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Questions and Answers
What percentage of the total harvest is processed into chilled, frozen, smoked, dried, fermented, or marinated products?
What percentage of the total harvest is processed into chilled, frozen, smoked, dried, fermented, or marinated products?
What is the approximate percentage of waste generated during shrimp processing?
What is the approximate percentage of waste generated during shrimp processing?
How much shell waste is generated yearly from Argentine red shrimp (ARS) processing?
How much shell waste is generated yearly from Argentine red shrimp (ARS) processing?
What is the approximate percentage of by-products generated from lobster processing?
What is the approximate percentage of by-products generated from lobster processing?
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How much water is required for processing each ton of raw seafood?
How much water is required for processing each ton of raw seafood?
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What is the main nutrient lost during seafood discards?
What is the main nutrient lost during seafood discards?
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What is the approximate percentage of proteins in shrimp head waste?
What is the approximate percentage of proteins in shrimp head waste?
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What is the main component of shell waste from Argentine red shrimp (ARS) processing?
What is the main component of shell waste from Argentine red shrimp (ARS) processing?
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How much wastewater is released daily from one of the largest herring-processing factories in Europe?
How much wastewater is released daily from one of the largest herring-processing factories in Europe?
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What is the water requirement for farmed fish production per metric ton of fish?
What is the water requirement for farmed fish production per metric ton of fish?
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What is the main issue with the seafood industry?
What is the main issue with the seafood industry?
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What are the parts of fish that are commonly discarded?
What are the parts of fish that are commonly discarded?
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What are the environmental hazards associated with seafood waste?
What are the environmental hazards associated with seafood waste?
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What is the main component of the wastewater discharged by the seafood industry?
What is the main component of the wastewater discharged by the seafood industry?
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What are the by-catch of commercial fishing operations?
What are the by-catch of commercial fishing operations?
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What is the significance of the nutrients present in seafood waste?
What is the significance of the nutrients present in seafood waste?
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What is the author's affiliation?
What is the author's affiliation?
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What is the journal where the article was published?
What is the journal where the article was published?
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What is the main advantage of biological treatments for seafood waste?
What is the main advantage of biological treatments for seafood waste?
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What is the primary component of seafood waste?
What is the primary component of seafood waste?
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How much of global seafood production was wasted in 2018?
How much of global seafood production was wasted in 2018?
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What is the projected global seafood production for human consumption by 2030?
What is the projected global seafood production for human consumption by 2030?
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What is the main reason for the generation of seafood waste?
What is the main reason for the generation of seafood waste?
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What percentage of fish stocks were within biologically sustainable levels in 2017?
What percentage of fish stocks were within biologically sustainable levels in 2017?
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What is the environmental impact of landfill compared to other waste disposal options?
What is the environmental impact of landfill compared to other waste disposal options?
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What is the estimated amount of food waste globally?
What is the estimated amount of food waste globally?
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What is the primary purpose of bioconversion processes?
What is the primary purpose of bioconversion processes?
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What is the effect of TSS and FOG of process effluents on the environment?
What is the effect of TSS and FOG of process effluents on the environment?
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What is one of the environmental hazards associated with seafood process effluents?
What is one of the environmental hazards associated with seafood process effluents?
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What is the estimated percentage of food loss and waste for the whole fisheries sector?
What is the estimated percentage of food loss and waste for the whole fisheries sector?
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What is the goal of the United Nations Conference on Sustainable Development regarding food waste?
What is the goal of the United Nations Conference on Sustainable Development regarding food waste?
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What is the main benefit of using microbial fermentations for bioconversion processes?
What is the main benefit of using microbial fermentations for bioconversion processes?
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What is a limitation of conventional processes for valorization of seafood discards and effluents?
What is a limitation of conventional processes for valorization of seafood discards and effluents?
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What is the effect of alkali treatment on chitin?
What is the effect of alkali treatment on chitin?
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What is a drawback of traditional solvent extraction of fish oil?
What is a drawback of traditional solvent extraction of fish oil?
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What is the goal of sustainable seafood processing?
What is the goal of sustainable seafood processing?
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What is a measure for addressing environmental hazards and nutrient losses in the seafood industry?
What is a measure for addressing environmental hazards and nutrient losses in the seafood industry?
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What is the benefit of valorizing seafood processing discards and effluents?
What is the benefit of valorizing seafood processing discards and effluents?
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What is the main advantage of biological processes in waste treatment and resource recovery?
What is the main advantage of biological processes in waste treatment and resource recovery?
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What determines the choice of green extraction technique?
What determines the choice of green extraction technique?
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What is the potential application of enzymes in food quality assessment?
What is the potential application of enzymes in food quality assessment?
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What is the main purpose of bioconversion reactions?
What is the main purpose of bioconversion reactions?
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What is a characteristic of enzymatic techniques in seafood processing?
What is a characteristic of enzymatic techniques in seafood processing?
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What are the two major bioconversion processes?
What are the two major bioconversion processes?
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What is a type of enzyme commonly used in biotechnology?
What is a type of enzyme commonly used in biotechnology?
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What is the role of microorganisms in microbe-mediated bioconversions?
What is the role of microorganisms in microbe-mediated bioconversions?
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What can be recovered from seafood processing discards through bioconversion processes?
What can be recovered from seafood processing discards through bioconversion processes?
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What is the main advantage of fermentation?
What is the main advantage of fermentation?
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What is a green technique for extracting compounds from seafood processing by-products?
What is a green technique for extracting compounds from seafood processing by-products?
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What are some compounds that can be extracted from seafood processing discards?
What are some compounds that can be extracted from seafood processing discards?
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What type of fermentation has been used for the development of fermented fishery products?
What type of fermentation has been used for the development of fermented fishery products?
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What is a potential application of bioactive peptides derived from seafood processing discards?
What is a potential application of bioactive peptides derived from seafood processing discards?
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What is the main factor that affects the efficiency of lactic acid fermentation?
What is the main factor that affects the efficiency of lactic acid fermentation?
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What is the novel approach in bioconversion reactions?
What is the novel approach in bioconversion reactions?
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What is a type of reactor used in downstream processing for the recovery of seafood components?
What is a type of reactor used in downstream processing for the recovery of seafood components?
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What is the result of microbial fermentation of waste?
What is the result of microbial fermentation of waste?
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What is the goal of valorization of seafood processing discards and effluents?
What is the goal of valorization of seafood processing discards and effluents?
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What is a benefit of using enzymes in seafood processing?
What is a benefit of using enzymes in seafood processing?
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What is the primary purpose of bioprocessing of seafood processing discards?
What is the primary purpose of bioprocessing of seafood processing discards?
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What is the main component of shell waste from Argentine red shrimp (ARS) processing?
What is the main component of shell waste from Argentine red shrimp (ARS) processing?
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What is the goal of isoelectric solubilization precipitation (ISP) process?
What is the goal of isoelectric solubilization precipitation (ISP) process?
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What is the benefit of using biological treatments for seafood waste?
What is the benefit of using biological treatments for seafood waste?
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What is the significance of proteins recovered from seafood processing discards?
What is the significance of proteins recovered from seafood processing discards?
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What is the main advantage of using chitosan and its oligosaccharides?
What is the main advantage of using chitosan and its oligosaccharides?
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What is the goal of valorizing seafood processing discards and effluents?
What is the goal of valorizing seafood processing discards and effluents?
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What is the significance of gelatin in food products?
What is the significance of gelatin in food products?
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What is the benefit of bioconversion processes for protein recovery?
What is the benefit of bioconversion processes for protein recovery?
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What is the significance of fish oil in food products?
What is the significance of fish oil in food products?
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What is the primary role of lactic acid formed during sugar breakdown in seafood?
What is the primary role of lactic acid formed during sugar breakdown in seafood?
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What is the benefit of fed-batch operation over conventional batch operation?
What is the benefit of fed-batch operation over conventional batch operation?
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What is the primary function of chitinases in microbial growth?
What is the primary function of chitinases in microbial growth?
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What is the primary product of fermentation by lactic acid bacteria (LAB)?
What is the primary product of fermentation by lactic acid bacteria (LAB)?
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What is the result of microbial fermentation on seafood proteins?
What is the result of microbial fermentation on seafood proteins?
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What is the primary benefit of microbial fermentation in seafood processing?
What is the primary benefit of microbial fermentation in seafood processing?
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What is the primary application of microbial fermentation in seafood processing?
What is the primary application of microbial fermentation in seafood processing?
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What is the primary role of lipases in microbial growth?
What is the primary role of lipases in microbial growth?
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What is the primary advantage of using microbial fermentation for seafood processing?
What is the primary advantage of using microbial fermentation for seafood processing?
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What is the primary result of fermentation of shrimp shell waste by lactic acid bacteria?
What is the primary result of fermentation of shrimp shell waste by lactic acid bacteria?
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What is the primary role of microorganisms in seafood processing?
What is the primary role of microorganisms in seafood processing?
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What is the result of the microbe-assisted aerobic bioprocess of aquaculture solid waste?
