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Cell Biology and Mycology Basics

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60 Questions

What is the primary function of the Golgi apparatus in a cell?

Sorting, tagging, packaging, and distribution of lipids and proteins

What is the name of the scientific discipline that deals with the study of fungi?

Mycology

What is the term for diseases caused by fungi?

Mycoses

What is the term for the web-like structure of hyphae?

Mycelium

What is the function of substrate mycelia?

Nutrition

What is the term for the study of the structure of fungi?

Morphology

What is the term for the basic element of filamentous fungi?

Hypha

How do fungi obtain their nutrition in the saprophytic mode?

By feeding on dead organic substances

Which classification system of fungi is based on geographic distribution?

Geographic grouping

What is the mode of nutrition of fungi that obtains their nutrition by living on other living organisms?

Parasitic

Which group of fungi is classified based on the mode of transmission of fungal diseases?

Epidemiologic grouping

What is the process by which fungi produce spores?

Reproduction

Which of the following is an example of a fungus that obtains its nutrition by feeding on dead organic substances?

Rhizopus

How many divisions are fungi classified into based on morphologic and cultural characteristics?

Four

What is the primary component that 10-20% KOH dissolves in a skin scraping specimen?

Keratin

Which of the following fungi is responsible for causing Pityriasis versicolor?

Malassezia furfur

What is the primary purpose of incubating fungal cultures for 2-3 weeks?

To allow for fungal growth and maturation

What is the name of the agar medium used to support fungal growth in the laboratory?

Potato dextrose agar (PDA)

Which of the following is NOT a specimen type used in laboratory diagnosis of fungal infections?

Urine

What is the purpose of adding KOH to a skin scraping specimen?

To dissolve keratin and cellular material

Which of the following fungi causes Black piedra?

Piedraia hortae

What type of relationship do symbiotic fungi have with other species?

Mutually beneficial interdependent relationship

What is the role of fungi in mycorrhizal relationships?

To improve nutrient uptake by plants

Which type of fungi has a symbiotic association with algae?

Lichens

What is the characteristic of the hyphae in Zygomycetes?

They are without septa

What is the name of the sexual spores in Ascomycetes?

Ascospores

Which type of fungi is known as sac fungi?

Ascomycetes

What is the mode of asexual reproduction in Basidiomycetes?

All of the above

What is the characteristic of the symbiotic relationship between fungi and algae in lichens?

Fungi provide shelter to algae and algae synthesize carbohydrates for fungi

What is the classification of fungi based on?

Spore formation

What is the purpose of classifying fungi based on epidemiologic grouping?

To understand how fungal diseases are transmitted

What do fungi in the Ascomycota division include?

Yeasts and sac fungi

How do fungi in the saprophytic mode obtain their nutrition?

By feeding on dead organic substances

What is the mode of nutrition of fungi that obtain their nutrition by living on other living organisms?

Parasitic

How many divisions are fungi classified into based on morphologic and cultural characteristics?

Four

What is the term for the mode of nutrition of fungi that obtain their nutrition by feeding on dead organic substances?

Saprophytic

What is the classification of fungi based on geographic distribution?

Geographic grouping

What is the primary function of the endoplasmic reticulum in a cell?

Modifying proteins and synthesizing lipids

What is the characteristic of mycelium?

A network of fungal threads or hyphae

What is the function of peroxisomes?

To break down fatty acids and amino acids

What is the morphology of a hypha?

A branched, tubular structure with a width of 2-10 um

Where do fungi obtain their nutrition?

From the nutrient substrate

What is the function of vesicles and vacuoles?

To store and transport cellular materials

What is the term for scientists who study fungi?

Mycologists

What is the primary function of 10-20% KOH in a skin scraping specimen?

To digest keratin and cellular material

What type of fungi is responsible for causing Pityriasis versicolor?

Malassezia furfur

What is the purpose of incubating fungal cultures for 2-3 weeks?

To allow for fungal growth

What type of relationship do symbiotic fungi have with other species?

Mutually beneficial

What is the characteristic of the hyphae in Zygomycetes?

They are without septa

What type of agar medium is used to support fungal growth in the laboratory?

Potato dextrose agar

What is the primary component of a skin scraping specimen that is digested by 10-20% KOH?

Keratin and cellular material

What is the role of fungi in mycorrhizal relationships?

They improve nutrient uptake by plants

What is the primary function of a KOH mount in laboratory diagnosis?

To digest keratin and cellular material

What is the name of the sexual spores in Ascomycetes?

Ascospores

Which of the following is a specimen type used in laboratory diagnosis of fungal infections?

All of the above

Which type of fungi is known as sac fungi?

Ascomycetes

What is the mode of asexual reproduction in Basidiomycetes?

All of the above

What is the characteristic of the symbiotic relationship between fungi and algae in lichens?

Fungi provide shelter and algae synthesize carbohydrates

What is the classification of fungi based on?

Spore formation

Which type of fungi has a symbiotic association with algae?

