signup and view all the answers

    What is the difference between polyuria and pollakiuria?

    <p>Pollakiuria is frequent urination, while polyuria is increased urine volume (C)</p> Signup and view all the answers

    Which condition is characterized by the pancreas not producing insulin?

    <p>Diabetes mellitus (C)</p> Signup and view all the answers

    Which of the following is NOT a potential cause of polyuria?

    <p>Hypothyroidism (A)</p> Signup and view all the answers

    Which of these substances is NOT involved in regulating urine volume?

    <p>Thyroid hormone (B)</p> Signup and view all the answers

    Which type of crystal is commonly associated with Feline Lower Urinary Tract Disease (FLUTD) in cats?

    <p>Struvite (A), Calcium Oxalate Dihydrate (B)</p> Signup and view all the answers

    Which crystal type is commonly found in herbivores, contributing to the cloudy appearance of their urine?

    <p>Calcium Carbonate (A)</p> Signup and view all the answers

    What crystal type is associated with a potential issue in liver function when found in felines, equines, bovines, and camelids?

    <p>Bilirubin (C)</p> Signup and view all the answers

    Which crystal type is commonly observed in Dalmatians due to their inability to convert uric acid to allantoin?

    <p>Uric Acid (A)</p> Signup and view all the answers

    Which crystal type can be associated with ethylene glycol toxicity (antifreeze) and may exhibit a "picket fence" formation?

    <p>Calcium Oxalate Monohydrate (C)</p> Signup and view all the answers

    Which crystal type is commonly found in acidic urine and can be a sign of disorders of protein metabolism in dogs?

    <p>Cystine (C)</p> Signup and view all the answers

    Which crystal type is commonly associated with liver disease in humans but is less common in dogs and cats?

    <p>Leucine and Tyrosine (C)</p> Signup and view all the answers

    Which of these crystals are typically found in alkaline urine? (Select all that apply)

    <p>Struvite (A), Calcium Carbonate (B)</p> Signup and view all the answers

    What conditions can cause a decreased pH (acidic) in urine?

    <p>High-protein diet (A), Certain medications (C)</p> Signup and view all the answers

    What is the normal urine pH range for a horse?

    <p>8 (C)</p> Signup and view all the answers

    Which of the following is NOT a cause of physiological proteinuria?

    <p>Glomerulonephritis (A)</p> Signup and view all the answers

    What is the significance of interpreting proteinuria in conjunction with urine specific gravity (SG)?

    <p>High protein with low SG indicates more severe protein loss. (B)</p> Signup and view all the answers

    What type of proteinuria occurs due to increased permeability of the glomerulus or impaired reabsorption of protein by the renal tubules?

    <p>Renal (B)</p> Signup and view all the answers

    What is the main reason for a false-positive glucose test on a reagent strip?

    <p>Presence of certain medications (C)</p> Signup and view all the answers

    What is the approximate renal threshold for glucose in cats?

    <p>250 mg/dL (B)</p> Signup and view all the answers

    Which of the following conditions can cause pathologic hyperglycemic glucosuria?

    <p>Diabetes mellitus (B)</p> Signup and view all the answers

    Which type of proteinuria is most likely to be associated with an inflamed bladder?

    <p>Post-renal (B)</p> Signup and view all the answers

    What is the significance of a positive nitrite test in fresh urine?

    <p>Presence of bacteria (B)</p> Signup and view all the answers

    What is the most likely cause of proteinuria in a dog with a history of strenuous exercise?

    <p>Physiological proteinuria (C)</p> Signup and view all the answers

    Which of the following is NOT a factor that influences the presence of glucose in urine?

    <p>Urine specific gravity (A)</p> Signup and view all the answers

    What is the name of the condition where protein is found in the urine due to excessive muscle damage?

    <p>Myoglobinuria (D)</p> Signup and view all the answers

    Which of the following medications can cause false-positive results on a reagent strip glucose test?

    <p>All of the above (D)</p> Signup and view all the answers

    What factor may cause a very dilute urine to result in a false-negative reading for protein on a reagent strip?

    <p>Low protein levels (C)</p> Signup and view all the answers

    In which species is mucus in urine considered normal?

    <p>Horses (C)</p> Signup and view all the answers

    What is the significance of finding sperm in a urine sample?

    <p>Can be a contaminant from recent breeding or a neutered male (A)</p> Signup and view all the answers

    What is the most common site of bacterial infection in the urinary system?