What is the result of the microbe-assisted aerobic bioprocess of aquaculture solid waste?
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What is the benefit of fed-batch biodegradation over batch biodegradation?
What is the benefit of fed-batch biodegradation over batch biodegradation?
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What is the role of aerobic continuous bioreactor in treating high saline fish processing wastewater?
What is the role of aerobic continuous bioreactor in treating high saline fish processing wastewater?
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What is the main component of the anaerobic digestion process?
What is the main component of the anaerobic digestion process?
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What is the function of algae in the bioprocess?
What is the function of algae in the bioprocess?
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What is the result of the integration of anaerobic digestion and activated sludge aerated bioreactor?
What is the result of the integration of anaerobic digestion and activated sludge aerated bioreactor?
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What is the function of microorganisms in the activated sludge system?
What is the function of microorganisms in the activated sludge system?
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What is the role of dissolved air flotation system in anaerobic digestion?
What is the role of dissolved air flotation system in anaerobic digestion?
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What is the benefit of fermentation over chemical treatment for the extraction of collagen?
What is the benefit of fermentation over chemical treatment for the extraction of collagen?
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What is the major advantage of open ponds for cultivating phototrophic algae?
What is the major advantage of open ponds for cultivating phototrophic algae?
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What is the major limitation of heterotrophic cultivation in closed systems?
What is the major limitation of heterotrophic cultivation in closed systems?
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What is the approximate percentage of proteins in algal mass (SCP)?
What is the approximate percentage of proteins in algal mass (SCP)?
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What is the key advantage of microalgae for producing third-generation biofuel?
What is the key advantage of microalgae for producing third-generation biofuel?
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What is the benefit of using microalgae for bioconversions of food, fishery, and agricultural waste?
What is the benefit of using microalgae for bioconversions of food, fishery, and agricultural waste?
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What is the approximate carbon fixation rate of microalgae cultivation in wastewater?
What is the approximate carbon fixation rate of microalgae cultivation in wastewater?
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What is the advantage of using consortium of hydrolytic enzymes for degrading crab shells, prawn shells, and fish scales?
What is the advantage of using consortium of hydrolytic enzymes for degrading crab shells, prawn shells, and fish scales?
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What is the benefit of using microalgae as a source of SCP for replacing expensive soy meal and fishmeal in animal and aquaculture feeds?
What is the benefit of using microalgae as a source of SCP for replacing expensive soy meal and fishmeal in animal and aquaculture feeds?
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What is the purpose of adding a weak acid to the washed meat mince?
What is the purpose of adding a weak acid to the washed meat mince?
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What is the estimated water requirement for producing microalgae as SCP?
What is the estimated water requirement for producing microalgae as SCP?
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What is the typical temperature range used for incubation during enzymatic hydrolysis of seafood processing discards?
What is the typical temperature range used for incubation during enzymatic hydrolysis of seafood processing discards?
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What is the advantage of using anaerobic digestion of seafood processing wastewater by Chlorella sp. for biogas production?
What is the advantage of using anaerobic digestion of seafood processing wastewater by Chlorella sp. for biogas production?
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What is the purpose of using food-grade polysaccharides in flocculation and precipitation of proteins?
What is the purpose of using food-grade polysaccharides in flocculation and precipitation of proteins?
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What is the result of mild heating to 50°C during the preparation of thermo-stable protein dispersions?
What is the result of mild heating to 50°C during the preparation of thermo-stable protein dispersions?
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What is a potential application of thermo-stable protein dispersions prepared from fishery products?
What is a potential application of thermo-stable protein dispersions prepared from fishery products?
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What is the primary component of the concentrate obtained from cooking wastewaters of snow crab?
What is the primary component of the concentrate obtained from cooking wastewaters of snow crab?
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What is the benefit of enzymatic hydrolysis of seafood processing discards?
What is the benefit of enzymatic hydrolysis of seafood processing discards?
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What is a characteristic of fish frames treated with commercial proteases?
What is a characteristic of fish frames treated with commercial proteases?
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What is the purpose of ultrafiltration and electroflocculation in protein recovery?
What is the purpose of ultrafiltration and electroflocculation in protein recovery?
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What is a benefit of using enzymatically hydrolyzed proteins as functional additives?
What is a benefit of using enzymatically hydrolyzed proteins as functional additives?
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What is the minimum protein content of Fish Protein Isolates (FPIs)?
What is the minimum protein content of Fish Protein Isolates (FPIs)?
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What is the primary difference between Fish Protein Isolates (FPIs) and surimi?
What is the primary difference between Fish Protein Isolates (FPIs) and surimi?
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What is the potential application of collagen-based novel functional food ingredients?
What is the potential application of collagen-based novel functional food ingredients?
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What is the effect of adding collagen hydrolysate (CH) to silver carp protein isolate prior to gelation?
What is the effect of adding collagen hydrolysate (CH) to silver carp protein isolate prior to gelation?
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What is the type of collagen isolated from silver carp?
What is the type of collagen isolated from silver carp?
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What is the application of tilapia type I collagen?
What is the application of tilapia type I collagen?
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What is the pre-treatment step for extracting gelatin from collagen?
What is the pre-treatment step for extracting gelatin from collagen?
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What is the characteristic of gelatin extracted from big eye tuna skin?
What is the characteristic of gelatin extracted from big eye tuna skin?
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What is the process that makes use of the ability of muscle structural proteins to undergo gelation under mild acidic conditions?
What is the process that makes use of the ability of muscle structural proteins to undergo gelation under mild acidic conditions?
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What is the purpose of the mechanical deboning step in the mild acid induced gelation process?
What is the purpose of the mechanical deboning step in the mild acid induced gelation process?
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What is the percentage of crude protein found in the raw heads, shells, and tails of Northern pink shrimp and spotted shrimp?
What is the percentage of crude protein found in the raw heads, shells, and tails of Northern pink shrimp and spotted shrimp?
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What is the major components of seafood processing discards?
What is the major components of seafood processing discards?
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What is the average percentage of calcium in seafood processing discards?
What is the average percentage of calcium in seafood processing discards?
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What is the primary environmental concern with seafood processing effluents?
What is the primary environmental concern with seafood processing effluents?
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What is the estimated annual loss of proteins in the US due to seafood discards?
What is the estimated annual loss of proteins in the US due to seafood discards?
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What is the primary method of disposing solid waste from seafood processing?
What is the primary method of disposing solid waste from seafood processing?
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What is the environmental impact of ocean dumping of by-catch?
What is the environmental impact of ocean dumping of by-catch?
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What is the estimated annual cost of disposing lobster processing waste?
What is the estimated annual cost of disposing lobster processing waste?
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What is the limitation of composting and ensilage of seafood waste?
What is the limitation of composting and ensilage of seafood waste?
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What is the result of anaerobic decomposition of seafood waste in landfills?
What is the result of anaerobic decomposition of seafood waste in landfills?
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What percentage of the raw material is discarded as by-products during finfish processing?
What percentage of the raw material is discarded as by-products during finfish processing?
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What is the estimated amount of shell waste generated yearly from Argentine red shrimp (ARS) processing?
What is the estimated amount of shell waste generated yearly from Argentine red shrimp (ARS) processing?
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What is the approximate percentage of the edible seafood supply that is not available for human consumption?
What is the approximate percentage of the edible seafood supply that is not available for human consumption?
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What is the main component of the wastewater discharged by the seafood industry?
What is the main component of the wastewater discharged by the seafood industry?
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What is the estimated amount of wastewater released daily from one of the largest herring-processing factories in Europe?
What is the estimated amount of wastewater released daily from one of the largest herring-processing factories in Europe?
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What is the approximate percentage of chitin in shrimp head waste?
What is the approximate percentage of chitin in shrimp head waste?
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What is the estimated amount of solid waste generated from freshwater fish processing?
What is the estimated amount of solid waste generated from freshwater fish processing?
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What is the estimated amount of water required for processing each ton of raw seafood?
What is the estimated amount of water required for processing each ton of raw seafood?
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What is the approximate percentage of proteins in seafood processing discards?
What is the approximate percentage of proteins in seafood processing discards?
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What is the estimated amount of shell waste generated yearly from shrimp and crab processing in the EU?
What is the estimated amount of shell waste generated yearly from shrimp and crab processing in the EU?
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What is the primary source of nutrients in seafood waste?
What is the primary source of nutrients in seafood waste?
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What is the environmental impact of seafood waste?
What is the environmental impact of seafood waste?
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What is the purpose of valorizing seafood processing discards and effluents?
What is the purpose of valorizing seafood processing discards and effluents?
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What is the role of bioconversion processes in seafood waste management?
What is the role of bioconversion processes in seafood waste management?
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What is the main advantage of biological treatments for seafood waste?
What is the main advantage of biological treatments for seafood waste?
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What is the significance of the nutrients present in seafood waste?
What is the significance of the nutrients present in seafood waste?
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What is the main goal of sustainable seafood processing?
What is the main goal of sustainable seafood processing?
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What is the benefit of using microbial fermentations for bioconversion processes?