Lichens

Study Notes

Cell Biology

  • Endoplasmic reticulum: modifies proteins and synthesizes lipids
  • Golgi apparatus: sorts, tags, packages, and distributes lipids and proteins
  • Peroxisomes: small, round organelles that break down fatty acids and amino acids, and detoxify poisons
  • Vesicles and vacuoles: membrane-bound sacs for storage and transport

Mycology

  • Mycology: scientific discipline dealing with fungi
  • Myco: fungus
  • Mycoses: diseases caused by fungi
  • Medical mycology: study of mycoses and their causes
  • Mycologists: scientists who study fungi

Morphology

  • Hypha: basic element of filamentous fungi, branched, tubular structure, 2-10 um in width
  • Mycelium: web or mat-like structure of hyphae, with crosswalls that form compartments (± cells)

Reproduction

  • Single spore can develop into a mycelium
  • Fruiting bodies of fungi, such as mushrooms, can sprout from a mycelium
  • Spores dispersed widely by wind or water, germinate in a moist place

Classification of Fungi

  • Geographic grouping: classification by geographic distribution
  • Epidemiologic grouping: concerned with how fungal disease is transmitted
  • Taxonomy grouping: scientific grouping according to morphologic and cultural characteristics
  • Divisions: Chytridiomycota, Zygomycota, Ascomycota, and Basidiomycota

Classification of Fungi (continued)

  • Based on mode of nutrition:
    • Saprophytic: obtain nutrition by feeding on dead organic substances
    • Parasitic: obtain nutrition by living on other living organisms
    • Symbiotic: live in a mutually beneficial relationship with other species

Fungal Disease

  • Black piedra: caused by Piedraia hortae
  • White piedra: caused by Trichosporon beigelii
  • Pityriasis versicolor: caused by Malassezia furfur
  • Tinea nigra: caused by Phaeoannellomyces werneckii
  • Candidosis: caused by Candida albicans and Trichophyton
  • Dermatophytosis: caused by Microsporum and Epidermophyton

Laboratory Diagnosis

  • Specimens: skin scrapings, nail clippings, hairs, scrapings from mucous membrane, tissue biopsy, blood, CSF, etc.
  • Microscopy: KOH mount, KOH dissolves keratin and cellular material, leaving fungi intact
  • Fungal growth: cultures incubated for 2-3 weeks, examined macroscopically for colony morphology, and microscopically for fungal morphology

Classification of Fungi (continued)

  • Based on spore formation:
    • Zygomycetes: formed by fusion of two cells, asexual spores are sporangiospores, hyphae are without septa
    • Ascomycetes: also called sac fungi, sexual spores are ascospores, asexual reproduction occurs by conidiospores
    • Basidiomycetes: mushrooms, sexual reproduction occurs by basidiospores, asexual reproduction occurs by conidia, budding, or fragmentation

Cell Biology

  • Endoplasmic reticulum: modifies proteins and synthesizes lipids
  • Golgi apparatus: sorts, tags, packages, and distributes lipids and proteins
  • Peroxisomes: small, round organelles that break down fatty acids and amino acids, and detoxify poisons
  • Vesicles and vacuoles: membrane-bound sacs for storage and transport

Mycology

  • Mycology: scientific discipline dealing with fungi
  • Myco: fungus
  • Mycoses: diseases caused by fungi
  • Medical mycology: study of mycoses and their causes
  • Mycologists: scientists who study fungi

Morphology

  • Hypha: basic element of filamentous fungi, branched, tubular structure, 2-10 um in width
  • Mycelium: web or mat-like structure of hyphae, with crosswalls that form compartments (± cells)

Reproduction

  • Single spore can develop into a mycelium
  • Fruiting bodies of fungi, such as mushrooms, can sprout from a mycelium
  • Spores dispersed widely by wind or water, germinate in a moist place

Classification of Fungi

  • Geographic grouping: classification by geographic distribution
  • Epidemiologic grouping: concerned with how fungal disease is transmitted
  • Taxonomy grouping: scientific grouping according to morphologic and cultural characteristics
  • Divisions: Chytridiomycota, Zygomycota, Ascomycota, and Basidiomycota

Classification of Fungi (continued)

  • Based on mode of nutrition:
    • Saprophytic: obtain nutrition by feeding on dead organic substances
    • Parasitic: obtain nutrition by living on other living organisms
    • Symbiotic: live in a mutually beneficial relationship with other species

Fungal Disease

  • Black piedra: caused by Piedraia hortae
  • White piedra: caused by Trichosporon beigelii
  • Pityriasis versicolor: caused by Malassezia furfur
  • Tinea nigra: caused by Phaeoannellomyces werneckii
  • Candidosis: caused by Candida albicans and Trichophyton
  • Dermatophytosis: caused by Microsporum and Epidermophyton

Laboratory Diagnosis

  • Specimens: skin scrapings, nail clippings, hairs, scrapings from mucous membrane, tissue biopsy, blood, CSF, etc.
  • Microscopy: KOH mount, KOH dissolves keratin and cellular material, leaving fungi intact
  • Fungal growth: cultures incubated for 2-3 weeks, examined macroscopically for colony morphology, and microscopically for fungal morphology

Classification of Fungi (continued)

  • Based on spore formation:
    • Zygomycetes: formed by fusion of two cells, asexual spores are sporangiospores, hyphae are without septa
    • Ascomycetes: also called sac fungi, sexual spores are ascospores, asexual reproduction occurs by conidiospores
    • Basidiomycetes: mushrooms, sexual reproduction occurs by basidiospores, asexual reproduction occurs by conidia, budding, or fragmentation

Learn about the functions of cell organelles such as endoplasmic reticulum, Golgi apparatus, and peroxisomes, as well as the basics of mycology, the study of fungi and fungal diseases.

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