Podcast
Questions and Answers
Which dye is commonly used in real-time PCR as an intercalating dye?
Which dye is commonly used in real-time PCR as an intercalating dye?
- Fluorescein
- FAM
- Quencher
- SYBR green (correct)
What happens to the fluorescence level in the hydrolysis probe technique during PCR?
What happens to the fluorescence level in the hydrolysis probe technique during PCR?
- It fluctuates
- It remains constant
- It decreases
- It increases (correct)
What is the purpose of using two sequence-specific oligonucleotide probes in detection method 3?
What is the purpose of using two sequence-specific oligonucleotide probes in detection method 3?
- To generate fluorescence during PCR
- To label the DNA double helix
- To increase the sensitivity of the PCR (correct)
- To assess the melting point of DNA
What is the purpose of melting curve analysis in real-time PCR?
What is the purpose of melting curve analysis in real-time PCR?
Which method can be used to separate DNA from RNA and protein based on differences in buoyant density?
Which method can be used to separate DNA from RNA and protein based on differences in buoyant density?
What is the purpose of cell lysis in the isolation of nucleic acids?
What is the purpose of cell lysis in the isolation of nucleic acids?
Which of the following methods is commonly used for DNA amplification?
Which of the following methods is commonly used for DNA amplification?
What is the function of plasmids?
What is the function of plasmids?
Which of the following is a key application of PCR in forensic science?
Which of the following is a key application of PCR in forensic science?
What is the purpose of annealing in a PCR cycle?
What is the purpose of annealing in a PCR cycle?
What is the typical length of PCR primers?
What is the typical length of PCR primers?
What is the purpose of real-time PCR?
What is the purpose of real-time PCR?
Who coined the term 'plasmid' and what did he win the Nobel Prize for?
Who coined the term 'plasmid' and what did he win the Nobel Prize for?
What is the main characteristic of plasmids?
What is the main characteristic of plasmids?
What is the normal number of copies of plasmid found in a single cell called?
What is the normal number of copies of plasmid found in a single cell called?
What is the purpose of using phenol and chloroform in DNA/RNA purification?
What is the purpose of using phenol and chloroform in DNA/RNA purification?
Which enzyme is added to the pyrosequencing reaction mixture to catalyze the incorporation of nucleotides?
Which enzyme is added to the pyrosequencing reaction mixture to catalyze the incorporation of nucleotides?
What is the limitation of pyrosequencing when it comes to detecting homopolymeric regions?
What is the limitation of pyrosequencing when it comes to detecting homopolymeric regions?
Which enzyme catalyzes the reaction of pyrophosphate (PPi) with adenosine 5’ phosphosulfate (APS) in pyrosequencing?
Which enzyme catalyzes the reaction of pyrophosphate (PPi) with adenosine 5’ phosphosulfate (APS) in pyrosequencing?
What triggers the release of visible light in pyrosequencing?
What triggers the release of visible light in pyrosequencing?
Which enzyme does the virus use to produce DNA from its RNA genome?
Which enzyme does the virus use to produce DNA from its RNA genome?
What is the purpose of the Ct value in real-time PCR?
What is the purpose of the Ct value in real-time PCR?
What is the formula to calculate fold changes in gene expression using qRT-PCR?
What is the formula to calculate fold changes in gene expression using qRT-PCR?
What is the disadvantage of qPCR?
What is the disadvantage of qPCR?
Which of the following is a step in RNA-seq library construction?
Which of the following is a step in RNA-seq library construction?
What is the purpose of using oligo(dT) beads in RNA-seq library construction?
What is the purpose of using oligo(dT) beads in RNA-seq library construction?
Which enzyme is used to degrade only the RNA in RNA:DNA hybrids during second strand synthesis in RNA-seq library construction?
Which enzyme is used to degrade only the RNA in RNA:DNA hybrids during second strand synthesis in RNA-seq library construction?
What is the purpose of using random hexamers instead of oligo(dT) primers for first strand synthesis in RNA-seq library construction?
What is the purpose of using random hexamers instead of oligo(dT) primers for first strand synthesis in RNA-seq library construction?
What is the purpose of phosphorylating the dsDNAs at their 5' ends in RNA-seq library construction?
What is the purpose of phosphorylating the dsDNAs at their 5' ends in RNA-seq library construction?
What is the sequence of the Y-shaped adapters used in Illumina library construction?
What is the sequence of the Y-shaped adapters used in Illumina library construction?
Which detection method in real-time PCR uses two sequence-specific oligonucleotide probes labeled with different dyes?