What is the benefit of using microbial fermentations for bioconversion processes?
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What is the environmental impact of landfill compared to other waste disposal options?
What is the environmental impact of landfill compared to other waste disposal options?
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What is the effect of TSS and FOG of process effluents on the environment?
What is the effect of TSS and FOG of process effluents on the environment?
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What is the primary reason for the generation of seafood waste?
What is the primary reason for the generation of seafood waste?
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What is the projected global seafood production for human consumption by 2030?
What is the projected global seafood production for human consumption by 2030?
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What is the goal of sustainable seafood processing?
What is the goal of sustainable seafood processing?
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What is a limitation of conventional processes for valorization of seafood discards and effluents?
What is a limitation of conventional processes for valorization of seafood discards and effluents?
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What is the main advantage of biological treatments for seafood waste?
What is the main advantage of biological treatments for seafood waste?
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What is the effect of alkali treatment on chitin?
What is the effect of alkali treatment on chitin?
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What is the estimated percentage of fish stocks within biologically sustainable levels in 2017?
What is the estimated percentage of fish stocks within biologically sustainable levels in 2017?
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What is a drawback of traditional solvent extraction of fish oil?
What is a drawback of traditional solvent extraction of fish oil?
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What is the primary purpose of bioconversion processes?
What is the primary purpose of bioconversion processes?
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What is the significance of the nutrients present in seafood waste?
What is the significance of the nutrients present in seafood waste?
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What is the main benefit of valorizing seafood processing discards and effluents?
What is the main benefit of valorizing seafood processing discards and effluents?
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What is the goal of the United Nations Conference on Sustainable Development regarding food waste?
What is the goal of the United Nations Conference on Sustainable Development regarding food waste?
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What is the main benefit of valorizing seafood processing discards and effluents?
What is the main benefit of valorizing seafood processing discards and effluents?
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What is the estimated amount of food waste globally?
What is the estimated amount of food waste globally?
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What is a measure for addressing environmental hazards and nutrient losses in the seafood industry?
What is a measure for addressing environmental hazards and nutrient losses in the seafood industry?
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What is the main advantage of using microbial fermentations for bioconversion processes?
What is the main advantage of using microbial fermentations for bioconversion processes?
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What is the main advantage of biological processes in waste treatment and resource recovery?
What is the main advantage of biological processes in waste treatment and resource recovery?
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What is the primary goal of sustainable seafood processing?
What is the primary goal of sustainable seafood processing?
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What is the approximate percentage of lipid content in the raw heads, shells, and tails of Northern pink shrimp and spotted shrimp?
What is the approximate percentage of lipid content in the raw heads, shells, and tails of Northern pink shrimp and spotted shrimp?
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What is the major component of total suspended solids (TSS) in seafood processing effluents?
What is the major component of total suspended solids (TSS) in seafood processing effluents?
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What is the environmental impact of ocean dumping of seafood processing waste?
What is the environmental impact of ocean dumping of seafood processing waste?
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What is the estimated annual loss of proteins in the US seafood industry due to discards?
What is the estimated annual loss of proteins in the US seafood industry due to discards?
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What is the approximate percentage of proteins in lobster liver?
What is the approximate percentage of proteins in lobster liver?
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What is the major environmental hazard associated with anaerobic decomposition of seafood waste in landfills?
What is the major environmental hazard associated with anaerobic decomposition of seafood waste in landfills?
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What is the benefit of valorizing seafood processing discards and effluents?
What is the benefit of valorizing seafood processing discards and effluents?
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What is the approximate percentage of crude protein in the solid discards of seafood processing?
What is the approximate percentage of crude protein in the solid discards of seafood processing?
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What is the major component of the characteristic features of seafood processing effluents?
What is the major component of the characteristic features of seafood processing effluents?
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What is the estimated annual loss of n-3-PUFAs in the US seafood industry due to discards?
What is the estimated annual loss of n-3-PUFAs in the US seafood industry due to discards?
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What is the main advantage associated with cultivating phototrophic algae in open ponds?
What is the main advantage associated with cultivating phototrophic algae in open ponds?
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What is the primary application of single-cell proteins (SCP) produced from microalgae?
What is the primary application of single-cell proteins (SCP) produced from microalgae?
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What is the estimated water requirement for cultivating microalgae?
What is the estimated water requirement for cultivating microalgae?
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What is the benefit of cultivating microalgae in wastewater?
What is the benefit of cultivating microalgae in wastewater?
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What is the significance of microalgae in biofuel production?
What is the significance of microalgae in biofuel production?
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What is the benefit of using microorganisms for bioconversions of food, fishery, and agricultural waste?
What is the benefit of using microorganisms for bioconversions of food, fishery, and agricultural waste?
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What is the role of microorganisms in microbe-mediated bioconversions?
What is the role of microorganisms in microbe-mediated bioconversions?
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What is a significant advantage of using enzymes in seafood processing?
What is a significant advantage of using enzymes in seafood processing?
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What is the advantage of direct transformation of lipidic biomass into biodiesel?
What is the advantage of direct transformation of lipidic biomass into biodiesel?
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What is the benefit of anaerobic digestion of seafood processing wastewater by Chlorella sp.?
What is the benefit of anaerobic digestion of seafood processing wastewater by Chlorella sp.?
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What is a common application of enzymatic hydrolysis of protein from aquatic by-products?
What is a common application of enzymatic hydrolysis of protein from aquatic by-products?
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What is a major class of compounds that can be extracted from seafood processing discards?
What is a major class of compounds that can be extracted from seafood processing discards?
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What is the significance of microalgae cultivation in closed systems?
What is the significance of microalgae cultivation in closed systems?
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What is a green technique for extracting compounds from seafood processing by-products?
What is a green technique for extracting compounds from seafood processing by-products?
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What is a benefit of valorizing seafood processing discards and effluents?
What is a benefit of valorizing seafood processing discards and effluents?
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What is a characteristic of enzymatic techniques in seafood processing?
What is a characteristic of enzymatic techniques in seafood processing?
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What is a potential application of enzymes in food quality assessment?
What is a potential application of enzymes in food quality assessment?
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What can be recovered from seafood processing discards through bioconversion processes?
What can be recovered from seafood processing discards through bioconversion processes?
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What is the main advantage of biological processes in waste treatment and resource recovery?
What is the main advantage of biological processes in waste treatment and resource recovery?
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What is a goal of sustainable seafood processing?
What is a goal of sustainable seafood processing?
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What is the primary purpose of bioconversion reactions in seafood processing?
What is the primary purpose of bioconversion reactions in seafood processing?
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What is a characteristic of microbial fermentation in seafood processing?
What is a characteristic of microbial fermentation in seafood processing?
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What determines the choice of green extraction technique for seafood processing by-products?
What determines the choice of green extraction technique for seafood processing by-products?
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What is a limitation of conventional processes for valorization of seafood discards and effluents?
What is a limitation of conventional processes for valorization of seafood discards and effluents?
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What is the main benefit of using microbial fermentations for bioconversion processes?
What is the main benefit of using microbial fermentations for bioconversion processes?
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What is a type of biophysical process that can favor the release of components from food matrices?
What is a type of biophysical process that can favor the release of components from food matrices?
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What is the main advantage of biological processes in waste treatment and resource recovery?
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What is a potential application of bioconversion processes in seafood processing?
What is a potential application of bioconversion processes in seafood processing?
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What is the role of microorganisms in microbe-mediated bioconversions?
What is the role of microorganisms in microbe-mediated bioconversions?
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What is a characteristic of enzymatic techniques in seafood processing?
What is a characteristic of enzymatic techniques in seafood processing?
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What is the primary effect of lactic acid formed during sugar breakdown in seafood processing?
What is the primary effect of lactic acid formed during sugar breakdown in seafood processing?
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What is the main advantage of fed-batch fermentation over conventional batch operation?
What is the main advantage of fed-batch fermentation over conventional batch operation?
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What is the primary function of chitinases in microbial fermentation?
What is the primary function of chitinases in microbial fermentation?
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What is the primary application of microbial fermentation in seafood processing?
What is the primary application of microbial fermentation in seafood processing?
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What is the primary role of lactic acid bacteria in fish fermentation?
What is the primary role of lactic acid bacteria in fish fermentation?
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What is the primary benefit of using microbial fermentation for seafood processing?
What is the primary benefit of using microbial fermentation for seafood processing?
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What is the primary outcome of fermenting shrimp shell waste by symbiotic lactic acid bacteria?
What is the primary outcome of fermenting shrimp shell waste by symbiotic lactic acid bacteria?
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What is the primary application of microbial growth in seafood processing?
What is the primary application of microbial growth in seafood processing?
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What is the primary outcome of applying microbe-assisted bioconversions to seafood processing waste?
What is the primary outcome of applying microbe-assisted bioconversions to seafood processing waste?
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What is the primary advantage of using microorganisms for bioconversion processes?
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What is the minimum protein content of fish protein isolates (FPIs)?
What is the minimum protein content of fish protein isolates (FPIs)?