    <p>Bladder (B)</p> Signup and view all the answers

    Which of the following urinary system parasites is considered the largest nematode?

    <p>Dioctophyma renale (C)</p> Signup and view all the answers

    Which of the following is NOT a possible source of fecal or genital contamination in urine samples?

    <p>Dioctophyma renale (B)</p> Signup and view all the answers

    Which of the following is a characteristic of yeast in urine samples?

    <p>Round to oval shape with budding (A)</p> Signup and view all the answers

    Which of the following statements is TRUE about fungi in urine samples?

    <p>They are usually harmless contaminants (A)</p> Signup and view all the answers

    Which of the following conditions could lead to increased levels of urobilinogen in urine?

    <p>All of the above (D)</p> Signup and view all the answers

    What is the primary function of bilirubin in the body?

    <p>To break down red blood cell waste products (B)</p> Signup and view all the answers

    A urine dipstick test reveals the presence of ketones. What is the most likely reason for this finding?

    <p>Incomplete fatty acid metabolism (A)</p> Signup and view all the answers

    Which of the following is a common cause of hematuria?

    <p>Kidney stones (C)</p> Signup and view all the answers

    Which of the following is NOT a potential cause of hemoglobinuria?

    <p>Excessive exercise (A)</p> Signup and view all the answers

    What is the difference between hematuria and hemoglobinuria?

    <p>Hematuria involves intact red blood cells in the urine, while hemoglobinuria involves free hemoglobin. (D)</p> Signup and view all the answers

    Why is it important to collect a fresh urine sample for urobilinogen testing?

    <p>Urobilinogen is a volatile compound that breaks down quickly in light. (C)</p> Signup and view all the answers

    The presence of ketones in the urine can be indicative of:

    <p>All of the above (D)</p> Signup and view all the answers

    A negative nitrite test does not always rule out the presence of nitrate-reducing bacteria in the urine. Why?

    <p>The urine sample must be collected at least four hours after the last urination. (D)</p> Signup and view all the answers

    Why is it recommended to use a microscopic examination to determine the presence of bacteria in urine?

    <p>Microscopy allows for the identification of specific bacterial species. (C)</p> Signup and view all the answers

    What is the purpose of collecting a urine sample for microscopic analysis?

    <p>To identify the presence of cells, casts, and crystals in the urine (A)</p> Signup and view all the answers

    Which of the following best describes the term "occult blood" in urine analysis?

    <p>Blood that is present in the urine but not visible to the naked eye (B)</p> Signup and view all the answers

    Which of the following conditions can cause myoglobinuria?

    <p>Severe muscle damage (C)</p> Signup and view all the answers

    Which of the following tests can be used to differentiate between hemoglobinuria and myoglobinuria?

    <p>80% ammonium sulfate precipitation test (B)</p> Signup and view all the answers

    What is the significance of finding a high level of bilirubin in the urine?

    <p>It indicates a problem with the liver's ability to process bilirubin (C)</p> Signup and view all the answers

    Why is it important to collect urine samples for analysis within a short timeframe?

    <p>All of the above (D)</p> Signup and view all the answers

    What is the primary function of the kidneys in relation to urine formation?

    <p>To filter waste products from the blood (C)</p> Signup and view all the answers

    Flashcards

    Influences on Urine Volume

    Factors that affect the amount of urine produced, including water intake and activity level.

    Specific Gravity (SG)

    A measure of urine concentration; low SG indicates dilute urine, high SG indicates concentrated urine.

    Polyuria (PU)

    Increased urine volume over a 24-hour period; can be due to physiologic or pathologic causes.

    Physiologic Polyuria

    Normal increase in urine volume due to factors like increased fluid intake or medications.

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    Pathologic Polyuria

    Abnormal increase in urine volume caused by diseases like diabetes or renal failure.

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    Oliguria

    Decreased urine volume; can be either a normal response or indicate a health problem.

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    Diabetes Mellitus

    A condition that can lead to pathologic polyuria due to excess glucose in urine.

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    Diabetes Insipidus

    A condition leading to excessive urination caused by a lack of antidiuretic hormone.

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    Cloudy Urine

    Normal, clear urine can become cloudy due to bacterial growth, crystals, or contamination.

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    Strong Odor

    Strong urine odor is normal in certain species, like cats, goats, and pigs.

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    Ammonia Odor

    A strong ammonia smell in fresh urine may indicate bacterial cystitis.

