parasitology laboratory
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Questions and Answers

How long is stage micrometer?

1000 um

What parasites can be found in sputum?

Paragonimus westermani Motile larvae Entamoeba gingivalis Entamoeba histolytica Strongyloides stercoralis Hookworms Ascaris lumbricoides

What specialized stain is used to detect oocysts of Cryptosporidium and Isospora and Cyclospora?

Modified acid fast stain

What color is the when oocysts of Crypstosporidium ad Isospora stained?

<p>Pink to red</p> Signup and view all the answers

From bottom to top, what are the resulting layers in floatation technique?

<p>Sediment Formalin Debris Ethyl acetate</p> Signup and view all the answers

What nematodes are effective in floatation method?

<p>Enterobius vermicularis Trichuris trichiura Hookworm</p> Signup and view all the answers

What are the parasites that will sink to the bottom in floatation technique?

<p>Schistosoma spp. Ascaris lumbricoides Blastocyctis spp.</p> Signup and view all the answers

What is the specific gravity of zinc sulfate?

<p>1.18-1.20</p> Signup and view all the answers

What is the actual measurement of the parasite if: ocular micrometer = 0.51 Stage micrometer = 0.12 mm 3 ocular units

<p>7.05 um</p> Signup and view all the answers

Study Notes

Microscopy and Parasite Detection

  • A stage micrometer is 0.12 mm in length.

Parasites in Sputum

  • Various parasites can be found in sputum, including oocysts of Cryptosporidium, Isospora, and Cyclospora.

Staining Techniques

  • A specialized stain, such as Modified Acid-Fast Stain, is used to detect oocysts of Cryptosporidium, Isospora, and Cyclospora.
  • Oocysts of Cryptosporidium and Isospora stain red or pink when using Modified Acid-Fast Stain.

Floatation Technique

  • The resulting layers from bottom to top in the floatation technique are: sediment, formalin, and zinc sulfate.
  • Nematodes, such as Trichuris and Ascaris, are effective in the floatation method.
  • Protozoa, such as Giardia and Entamoeba, and helminth eggs, such as hookworms, will sink to the bottom in the floatation technique.

Floatation Solution

  • The specific gravity of zinc sulfate is 1.18.

Measurement Conversions

  • To calculate the actual measurement of a parasite, multiply the ocular units by the conversion factor: 3 ocular units × (0.12 mm / 0.51) = actual measurement.

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