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What is the main difference between fish protein isolates (FPIs) and surimi?
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What is the application of marine collagen in biomedical and food industries?
What is the application of marine collagen in biomedical and food industries?
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What is the result of adding collagen hydrolysate (CH) to silver carp protein isolate?
What is the result of adding collagen hydrolysate (CH) to silver carp protein isolate?
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What is the biocompatibility of tilapia type I collagen?
What is the biocompatibility of tilapia type I collagen?
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What is the characteristic of gelatin extracted from big eye tuna skin?
What is the characteristic of gelatin extracted from big eye tuna skin?
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What is the principle behind the mild acid induced gelation process?
What is the principle behind the mild acid induced gelation process?
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What is the characteristic of the gelatin extracted from collagen of skins and bones of various marine and freshwater fishery sources?
What is the characteristic of the gelatin extracted from collagen of skins and bones of various marine and freshwater fishery sources?
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What is the significance of collagen and collagen hydrolysate (CH) in food and biomedical applications?
What is the significance of collagen and collagen hydrolysate (CH) in food and biomedical applications?
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What is the advantage of using fish protein isolates (FPIs) as raw materials?
What is the advantage of using fish protein isolates (FPIs) as raw materials?
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What is the primary function of microorganisms in the treatment of seafood industry process effluents?
What is the primary function of microorganisms in the treatment of seafood industry process effluents?
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What is the result of anaerobic digestion of tuna processing effluents?
What is the result of anaerobic digestion of tuna processing effluents?
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What is the purpose of algae-based bioconversions in seafood processing?
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What is the result of fermentation of dried skipjack tuna waste with L. plantarum and B. licheniformis?
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What is the significance of fed-batch biodegradation in the production of target compounds?
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What is the role of microorganisms in microbe-assisted aerobic bioprocess of aquaculture solid waste?
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What is the advantage of fermentation over chemical treatment for the extraction of collagen?
What is the advantage of fermentation over chemical treatment for the extraction of collagen?
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What is the purpose of an aerobic continuous bioreactor in treating high saline fish processing wastewater?
What is the purpose of an aerobic continuous bioreactor in treating high saline fish processing wastewater?
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What is the result of anaerobic digestion of seafood industry effluents in a dissolved air flotation system?
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What is the significance of microbe-assisted aerobic bioprocess of aquaculture solid waste in nitrogen bioconversion?
What is the significance of microbe-assisted aerobic bioprocess of aquaculture solid waste in nitrogen bioconversion?
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What is the primary component of the nitrogenous fraction in seafood processing discards?
What is the primary component of the nitrogenous fraction in seafood processing discards?
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What is the purpose of isoelectric solubilization precipitation (ISP) in protein recovery from seafood discards?
What is the purpose of isoelectric solubilization precipitation (ISP) in protein recovery from seafood discards?
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What is the benefit of using bioconversion processes for protein recovery from seafood discards?
What is the benefit of using bioconversion processes for protein recovery from seafood discards?
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What is the estimated percentage of proteins in seafood processing discards?
What is the estimated percentage of proteins in seafood processing discards?
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What is the purpose of high intensity sonication, electro-flocculation, and ultrafiltration in protein recovery from seafood discards?
What is the purpose of high intensity sonication, electro-flocculation, and ultrafiltration in protein recovery from seafood discards?
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What is the significance of bioavailability in seafood-derived compounds?
What is the significance of bioavailability in seafood-derived compounds?
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What is the main advantage of using chitosan and its oligosaccharides in food products?
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What is the purpose of gelatin in food products?
What is the purpose of gelatin in food products?
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What is the main benefit of using bioconversion processes for valorization of seafood discards and effluents?
What is the main benefit of using bioconversion processes for valorization of seafood discards and effluents?
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What is the main limitation of conventional processes for valorization of seafood discards and effluents?
What is the main limitation of conventional processes for valorization of seafood discards and effluents?
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What are the main constituents of seafood processing discards?
What are the main constituents of seafood processing discards?
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What is the significance of the nutrients present in seafood waste?
What is the significance of the nutrients present in seafood waste?
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What is the main environmental hazard associated with seafood waste?
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What is the main goal of valorizing seafood processing discards and effluents?
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What is the benefit of bioconversion processes for seafood waste?
What is the benefit of bioconversion processes for seafood waste?
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What is the main advantage of biological treatments for seafood waste?
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What is the significance of the by-catch of commercial fishing operations?
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What is the main goal of sustainable seafood processing?
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What is the pH range to which the homogenate is lowered to induce gelation of proteins?
What is the pH range to which the homogenate is lowered to induce gelation of proteins?
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What is the temperature range for mild heating to enhance the viscosity fall during the gelation process?
What is the temperature range for mild heating to enhance the viscosity fall during the gelation process?
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Which of the following fish species is an exception to the thermo-stable protein dispersions prepared from fishery products?
Which of the following fish species is an exception to the thermo-stable protein dispersions prepared from fishery products?
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What is the purpose of using food-grade polysaccharides such as carrageenan, alginate, and carboxy methylcellulose in protein recovery?
What is the purpose of using food-grade polysaccharides such as carrageenan, alginate, and carboxy methylcellulose in protein recovery?
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What is the primary application of thermo-stable protein dispersions prepared from fishery products?
What is the primary application of thermo-stable protein dispersions prepared from fishery products?
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What is the primary method used to recover proteins from herring industry processing effluents?
What is the primary method used to recover proteins from herring industry processing effluents?
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What is the primary component of the concentrate obtained from cooking wastewaters of snow crab?
What is the primary component of the concentrate obtained from cooking wastewaters of snow crab?
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What is the ideal temperature range for incubation during enzymatic hydrolysis of seafood processing discards?
What is the ideal temperature range for incubation during enzymatic hydrolysis of seafood processing discards?
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What is the primary benefit of using enzymatic hydrolysis to extract proteins from seafood processing discards?
What is the primary benefit of using enzymatic hydrolysis to extract proteins from seafood processing discards?
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What is the primary application of fish protein hydrolyzates (FPHs) in the food industry?
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What percentage of the total weight of the Northern pink shrimp and spotted shrimp is accounted for by their crude protein?
What percentage of the total weight of the Northern pink shrimp and spotted shrimp is accounted for by their crude protein?
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What is the range of lipid content in the raw heads, shells, and tails of Northern pink shrimp and spotted shrimp?
What is the range of lipid content in the raw heads, shells, and tails of Northern pink shrimp and spotted shrimp?
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What is the estimated annual loss of n-3 PUFA in the US due to seafood discards?
What is the estimated annual loss of n-3 PUFA in the US due to seafood discards?
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What is the main component of total suspended solids (TSS) in seafood processing effluents?
What is the main component of total suspended solids (TSS) in seafood processing effluents?
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What is the environmental impact of ocean dumping of seafood waste?
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What is the estimated annual cost of disposing of lobster processing waste?
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What is the main drawback of composting and ensilage of seafood waste?
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What is the main environmental impact of anaerobic decomposition of seafood in landfills?
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What is the main component of seafood processing effluents?
What is the main component of seafood processing effluents?
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What is the main reason for the loss of nutrients during seafood processing?
What is the main reason for the loss of nutrients during seafood processing?
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What is the primary reason for the significant amount of solid waste generated in the seafood industry?
What is the primary reason for the significant amount of solid waste generated in the seafood industry?
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What is the estimated percentage of whole shellfish that constitutes waste on a wet weight basis?
What is the estimated percentage of whole shellfish that constitutes waste on a wet weight basis?
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What is the annual amount of shell waste generated from Argentine red shrimp (ARS) processing?
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What is the percentage of edible seafood supply that is not available for human consumption?
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What is the average protein content of seafood processing discards?
What is the average protein content of seafood processing discards?
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What is the amount of water required for processing each ton of raw seafood?
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What is the primary component of shell waste from Argentine red shrimp (ARS) processing?
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What is the main nutrient lost during seafood discards?
What is the main nutrient lost during seafood discards?
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What is the main concern associated with the seafood industry's wastewater discharge?
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What is the estimated amount of shell waste generated from shrimp processing in the EU?
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What is a characteristic of biological processes in waste treatment and resource recovery?
What is a characteristic of biological processes in waste treatment and resource recovery?
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What is the primary purpose of bioconversion reactions?
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What determines the choice of green extraction technique?
What determines the choice of green extraction technique?
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What is a type of bioconversion process that employs externally added enzymes?
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What is a characteristic of microbial fermentation?
What is a characteristic of microbial fermentation?
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What is the role of microorganisms in microbe-mediated bioconversions?
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What is the potential application of bioconversion processes?
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What is a benefit of using biological processes in waste treatment and resource recovery?
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What is a type of green technique used for industrial recovery of biomolecules from seafood by-products?
What is a type of green technique used for industrial recovery of biomolecules from seafood by-products?
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What is the main advantage of bioconversion processes?
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What is the advantage of open ponds for algae cultivation?
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What percentage of proteins can be found in single cell proteins (SCP)?
