9 Questions
How long is stage micrometer?
1000 um
What parasites can be found in sputum?
Paragonimus westermani Motile larvae Entamoeba gingivalis Entamoeba histolytica Strongyloides stercoralis Hookworms Ascaris lumbricoides
What specialized stain is used to detect oocysts of Cryptosporidium and Isospora and Cyclospora?
Modified acid fast stain
What color is the when oocysts of Crypstosporidium ad Isospora stained?
Pink to red
From bottom to top, what are the resulting layers in floatation technique?
Sediment Formalin Debris Ethyl acetate
What nematodes are effective in floatation method?
Enterobius vermicularis Trichuris trichiura Hookworm
What are the parasites that will sink to the bottom in floatation technique?
Schistosoma spp. Ascaris lumbricoides Blastocyctis spp.
What is the specific gravity of zinc sulfate?
1.18-1.20
What is the actual measurement of the parasite if: ocular micrometer = 0.51 Stage micrometer = 0.12 mm 3 ocular units
7.05 um
Study Notes
Microscopy and Parasite Detection
- A stage micrometer is 0.12 mm in length.
Parasites in Sputum
- Various parasites can be found in sputum, including oocysts of Cryptosporidium, Isospora, and Cyclospora.
Staining Techniques
- A specialized stain, such as Modified Acid-Fast Stain, is used to detect oocysts of Cryptosporidium, Isospora, and Cyclospora.
- Oocysts of Cryptosporidium and Isospora stain red or pink when using Modified Acid-Fast Stain.
Floatation Technique
- The resulting layers from bottom to top in the floatation technique are: sediment, formalin, and zinc sulfate.
- Nematodes, such as Trichuris and Ascaris, are effective in the floatation method.
- Protozoa, such as Giardia and Entamoeba, and helminth eggs, such as hookworms, will sink to the bottom in the floatation technique.
Floatation Solution
- The specific gravity of zinc sulfate is 1.18.
Measurement Conversions
- To calculate the actual measurement of a parasite, multiply the ocular units by the conversion factor: 3 ocular units × (0.12 mm / 0.51) = actual measurement.
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