Which detection method in real-time PCR uses two sequence-specific oligonucleotide probes labeled with different dyes?
What is the purpose of melting curve analysis in real-time PCR?
What is the purpose of melting curve analysis in real-time PCR?
Which enzyme is responsible for reverse transcription in the synthesis of cDNA using single-stranded RNA as a template?
Which enzyme is responsible for reverse transcription in the synthesis of cDNA using single-stranded RNA as a template?
What is the genetic material used by retroviruses?
What is the genetic material used by retroviruses?
Which scientist coined the term 'plasmid' and won the Nobel Prize for discovering bacterial conjugation?
Which scientist coined the term 'plasmid' and won the Nobel Prize for discovering bacterial conjugation?
What is the main characteristic of plasmids?
What is the main characteristic of plasmids?
What is the purpose of using phenol and chloroform in DNA/RNA purification?
What is the purpose of using phenol and chloroform in DNA/RNA purification?
What is the purpose of real-time PCR?
What is the purpose of real-time PCR?
Which of the following is a key application of PCR in forensic science?
Which of the following is a key application of PCR in forensic science?
What is the purpose of the Ct value in real-time PCR?
What is the purpose of the Ct value in real-time PCR?
What is the limitation of pyrosequencing when it comes to detecting homopolymeric regions?
What is the limitation of pyrosequencing when it comes to detecting homopolymeric regions?
What is the purpose of melting curve analysis in real-time PCR?
What is the purpose of melting curve analysis in real-time PCR?
Which enzyme is responsible for catalyzing the reaction of ATP with the substrate luciferin in pyrosequencing?
Which enzyme is responsible for catalyzing the reaction of ATP with the substrate luciferin in pyrosequencing?
What is the limitation of pyrosequencing when it comes to detecting homopolymeric regions?
What is the limitation of pyrosequencing when it comes to detecting homopolymeric regions?
What triggers the release of pyrophosphate (PPi) in pyrosequencing?
What triggers the release of pyrophosphate (PPi) in pyrosequencing?
Which enzyme is used to degrade excess nucleotide dNTP and ATP in the pyrosequencing reaction mixture?
Which enzyme is used to degrade excess nucleotide dNTP and ATP in the pyrosequencing reaction mixture?
Which of the following is true about Ct values in real-time PCR?
Which of the following is true about Ct values in real-time PCR?
What is the purpose of a standard curve in absolute quantification of gene expression?
What is the purpose of a standard curve in absolute quantification of gene expression?
What is the main advantage of relative quantification over absolute quantification in measuring gene expression?
What is the main advantage of relative quantification over absolute quantification in measuring gene expression?
What is the formula to calculate fold changes in gene expression using qRT-PCR?
What is the formula to calculate fold changes in gene expression using qRT-PCR?
Which of the following is NOT a method used for cell lysis in the isolation of nucleic acids?
Which of the following is NOT a method used for cell lysis in the isolation of nucleic acids?
Which of the following correctly describes the separation of DNA, RNA, and protein by centrifugation in cesium chloride (CsCl) gradient?
Which of the following correctly describes the separation of DNA, RNA, and protein by centrifugation in cesium chloride (CsCl) gradient?
What is the purpose of centrifuging the CsCl gradient at very high speeds in the separation of DNA, RNA, and protein?
What is the purpose of centrifuging the CsCl gradient at very high speeds in the separation of DNA, RNA, and protein?
What are plasmids?
What are plasmids?
Which step is performed at 16°C during RNA-seq library construction?
Which step is performed at 16°C during RNA-seq library construction?
Which molecule is specifically degraded by Ribonuclease H during second strand synthesis in RNA-seq library construction?
Which molecule is specifically degraded by Ribonuclease H during second strand synthesis in RNA-seq library construction?
What is the purpose of adding an 'A' base to the 3' ends of dsDNAs in RNA-seq library construction?
What is the purpose of adding an 'A' base to the 3' ends of dsDNAs in RNA-seq library construction?
What is the purpose of using random hexamers instead of oligo(dT) primers for first strand synthesis in RNA-seq library construction?
What is the purpose of using random hexamers instead of oligo(dT) primers for first strand synthesis in RNA-seq library construction?
Which molecule is often removed from the total RNA pool before proceeding with library construction in RNA-seq?
Which molecule is often removed from the total RNA pool before proceeding with library construction in RNA-seq?
What is the purpose of selecting specifically for mRNAs using oligo(dT) beads in RNA-seq library construction?
What is the purpose of selecting specifically for mRNAs using oligo(dT) beads in RNA-seq library construction?
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