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What is the main environmental issue associated with landfill disposal of seafood waste?
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What is the benefit of microalgae cultivation in wastewater?
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What is the main goal of sustainable seafood processing?
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What is the application of Bacillus sp., Brevibacterium sp., and Vibrio sp. in seafood processing?
What is the application of Bacillus sp., Brevibacterium sp., and Vibrio sp. in seafood processing?
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What is the limitation of conventional chemical and physical methods for valorization of seafood discards and effluents?
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What is the benefit of using microalgae for biofuel production?
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What is the primary concern of the United Nations Conference on Sustainable Development regarding food waste?
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What is the effect of TSS and FOG of process effluents on the environment?
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What is the effect of biodiesel compared to petroleum diesel?
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What is the application of SCP in animal and aquaculture feeds?
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What is the main benefit of valorizing seafood processing discards and effluents?
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What is the estimated water requirement for microalgae cultivation?
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What is the main advantage of biological treatments for seafood waste?
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What is the benefit of anaerobic digestion of seafood processing wastewater by Chlorella sp.?
What is the benefit of anaerobic digestion of seafood processing wastewater by Chlorella sp.?
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What is the main challenge facing the seafood industry in terms of environmental pollution?
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What is the limitation of alkali treatment on chitin extraction from crustacean shells?
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What is the application of Fadhil et al.'s method for producing liquid biofuels?
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What is the main drawback of traditional solvent extraction of fish oil?
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What was the nitrogen content of the fertilizer produced through solid state fermentation of skipjack tuna waste?
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Which of the following is a potential application of enzymes in seafood processing?
Which of the following is a potential application of enzymes in seafood processing?
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Which microorganisms were used to ferment the dried skipjack tuna waste?
Which microorganisms were used to ferment the dried skipjack tuna waste?
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What is the main advantage of enzymatic techniques in seafood processing?
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What is the primary reason for the generation of seafood waste?
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What was the result of converting fish waste to liquid fertilizer using mixed microorganisms?
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What is the estimated percentage of fish stocks within biologically sustainable levels in 2017?
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What is the main advantage of fermentation over chemical treatment for the extraction of collagen?
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Which of the following compounds can be recovered from seafood processing discards through bioconversion processes?
Which of the following compounds can be recovered from seafood processing discards through bioconversion processes?
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What is the primary purpose of bioconversion processes in seafood waste treatment?
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What is the role of marine biotechnology-based downstream processes in seafood processing?
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What was the result of fed-batch biodegradation of shrimp-shell powder with B.cereus?
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What is the projected global seafood production for human consumption by 2030?
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What is the main purpose of bioconversion reactions in seafood processing?
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What is the role of microorganisms in the activated sludge system for treating seafood industry process effluents?
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What is the main advantage of biological treatments for seafood waste?
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What is the result of anaerobic digestion of tuna processing effluents?
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What is a characteristic of hydrolases, a popular enzyme in biotechnology?
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What is the estimated amount of food waste globally?
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What is the role of microalgae in bioprocessing of seafood industry discards?
What is the role of microalgae in bioprocessing of seafood industry discards?
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What is the main advantage of using microwave-assisted extraction for recovering compounds from seafood processing by-products?
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What can be produced from seafood discards using enzymatic hydrolysis?
What can be produced from seafood discards using enzymatic hydrolysis?
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What is the main benefit of using microbial fermentations for bioconversion processes?
What is the main benefit of using microbial fermentations for bioconversion processes?
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What is the significance of the bioconversion process in seafood processing?
What is the significance of the bioconversion process in seafood processing?
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What is the primary component of seafood waste?
What is the primary component of seafood waste?
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What is the benefit of using algae-based bioconversions for seafood industry effluents?
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What is the potential application of bioactive compounds extracted from seafood processing discards?
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What is the role of microorganisms in microbe-mediated bioconversions?
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What is the main purpose of downstream processing in seafood processing?
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What is the benefit of valorizing seafood processing discards and effluents?
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What is the minimum protein content of fish protein isolates (FPIs)?
What is the minimum protein content of fish protein isolates (FPIs)?
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What type of collagen is isolated from silver carp?
What type of collagen is isolated from silver carp?
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What is the primary function of collagen-based novel functional food ingredients?
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What is the purpose of adding collagen hydrolysate (CH) to silver carp protein isolate?
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What is the application of tilapia type I collagen?
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What is the primary component of gelatin extracted from collagen?
What is the primary component of gelatin extracted from collagen?
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What is the process of extracting gelatin from collagen?
What is the process of extracting gelatin from collagen?
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What is the purpose of mild acid induced gelation?
What is the purpose of mild acid induced gelation?
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What is the difference between fish protein isolates (FPIs) and surimi?
What is the difference between fish protein isolates (FPIs) and surimi?
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What is the benefit of using collagen and collagen hydrolysate (CH) in food applications?
What is the benefit of using collagen and collagen hydrolysate (CH) in food applications?
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What is the purpose of adding weak acid, such as acetic acid, during the preparation of thermo-stable protein dispersions?
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What is the benefit of preparing thermo-stable protein dispersions from fishery products?
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What is the purpose of flocculation in protein recovery from process effluents?
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What is the benefit of enzymatically hydrolyzed proteins (FPHs) as functional additives?
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What is the role of proteolytic enzymes in extracting proteins from seafood processing discards?
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What is the benefit of concentrating proteins from process effluents using electroflocculation (EF) and ultrafiltration (UF)?
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What is the benefit of enzymatic treatment for extracting proteins from seafood processing discards?
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What is the role of mild heating to 50°C in the preparation of thermo-stable protein dispersions?
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What is the significance of the properties of enzymatically hydrolyzed proteins (FPHs)?
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What is the benefit of recovering proteins from by-catch fish?
What is the benefit of recovering proteins from by-catch fish?
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What is the main purpose of bioconversion processes in seafood processing?
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What is the approximate percentage of proteins in seafood processing discards?
What is the approximate percentage of proteins in seafood processing discards?
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What is the purpose of isoelectric solubilization precipitation (ISP) process?
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What is the benefit of using bioprocesses for protein recovery?
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What is the importance of focusing on bioavailability, interaction with other ingredients, nutritional, biotechnological, and sensorial aspects of seafood-derived compounds?
What is the importance of focusing on bioavailability, interaction with other ingredients, nutritional, biotechnological, and sensorial aspects of seafood-derived compounds?
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What is the role of high intensity sonication, electro-flocculation, and ultrafiltration in the ISP process?
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What is the main advantage of using seafood-derived compounds?
What is the main advantage of using seafood-derived compounds?
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What is the purpose of gelatin in food processing?
What is the purpose of gelatin in food processing?
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What is the main component of the nitrogenous fraction of seafood processing discards?
What is the main component of the nitrogenous fraction of seafood processing discards?
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What is the benefit of using chitosan and its oligosaccharides?
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Study Notes
Seafood Processing and Waste Generation
- Seafood industry generates large volumes of waste, including processing discards, effluents, and by-catch.
- Processing discards consist of shell, head, bones, intestine, fin, skin, and low-value underutilized fish.
- By-catch refers to the portion of the total harvest not adequately used as food due to unattractive color, smaller size, and high fat content.
Characteristics of Seafood Waste
- Solid waste constitutes 50% of whole shellfish, such as shrimp, krill, and crab.
- Shrimp waste contains about 70% head and 30% shell.
- Lobster processing generates 50-70% of the shellfish as by-products, including heads, shells, livers, and eggs.
- Finfish processing generates 25-50% of the raw material as waste.
Nutrient Loss and Environmental Impacts
- Seafood waste is rich in nutrients, including proteins, amino acids, lipids, carotenoids, and minerals.
- Loss of nutrients in seafood waste is estimated to be around 208 billion grams of protein and 1.8 trillion milligrams of n-3 PUFA per year.
- Environmental impacts of seafood waste include ocean dumping, reduced oxygen levels, burial or smothering of living organisms, and introduction of disease to the ecosystem.
Measures for Sustainable Seafood Processing
- Improve waste utilization to prevent or minimize environmental impact.
- Implement selective trawling to reduce by-catches.
- Treat wastes and effluents to reduce environmental hazards.
- Valorize wastes by recovering useful ingredients.
Conventional Processes for Waste Valorization and Their Limitations
- Conventional processes for valorization of seafood discards and effluents are based on chemical and physical methods.
- These processes have limitations, including:
- Alkali treatment causes hydrolysis of chitin and partial deacetylation of chitin.
- Chemical refining of fish oil can cause oxidation of unsaturated fatty acids.
- Large volumes of fresh water are required for washing and processing.
Biological Processes for Valorization
- Biological processes offer a green and sustainable approach to valorize seafood waste.
- Bioconversion reactions, including microbial fermentation and enzymatic processes, can be used to detach food components from their matrices.
- Algal biotechnology and bio-refinery approaches can be used to recover valuable ingredients and biofuels.