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    Specific Gravity

    The weight of urine compared to distilled water, indicating concentration ability of kidneys.

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    Isosthenuria

    Urine specific gravity similar to plasma, indicating kidneys can't concentrate or dilute.

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    Increased Urine SG

    Indicates dehydration or increased solutes, like protein or glucose.

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    Decreased Urine SG

    Lower SG can indicate renal failure, liver disease, or diabetes.

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    Proteinuria

    Excessive foam in urine indicates protein presence.

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    Crystalluria

    Presence of crystals in urine, often indicating urolithiasis risk.

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    Struvite Crystals

    Crystals formed from magnesium ammonium phosphate, usually in slightly alkaline urine.

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    Amorphous Phosphates

    Granular precipitate throughout urine, found in alkaline conditions.

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    Calcium Oxalate Dihydrate

    Crystals found mainly in acidic to slightly alkaline urine; linked to FLUTD.

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    Uric Acid

    Crystals found in acidic urine; common in Dalmatians due to purine metabolism issues.

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    Cystine Crystals

    Found in acidic urine; linked to cystinuria, rare in dogs and cats.

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    Ammonium Urates

    Crystals appearing in acidic to neutral urine, often tied to liver issues.

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    Bilirubin in Urine

    Presence of bilirubin in acidic urine; normal in dogs, abnormal in others.

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    Reagent Strips

    Strips used in urinalysis to test chemical components in urine.

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    pH in Urine

    Measurement of urine's acidity or alkalinity, influenced by diet.

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    Normal Urine pH for Dogs and Cats

    Normal values are between 5.5 and 7.5 for dogs and cats.

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    Physiologic Proteinuria

    Normal, small amounts of protein in urine due to exertion or stress.

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    Pathologic Proteinuria

    Abnormal protein levels due to medical conditions affecting kidneys.

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    Glucosuria

    Presence of glucose in urine, often indicating high blood glucose levels.

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    Renal Threshold for Glucose

    Blood glucose level at which glucose spills into urine (~180mg/dL for dogs).

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    Physiologic Hyperglycemic Glucosuria

    Glucosuria caused by temporary conditions like stress or exercise.

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    Pathologic Hyperglycemic Glucosuria

    Persistent glucosuria due to conditions like diabetes mellitus.

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    Non-hyperglycemic Glucosuria

    Glucosuria caused by impaired renal tubule function.

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    Nitrite Test

    Positive nitrite in urine indicates bacteria presence, often urinary tract infections.

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    False-Positive Glucosuria

    Conditions or substances that can incorrectly indicate glucose in urine.

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    Expiration of Dipsticks

    The need to monitor and not use expired reagent strips for accurate results.

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    Handling Reagent Strips

    Proper care of dipsticks includes keeping them dry and tightly closed.

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    Mucus in Urine

    Presence in horses is normal; in other species, suggests irritation or contamination.

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    Sperm in Urine

    Common in urine of intact males, recent neutered males, and bred females; usually not significant unless cryptorchid.

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    Lipuria (Fat in Urine)

    Normal in cats; often insignificant and can be due to lubricant contamination during catheterization.

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    Bacteriuria

    Only significant in catheter or cysto samples; if infection is present, leucocytes or erythrocytes will also be seen.

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    Yeast in Urine

    Rare in veterinary patients; appears colorless and round to oval with budding.

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    Fungal Infections

    Rare but serious; often contaminants from outside the body, characterized by distinct hyphae and filaments.

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    Dioctophyma renale

    Giant kidney worm, the largest nematode, can lead to kidney hypertrophy if untreated; requires surgical removal.

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    Tritrichomonas foetus

    Parasitic organism; can come from prepuce contamination, affecting urinary health.

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    Nitrate-reducing bacteria

    Bacteria such as E. coli, Proteus spp., and Klebsiella spp. that convert nitrate to nitrite in urine.

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    Ketonuria

    Presence of ketone bodies in urine due to fatty acid metabolism.

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    Causes of Ketonuria

    Decreased carbohydrate intake, defects in metabolism, or liver issues may lead to ketonuria.

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    Ketoacidosis

    High levels of ketones contributing to acidity in the blood.

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    Bilirubinuria

    Presence of bilirubin in urine, a by-product of red blood cells breakdown.

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    Prehepatic causes for Bilirubinuria

    Conditions leading to increased breakdown of RBCs, like hemolytic anemia.

