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Questions and Answers
Which region of an antibody recognizes and binds to other proteins?
What is the part of a protein that an antibody recognizes called?
Which region of an antibody remains constant for every antibody within a species?
Why are antibodies useful as detection reagents?
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Which term refers to the specific part of an antigen that an antibody recognizes?
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What is the purpose of adding a second antibody with a label or dye in an ELISA?
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What is the role of the enzyme in an ELISA?
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What does the intensity of the color in an ELISA well indicate?
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Which of the following is true about protein-based diagnostics?
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What is a biomarker?
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Which protein is used as an indicator of inflammation?
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What are antibodies?
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Which type of ELISA allows for the detection of multiple antigens simultaneously?
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What is the purpose of attaching antibodies to beads in a multiplexed ELISA?
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What is the advantage of using multi-well plates in a multiplexed ELISA?
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Why is evaporation a concern in 1536 well plates in a multiplexed ELISA?
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Which layer of the Multiplexed Bead Assays is responsible for capturing the antigen on the bead?
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What is the purpose of the second antibody in the Multiplexed Bead Assays?
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What is the purpose of the discriminator signal in the Multiplexed Bead Assays?
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What is the purpose of flow cytometry in the Multiplexed Bead Assays?
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Which of the following is NOT a physio-chemical characteristic used in protein chromatography?
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What is the purpose of the positive control in a pregnancy test?
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What happens if there is no color change in the control zone of a pregnancy test?
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What is the purpose of protein chromatography?
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Which type of matrix is used in ion exchange chromatography?
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Which type of matrix is used in affinity chromatography?
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What is the stationary phase in column chromatography?
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What is the mobile phase in column chromatography?
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What type of matrix is used in size exclusion chromatography?
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What carries the protein mixture in column chromatography?
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Which part of an antibody remains constant for every antibody within a species?
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What is the specific part of a protein that an antibody recognizes called?
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What is the purpose of attaching antibodies to beads in a multiplexed ELISA?
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What happens if there is no color change in the control zone of a pregnancy test?
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Which of the following is true about ELISA?
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What is the purpose of the second antibody in an ELISA?
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What is the role of the enzyme in an ELISA?
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What does the intensity of the color in an ELISA well indicate?
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Which of the following is NOT a protein-based diagnostic technology discussed in this course?
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What is the purpose of using antibodies in protein-based diagnostics?
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Which protein is mentioned as an example of a biomarker in protein-based diagnostics?
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What are antibodies?
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Which type of plate reader can analyze different plate types with different numbers of wells?
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What is a concern when using 1536 well plates in a multiplexed ELISA?
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What is the purpose of using robots in automated multiplexed assays?
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What is the role of the dye in the inert material of the bead in a multiplexed assay?
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Which of the following is NOT a physio-chemical characteristic used in protein chromatography?
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What is the purpose of the control zone in a pregnancy test?
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What is the purpose of the positive control in a protein-based diagnostic test?
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What is the purpose of the second antibody in the Multiplexed Bead Assays?
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Which of the following is true about the Multiplexed Bead Assays?
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What is the purpose of the second antibody in the home pregnancy test?
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What is the term for the specific part of an antigen that an antibody recognizes?
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What is the purpose of flow cytometry in the Multiplexed Bead Assays?
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Which type of matrix is used in ion exchange chromatography?
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What is the purpose of the stationary phase in column chromatography?
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What happens if there is no color change in the control zone of a pregnancy test?
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Which region of an antibody remains constant for every antibody within a species?
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What is the purpose of attaching a particular molecule to the matrix in affinity chromatography?
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What is the purpose of the mobile phase in column chromatography?
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