- Biological processes have minimal environmental impacts, are cost-effective, and are safe.### Algae-Induced Biotransformations
- Help in the treatment of wastewater and production of nutrient-rich biomass
- Biophysical processes like pH and temperature modifications can favor the release of components like proteins and lipids from food matrices
Microbe-Mediated Bioconversions
- Also known as fermentation, which is safe, environmentally friendly, and energy-efficient
- Process uses live microorganisms (bacteria, fungi, mycelium, or microalgae) to convert raw materials into products with desired qualities
- Types of fermentation: traditional, biomass, and precision fermentation
- Fermentation can be used to produce cell mass, enzymes, flavors, food additives, and other high-value products
- Lactic acid bacteria (LAB) have been used for a long time for the development of fermented fishery products
- Fermentation can bring about diversity into foods, make otherwise inedible foods products edible, enhance nutritional value, decrease toxicity, preserve food, and decrease cooking time and energy requirements
Microbe-Assisted Bioconversions
- Ideal for bioremediation of seafood processing waste and production of aquafeed and fertilizer
- Fermentation of fish by-products gives rise to quality protein hydrolysates and oil, and produces antioxidant compounds
- Solid-state fermentation can convert fish waste into a highly nutritive fertilizer
Algae-Based Bioconversions
- Microalgae such as Chlorella, Spirulina, Dunaliella, diatoms, and cyanobacteria are commonly used as sources of functional materials in natural foods
- These organisms have a high growth rate in nutrient media under phototrophic conditions
- They can be cultivated in open ponds or in closed photo-bioreactors, or heterotrophically in closed systems
- Microalgae are promising agents for bioconversions of food, fishery, and agricultural waste into biomass rich in bioactive compounds
Enzyme-Assisted Bioconversions
- Processing with enzymes has enormous potential in waste management
- Enzymes can mitigate hazards of conventional chemical transformations, help produce novel compounds, and function as analytical tools for food quality assessment
- Enzymes can be used as additional processing aids to conventional processes or can be exclusively used to upgrade existing technologies in seafood processing
- Examples of enzymes used in biotechnology include carbohydrases, proteases, and lipases
Downstream Processing for the Recovery of Seafood Components
-
The by-catch and the various seafood processing discards are rich in balanced proteins, collagen, enzymes, lipid, carotenoids, polysaccharides, and minerals
-
These can be recovered by coupling the various bioconversion processes, supported by marine biotechnology-based downstream processes
-
Examples of downstream processes include pressurized liquid, sub-critical, super-critical, enzymemediated, microwave-, and ultrasound-assisted extractions### Seafood Processing Discards as a Source of Proteins
-
Seafood processing discards contain up to 60% proteins, making them a good source of proteins.
-
Proteins from seafood processing discards can be recovered using bioprocessing techniques.
Isoelectric Solubilization Precipitation (ISP)
- ISP is a gentle bioprocess that involves homogenization of fish or processing discards with dilute acid or alkali.
- The process dissolves sarcoplasmic and myofibrillar proteins, while removing impurities such as bone, skin, oil, and membranes.
- Up to 90% of dissolved proteins can be precipitated by raising the pH of the solution to their iso-electric pH.
- The process can be enhanced by coupling with high intensity sonication, electro-flocculation, and ultrafiltration.
Recovery of Proteins from Various Fish Species
- Proteins have been recovered from various fish species, including mackerel, catfish, rockfish, Pacific whiting, rainbow trout, and Atlantic croaker.
- Fish protein isolates (FPIs) have protein contents of at least 65% and fat below 2%.
- FPIs differ from conventional surimi, which is a concentrate of fish myofibrillar proteins.
Properties of Fish Protein Isolates (FPIs)
- FPIs retain biochemical, nutritional, and functional properties of the native proteins.
- They can be used in applications such as development of restructured food products, protein supplements, and bioactive peptides.
Collagen and Collagen Hydrolysate (CH)
- Collagen is another protein from marine sources that can be recovered from seafood processing discards.
- Collagen-based novel functional food ingredients contain nutritional benefits, such as essential and non-essential amino acids.
- Collagen and CH can be used as natural antioxidants and texturizing agents.
Mild Acid Induced Gelation
- This process involves the ability of muscle structural proteins to undergo gelation under mild acidic conditions when water is strongly bound to the protein matrices.
- The process can be used to prepare thermo-stable protein dispersions from fishery products.
Flocculation
- Proteins can be flocculated and precipitated by food-grade polysaccharides, such as carrageenan, alginate, and carboxy methylcellulose.
- The proteins can be recovered from process effluents using electroflocculation and ultrafiltration.
Enzymatically Hydrolyzed Proteins
- Proteolytic enzymes from various sources can be used to extract proteins from seafood processing discards as fish protein hydrolyzates (FPHs).
- The ideal treatment conditions include incubation temperature, enzyme to substrate ratio, and incubation time.
- FPHs can be concentrated by spray drying or ultrafiltration and have beneficial effects on growth performances and feed utilization at low inclusion levels.
Seafood Processing and Waste Generation
- Seafood industry generates large volumes of waste, including processing discards, effluents, and by-catch.
- Processing discards consist of shell, head, bones, intestine, fin, skin, and low-value underutilized fish.
- By-catch refers to the portion of the total harvest not adequately used as food due to unattractive color, smaller size, and high fat content.
Characteristics of Seafood Waste
- Solid waste constitutes 50% of whole shellfish, such as shrimp, krill, and crab.
- Shrimp waste contains about 70% head and 30% shell.
- Lobster processing generates 50-70% of the shellfish as by-products, including heads, shells, livers, and eggs.
- Finfish processing generates 25-50% of the raw material as waste.
Nutrient Loss and Environmental Impacts
- Seafood waste is rich in nutrients, including proteins, amino acids, lipids, carotenoids, and minerals.
- Loss of nutrients in seafood waste is estimated to be around 208 billion grams of protein and 1.8 trillion milligrams of n-3 PUFA per year.
- Environmental impacts of seafood waste include ocean dumping, reduced oxygen levels, burial or smothering of living organisms, and introduction of disease to the ecosystem.
Measures for Sustainable Seafood Processing
- Improve waste utilization to prevent or minimize environmental impact.
- Implement selective trawling to reduce by-catches.
- Treat wastes and effluents to reduce environmental hazards.
- Valorize wastes by recovering useful ingredients.
Conventional Processes for Waste Valorization and Their Limitations
- Conventional processes for valorization of seafood discards and effluents are based on chemical and physical methods.
- These processes have limitations, including:
- Alkali treatment causes hydrolysis of chitin and partial deacetylation of chitin.
- Chemical refining of fish oil can cause oxidation of unsaturated fatty acids.
- Large volumes of fresh water are required for washing and processing.
Biological Processes for Valorization
- Biological processes offer a green and sustainable approach to valorize seafood waste.
- Bioconversion reactions, including microbial fermentation and enzymatic processes, can be used to detach food components from their matrices.
- Algal biotechnology and bio-refinery approaches can be used to recover valuable ingredients and biofuels.
- Biological processes have minimal environmental impacts, are cost-effective, and are safe.### Algae-Induced Biotransformations
- Help in the treatment of wastewater and production of nutrient-rich biomass
- Biophysical processes like pH and temperature modifications can favor the release of components like proteins and lipids from food matrices
Microbe-Mediated Bioconversions
- Also known as fermentation, which is safe, environmentally friendly, and energy-efficient
- Process uses live microorganisms (bacteria, fungi, mycelium, or microalgae) to convert raw materials into products with desired qualities
- Types of fermentation: traditional, biomass, and precision fermentation
- Fermentation can be used to produce cell mass, enzymes, flavors, food additives, and other high-value products
- Lactic acid bacteria (LAB) have been used for a long time for the development of fermented fishery products
- Fermentation can bring about diversity into foods, make otherwise inedible foods products edible, enhance nutritional value, decrease toxicity, preserve food, and decrease cooking time and energy requirements
Microbe-Assisted Bioconversions
- Ideal for bioremediation of seafood processing waste and production of aquafeed and fertilizer
- Fermentation of fish by-products gives rise to quality protein hydrolysates and oil, and produces antioxidant compounds
- Solid-state fermentation can convert fish waste into a highly nutritive fertilizer
Algae-Based Bioconversions
- Microalgae such as Chlorella, Spirulina, Dunaliella, diatoms, and cyanobacteria are commonly used as sources of functional materials in natural foods
- These organisms have a high growth rate in nutrient media under phototrophic conditions
- They can be cultivated in open ponds or in closed photo-bioreactors, or heterotrophically in closed systems
- Microalgae are promising agents for bioconversions of food, fishery, and agricultural waste into biomass rich in bioactive compounds
Enzyme-Assisted Bioconversions
- Processing with enzymes has enormous potential in waste management
- Enzymes can mitigate hazards of conventional chemical transformations, help produce novel compounds, and function as analytical tools for food quality assessment
- Enzymes can be used as additional processing aids to conventional processes or can be exclusively used to upgrade existing technologies in seafood processing
- Examples of enzymes used in biotechnology include carbohydrases, proteases, and lipases
Downstream Processing for the Recovery of Seafood Components
-
The by-catch and the various seafood processing discards are rich in balanced proteins, collagen, enzymes, lipid, carotenoids, polysaccharides, and minerals
-
These can be recovered by coupling the various bioconversion processes, supported by marine biotechnology-based downstream processes
-
Examples of downstream processes include pressurized liquid, sub-critical, super-critical, enzymemediated, microwave-, and ultrasound-assisted extractions### Seafood Processing Discards as a Source of Proteins
-
Seafood processing discards contain up to 60% proteins, making them a good source of proteins.