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    Hepatic causes for Bilirubinuria

    Conditions that affect liver function, leading to bilirubin buildup.

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    Posthepatic causes for Bilirubinuria

    Bile duct obstruction leading to bilirubin accumulation in urine.

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    Urobilinogen

    A by-product of bilirubin conversion in intestines; small amounts in urine is normal.

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    Fresh urine sample for Urobilinogen

    For accurate detection, urine must be fresh and protected from light.

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    Hematuria

    Presence of blood in urine, which can be gross or microscopic.

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    Causes of Hematuria

    Can be due to cystitis, trauma, urinary calculi, or cancer.

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    Hemoglobinuria

    Presence of hemoglobin in urine, indicating RBC destruction.

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    Myoglobinuria

    Presence of myoglobin in urine due to muscle damage.

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    Differentiating Myoglobinuria from Hemoglobinuria

    Use 80% ammonium sulfate precipitation test to distinguish the two.

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    Study Notes

    General Urinalysis Information

    • Urinalysis aids in diagnosing conditions like diabetes, UTIs, and chronic renal failure.
    • It can be used to screen for diseases and monitor disease progression.
    • Urinalysis helps evaluate therapy, such as insulin treatment for diabetes.

    Components of a Urinalysis

    • Gross Exam: Includes color, clarity, volume, and odor of the urine sample.
    • Chemical Analysis: Evaluates dissolved substances in the urine sample.
    • Microscopic Exam: Assesses formed elements within the urine sample.

    Taking a History

    • Obtaining a client history is crucial for accurate interpretation of the urinalysis.
    • Questions should be non-leading and specific, focusing on changes in water consumption, urine output, bladder habits, and gross appearance of the urine.

    Urine Collection Methods

    • Refer to Week 1 lab notes for details on urine collection methods.

    Urinalysis – The Gross Exam

    • Volume: Normal urine production for a normotensive, normovolemic patient with intact renal function is typically 1-2 ml/kg/hour.
    • An example is a 62-pound dog producing 675-1350 ml in 24 hours.
    • Closed systems can monitor volume via urinary catheters, but metabolic cages are often easier to use and are readily available at most clinics.
    • Factors influencing urine volume include water intake, environmental temperature, food intake, level of physical activity, and species of the animal.

    Urine Volume & Specific Gravity (SG)

    • High volume urine usually has a low specific gravity (dilute).
    • Low volume urine typically has a high specific gravity (concentrated).
    • Polyuria is an increase in urine volume measured over a 24-hour period. Normal and appropriate polyuria is considered a physiologic response with low specific gravity and pale urine. Conversely, abnormal polyuria is pathological and indicative of disease with frequent urination.
    • Polyuria is not the same as pollakiuria, which is frequent urination, but total 24-hour volume is considered normal.

    Oliguria

    • Oliguria is a decrease in urine volume that can be either physiologic or pathologic. Physiologic oliguria is due to factors like decreased fluid intake or mild dehydration. Pathologic oliguria may result from dehydration, cardiac disease, renal failure, or shock.

    Anuria

    • Anuria is the complete absence of urine production; this is always considered pathological. Causes include blockage of the urethra or bladder, and end-stage renal failure.

    Urine Color

    • Normal urine color ranges from pale yellow to amber, a by-product of hemoglobin breakdown.
    • Yellowish-brown or greenish-brown urine suggests bile pigments.
    • Reddish-brown urine indicates the presence of red blood cells (hematuria).
    • Brownish-black urine may indicate myoglobin.

    Urine Transparency

    • Transparent urine contains less particulate matter, while slightly cloudy urine indicates slightly more particulate matter.
    • Turbid or cloudy urine suggests formed elements (e.g., crystals, bacteria, cells, or mucus).

    Urine Odor

    • Normal urine can vary in strength, but some species and odors are notable.
    • Strong odor is common in normal males.
    • Ammonia odor suggests bacterial cystitis.
    • Sweet or fruity odor suggests ketones.

    Urine Foam

    • A small amount of white foam is considered normal.
    • Excessive foam suggests proteinuria, and/or bilirubinuria.

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    Explore the fundamental aspects of urinalysis, including its role in diagnosing medical conditions like diabetes and UTIs. Understand the components of urinalysis, including gross, chemical, and microscopic examinations, along with proper urine collection methods. This quiz is essential for anyone looking to deepen their knowledge in medical diagnostics.

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