20 Questions
Which process produces siRNAs that silence complementary sequences?
RNA Interference
What is the main function of synthetic siRNAs?
To produce a transient outcome by targeting mRNA
Which method of vaccine production involves making recombinant gene to produce recombinant protein as vaccine?
Traditional method
What is the role of microRNA (miRNA) in gene expression?
To bind to mRNA and prevent the protein from being translated
Which scientists discovered that base-modified mRNA can be used to block activation of inflammatory reactions?
Kariko & Weissman
What is the precursor of siRNA that forms a hairpin loop?
shRNA
Which of the following is an advantage of using lipid nanoparticles for delivering mRNA vaccines?
Both A and B
What is the main function of PIWI-interacting RNA (piRNA)?
To protect genome integrity from transposable elements
Which companies used lipid nanoparticles with PEG in their mRNA vaccines?
Moderna & Pfizer
What enzyme complex binds to siRNA and attaches to the guide strand?
RISC
What is a limitation of mRNA vaccines?
Need for low temperature
What enzyme is part of the RISC and cleaves and degrades mRNA?
Argonaute
Which organisms are commonly used as eukaryotic model organisms for genome editing?
Mus musculus
What is the function of Dicer in RNA interference?
To produce siRNAs from dsRNA
What is the classic way of genome editing that involves changing DNA at a desired position using a PCR-based technique?
Site-directed mutagenesis
Which type of RNA contains the genetic information for protein translation?
mRNA
What is the purpose of cassette mutagenesis in genome editing?
Introduce variety of mutations to a gene of interest
What is the limitation of synthetic siRNAs as a treatment?
They only produce a transient outcome
What is the difference between random and targeted insertion in genome editing using viral vectors?
Targeted insertion can occur anywhere in the genome
What is the purpose of knockout mice in genome editing?
Study genetic diseases and efficacy of treatments
Test your knowledge on the revolutionary mRNA vaccine technology used by Moderna and Pfizer. Learn about the traditional method of vaccine production and the groundbreaking discovery of base-modified mRNA by Kariko & Weissman. Understand the role of lipid nanoparticles in delivering mRNA vaccines and protecting them from degradation.
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