-
Proteins from seafood processing discards can be recovered using bioprocessing techniques.
Isoelectric Solubilization Precipitation (ISP)
- ISP is a gentle bioprocess that involves homogenization of fish or processing discards with dilute acid or alkali.
- The process dissolves sarcoplasmic and myofibrillar proteins, while removing impurities such as bone, skin, oil, and membranes.
- Up to 90% of dissolved proteins can be precipitated by raising the pH of the solution to their iso-electric pH.
- The process can be enhanced by coupling with high intensity sonication, electro-flocculation, and ultrafiltration.
Recovery of Proteins from Various Fish Species
- Proteins have been recovered from various fish species, including mackerel, catfish, rockfish, Pacific whiting, rainbow trout, and Atlantic croaker.
- Fish protein isolates (FPIs) have protein contents of at least 65% and fat below 2%.
- FPIs differ from conventional surimi, which is a concentrate of fish myofibrillar proteins.
Properties of Fish Protein Isolates (FPIs)
- FPIs retain biochemical, nutritional, and functional properties of the native proteins.
- They can be used in applications such as development of restructured food products, protein supplements, and bioactive peptides.
Collagen and Collagen Hydrolysate (CH)
- Collagen is another protein from marine sources that can be recovered from seafood processing discards.
- Collagen-based novel functional food ingredients contain nutritional benefits, such as essential and non-essential amino acids.
- Collagen and CH can be used as natural antioxidants and texturizing agents.
Mild Acid Induced Gelation
- This process involves the ability of muscle structural proteins to undergo gelation under mild acidic conditions when water is strongly bound to the protein matrices.
- The process can be used to prepare thermo-stable protein dispersions from fishery products.
Flocculation
- Proteins can be flocculated and precipitated by food-grade polysaccharides, such as carrageenan, alginate, and carboxy methylcellulose.
- The proteins can be recovered from process effluents using electroflocculation and ultrafiltration.
Enzymatically Hydrolyzed Proteins
- Proteolytic enzymes from various sources can be used to extract proteins from seafood processing discards as fish protein hydrolyzates (FPHs).
- The ideal treatment conditions include incubation temperature, enzyme to substrate ratio, and incubation time.
- FPHs can be concentrated by spray drying or ultrafiltration and have beneficial effects on growth performances and feed utilization at low inclusion levels.
Seafood Processing and Waste Generation
- Seafood industry generates large volumes of waste, including processing discards, effluents, and by-catch.
- Processing discards consist of shell, head, bones, intestine, fin, skin, and low-value underutilized fish.
- By-catch refers to the portion of the total harvest not adequately used as food due to unattractive color, smaller size, and high fat content.
Characteristics of Seafood Waste
- Solid waste constitutes 50% of whole shellfish, such as shrimp, krill, and crab.
- Shrimp waste contains about 70% head and 30% shell.
- Lobster processing generates 50-70% of the shellfish as by-products, including heads, shells, livers, and eggs.
- Finfish processing generates 25-50% of the raw material as waste.
Nutrient Loss and Environmental Impacts
- Seafood waste is rich in nutrients, including proteins, amino acids, lipids, carotenoids, and minerals.
- Loss of nutrients in seafood waste is estimated to be around 208 billion grams of protein and 1.8 trillion milligrams of n-3 PUFA per year.
- Environmental impacts of seafood waste include ocean dumping, reduced oxygen levels, burial or smothering of living organisms, and introduction of disease to the ecosystem.
Measures for Sustainable Seafood Processing
- Improve waste utilization to prevent or minimize environmental impact.
- Implement selective trawling to reduce by-catches.
- Treat wastes and effluents to reduce environmental hazards.
- Valorize wastes by recovering useful ingredients.
Conventional Processes for Waste Valorization and Their Limitations
- Conventional processes for valorization of seafood discards and effluents are based on chemical and physical methods.
- These processes have limitations, including:
- Alkali treatment causes hydrolysis of chitin and partial deacetylation of chitin.
- Chemical refining of fish oil can cause oxidation of unsaturated fatty acids.
- Large volumes of fresh water are required for washing and processing.
Biological Processes for Valorization
- Biological processes offer a green and sustainable approach to valorize seafood waste.
- Bioconversion reactions, including microbial fermentation and enzymatic processes, can be used to detach food components from their matrices.
- Algal biotechnology and bio-refinery approaches can be used to recover valuable ingredients and biofuels.
- Biological processes have minimal environmental impacts, are cost-effective, and are safe.### Algae-Induced Biotransformations
- Help in the treatment of wastewater and production of nutrient-rich biomass
- Biophysical processes like pH and temperature modifications can favor the release of components like proteins and lipids from food matrices
Microbe-Mediated Bioconversions
- Also known as fermentation, which is safe, environmentally friendly, and energy-efficient
- Process uses live microorganisms (bacteria, fungi, mycelium, or microalgae) to convert raw materials into products with desired qualities
- Types of fermentation: traditional, biomass, and precision fermentation
- Fermentation can be used to produce cell mass, enzymes, flavors, food additives, and other high-value products
- Lactic acid bacteria (LAB) have been used for a long time for the development of fermented fishery products
- Fermentation can bring about diversity into foods, make otherwise inedible foods products edible, enhance nutritional value, decrease toxicity, preserve food, and decrease cooking time and energy requirements
Microbe-Assisted Bioconversions
- Ideal for bioremediation of seafood processing waste and production of aquafeed and fertilizer
- Fermentation of fish by-products gives rise to quality protein hydrolysates and oil, and produces antioxidant compounds
- Solid-state fermentation can convert fish waste into a highly nutritive fertilizer
Algae-Based Bioconversions
- Microalgae such as Chlorella, Spirulina, Dunaliella, diatoms, and cyanobacteria are commonly used as sources of functional materials in natural foods
- These organisms have a high growth rate in nutrient media under phototrophic conditions
- They can be cultivated in open ponds or in closed photo-bioreactors, or heterotrophically in closed systems
- Microalgae are promising agents for bioconversions of food, fishery, and agricultural waste into biomass rich in bioactive compounds
Enzyme-Assisted Bioconversions
- Processing with enzymes has enormous potential in waste management
- Enzymes can mitigate hazards of conventional chemical transformations, help produce novel compounds, and function as analytical tools for food quality assessment
- Enzymes can be used as additional processing aids to conventional processes or can be exclusively used to upgrade existing technologies in seafood processing
- Examples of enzymes used in biotechnology include carbohydrases, proteases, and lipases
Downstream Processing for the Recovery of Seafood Components
-
The by-catch and the various seafood processing discards are rich in balanced proteins, collagen, enzymes, lipid, carotenoids, polysaccharides, and minerals
-
These can be recovered by coupling the various bioconversion processes, supported by marine biotechnology-based downstream processes
-
Examples of downstream processes include pressurized liquid, sub-critical, super-critical, enzymemediated, microwave-, and ultrasound-assisted extractions### Seafood Processing Discards as a Source of Proteins
-
Seafood processing discards contain up to 60% proteins, making them a good source of proteins.
-
Proteins from seafood processing discards can be recovered using bioprocessing techniques.
Isoelectric Solubilization Precipitation (ISP)
- ISP is a gentle bioprocess that involves homogenization of fish or processing discards with dilute acid or alkali.
- The process dissolves sarcoplasmic and myofibrillar proteins, while removing impurities such as bone, skin, oil, and membranes.
- Up to 90% of dissolved proteins can be precipitated by raising the pH of the solution to their iso-electric pH.
- The process can be enhanced by coupling with high intensity sonication, electro-flocculation, and ultrafiltration.
Recovery of Proteins from Various Fish Species
- Proteins have been recovered from various fish species, including mackerel, catfish, rockfish, Pacific whiting, rainbow trout, and Atlantic croaker.
- Fish protein isolates (FPIs) have protein contents of at least 65% and fat below 2%.
- FPIs differ from conventional surimi, which is a concentrate of fish myofibrillar proteins.
Properties of Fish Protein Isolates (FPIs)
- FPIs retain biochemical, nutritional, and functional properties of the native proteins.
- They can be used in applications such as development of restructured food products, protein supplements, and bioactive peptides.
Collagen and Collagen Hydrolysate (CH)
- Collagen is another protein from marine sources that can be recovered from seafood processing discards.
- Collagen-based novel functional food ingredients contain nutritional benefits, such as essential and non-essential amino acids.
- Collagen and CH can be used as natural antioxidants and texturizing agents.
Mild Acid Induced Gelation
- This process involves the ability of muscle structural proteins to undergo gelation under mild acidic conditions when water is strongly bound to the protein matrices.
- The process can be used to prepare thermo-stable protein dispersions from fishery products.
Flocculation
- Proteins can be flocculated and precipitated by food-grade polysaccharides, such as carrageenan, alginate, and carboxy methylcellulose.
- The proteins can be recovered from process effluents using electroflocculation and ultrafiltration.
Enzymatically Hydrolyzed Proteins
- Proteolytic enzymes from various sources can be used to extract proteins from seafood processing discards as fish protein hydrolyzates (FPHs).
- The ideal treatment conditions include incubation temperature, enzyme to substrate ratio, and incubation time.
- FPHs can be concentrated by spray drying or ultrafiltration and have beneficial effects on growth performances and feed utilization at low inclusion levels.
Fisheries and Aquaculture Production
- The total world fisheries and aquaculture production showed a 41% growth from 2000 to 2019, reaching 178 million tons in 2019.
- Molluscs comprised 13% of the total production, while crustaceans comprised 9%.
Shell Waste Generation and Composition
- Close to 8 million tons of waste crab, shrimp, and lobster shells are produced globally each year, comprising up to 60% of overall crab mass.
- Over 10 million tons of mollusc shells are produced annually, with over 70% represented by oyster, clam, scallop, and mussel shells.
- Shell waste accounts for 65-90% of live weight, depending on the species.
- Mussels have the greatest increase in production and shell contribution compared to other shellfish species.
Shell Waste Disposal and Environmental Impacts
- Disposed shells are primarily dumped at sea or sent to landfills, causing environmental damage.
- Shell waste can lead to decomposition of residual tissues, foul odors, and visual pollution.
- Shell waste is generated at various locations, including fisheries, aquaculture, processing companies, restaurants, and individual consumers.
Chemical Composition of Shells
- Shells are composed mainly of calcium carbonate (CaCO3).
- The decomposition of shells requires high temperatures of over 1000°C, resulting in high energy consumption and greenhouse gas emissions.
Valorization of Shell Waste
- Shell waste can be valorized through various applications, including agriculture, medicine, chemical production, construction, environmental protection, and cosmetics.
- The valorization of shells and the unlocking of markets for new products is essential for sustainable waste management.
Cleaning and Preparation of Shells
- Shells need to be cleaned prior to industrial use.
- Cleaning methods include washing with freshwater, heating at 500°C to burn off residual organic material, and enzymatic cleaning.
Environmental Applications of Shell Waste
- Shell waste can be used for water purification and bio-filters, particularly in wastewater treatment and phosphate removal.
- Calcined shells can be used for heavy metal removal from water and wastewater.
- Shell waste can be used for soil remediation, acid mine drainage treatment, and ocean deacidification.
Sustainability Challenges
- Cleaning shells with freshwater is water-intensive, and the use of enzymes is costly.
- The calcination of shells requires high temperatures, resulting in energy consumption and greenhouse gas emissions.### Sensitivity to Ocean Acidification
- Shellfish are more sensitive to ocean acidification at the larval stage than at the adult stage
- Ocean acidification can negatively impact shellfish production
- Increasing seawater alkalinity can help mitigate the effects of ocean acidification
- Restoration of macroalgae and seagrass beds can remove CO2 from seawater during photosynthesis
Use of Waste Shells
- Waste shells can be used to buffer decreases in seawater carbonate due to ocean acidification
- Placing shell reefs adjacent to shellfish farms can improve pH and carbonate geochemical conditions
- Waste shells can control benthic acidity, prevent heavy metal speciation, and reduce eutrophication
- Waste shells can serve as settlement substrates for free-living shellfish larvae and as shelter for other aquatic organisms
Flue Gas Treatment
- Calcined shell waste can be used as an excellent biosorbent material to remove pollutants like SO2, SO3, H2S, and NOx
- Calcined oyster shells can be used to prepare CaO adsorbent for CO2 sequestration
Road De-icing
- Crushed freshwater bivalve shells can be used as an alternative to sodium chloride (NaCl) for road de-icing
- Calcium-magnesium acetate or calcium acetate derivatives can be used as environmentally friendly road de-icers
Green Roofing
- Mollusc shells can be used as a material for green roofing structures, such as drainage layers, or to help neutralize acid rain
- Shells have bioremediation potential and can be used to reduce runoff water
Sustainability Challenges
- Calcination of waste shells requires high temperatures and special machinery, making it a non-sustainable solution
- Using raw shells as bio-filters can be a more sustainable solution, but requires careful consideration of instability in acidic waters and sludge formation
Food and Feed Additives; Nutraceuticals
- Waste shells can be used to produce natural shell powder, a relevant source of calcium for the food and nutraceutical industry
- Chitin is widely used to immobilize enzymes and whole cells in the food industry
- Chitosan is used as a preservative, thickener, and stabilizer in food products
Calcium Supplement
- Calcium supplementation from shell powders can improve bone health, blood circulation, and eggshell quality in livestock
Protein Supplement
- Meals derived from crustacean shell waste are usable feed ingredients, rich in essential amino acids
- Shell fermentation products can be used as a feed ingredient in the aquaculture industry
Antibacterials for Food
- Macromolecular chitosan has powerful antibacterial properties, inhibiting bacterial growth on surfaces of aquatic and meat products
Bioactive Molecules/Antioxidants
- Chitosan and chitosan oligosaccharides act as potent antioxidants, scavenging free radicals and preventing oxidative sequences
Bioactive Molecules/Cholesterol Reduction
- Chitosan acts as a hypocholesterolemic agent, reducing LDL-cholesterol levels and increasing HDL-cholesterol levels
Bioactive Molecules/Blood Pressure Reduction
- Chitosan oligosaccharides have angiotensin-converting-enzyme (ACE) inhibitory activity, reducing peripheral blood pressure and having an anti-hypertensive effect
Sustainability Challenges
- Extracting chemicals from waste crustacean shells is destructive and expensive, requiring fractionation and the use of hazardous chemicals
- A sustainable fractionation method to separate proteins, CaCO3, and chitin without hazardous chemicals is needed
Biomaterials
- Shells of both crustaceans and molluscs have potential applications in biofuels, flavors, fragrances, pharmaceuticals, industrial chemicals, and biomaterials
- Chitin and chitosan can be used as chemical building blocks for various biomedical applications
Bone Tissue Regeneration/Bioceramic
- Powdered oyster nacre can stimulate bone cell differentiation and bone formation in vitro and in vivo
- Oyster shell powder can be used as an injectable osteogenic biomaterial for treating vertebral bone loss
Cosmetics
- Clam shell powder has potential as a new biomaterial with high calcium, phosphate, chitin, and protein content
- Hydroxyapatite from shells can be used as an additive in sunscreen lotions, substituting oxybenzone, zinc oxide, and titanium dioxide
Wound Healing
- Chitin film and fiber can be used as a wound-dressing material, exhibiting bacteriostatic and fungistatic properties
Biocomposites/Biofillers
- Calcined, powdered mollusc shells can be used as additives for polyesters, endowing antibacterial properties to biocomposites
Biopackaging
-
Biopolymers deriving from waste shells can be used to manufacture eco-friendly bioplastics for biomedical, industrial, and household applications### Antimicrobial Biopackaging
-
Chitosan-based biopackaging is a promising active packaging type developed in the last decade
-
Chitosan is easier to process than chitin, but its stability is lower due to its hydrophilic character and pH sensitivity
Sustainability Challenges
- Many potential applications for shell waste as biomaterials are only developed as niche applications or tested at laboratory scale
- Further investigation is needed to develop new separation and enrichment technologies, and to stabilize bioactive molecules
- The production of hydroxyapatite from waste shells is an example of a sustainable and industrially advantageous process
Construction
- Shells have been used in construction as path aggregates, mortar mixes, and as a replacement for conventional aggregates in concrete
- Mollusc shells can be used as cement additives or aggregates, improving the elasticity and bonding between the binder matrix and aggregates
- Waste shell powder can supplement non-renewable limestone powder in cement-based materials and can be modified to mitigate noise pollution
- The use of waste shells in construction helps with waste management and produces cost-efficient, green concrete
Construction Applications
- Waste shells can be used to produce gypsum plaster or green cement, which is used mainly in interior applications
- Shells can be incorporated as asphalt modifiers, improving its consistency, hardness, and elasticity
- The utilization of waste shells in concrete reduces the environmental pressure, damage, and shortages due to excessive quarrying and limestone extraction
Sustainability Challenges in Construction
- The pretreatment of waste shells requires washing and calcination, which uses significant amounts of resources and energy
- High chloride content in aggregates can cause faster corrosion of steel, and its use should be avoided in reinforced concrete
- The cost of shell cleaning may be higher than in conventional aggregates, and the transportation of additional quantities of shells may increase its overall price
- The quantity of waste shells available may not be sustainable enough as an aggregate replacement material
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Description
Learn about the types of waste generated by the seafood industry, including processing discards, effluents, and by-catch, and their characteristics.