Podcast
Questions and Answers
Who coined the term 'plasmid'?
Who coined the term 'plasmid'?
- James Watson
- Francis Crick
- Joshua Lederberg (correct)
- Rosalind Franklin
What is the main function of plasmids in bacteria?
What is the main function of plasmids in bacteria?
- To provide antibiotic resistance
- To exchange genes with other bacteria
- To carry genes that benefit the survival of the organism (correct)
- To replicate independently of chromosomal DNA
What is the normal number of copies of plasmid found in a single cell called?
What is the normal number of copies of plasmid found in a single cell called?
- Cellular replication
- DNA number
- Plasmid count
- Copy number (correct)
What is the purpose of phenol-chloroform extraction in DNA purification?
What is the purpose of phenol-chloroform extraction in DNA purification?
Which of the following is a key application of PCR in forensic science?
Which of the following is a key application of PCR in forensic science?
What is the purpose of annealing in the PCR process?
What is the purpose of annealing in the PCR process?
What is the typical range for the GC content of PCR primers?
What is the typical range for the GC content of PCR primers?
What is the purpose of real-time PCR?
What is the purpose of real-time PCR?
Which detection method in real-time PCR involves the use of a fluorescent reporter molecule and a quencher molecule?
Which detection method in real-time PCR involves the use of a fluorescent reporter molecule and a quencher molecule?
What is the purpose of melting curve analysis in real-time PCR?
What is the purpose of melting curve analysis in real-time PCR?
Which method involves the synthesis of single-stranded DNA using single-stranded RNA as a template?
Which method involves the synthesis of single-stranded DNA using single-stranded RNA as a template?
Which type of virus uses RNA as its genetic material and replicates through reverse transcription?
Which type of virus uses RNA as its genetic material and replicates through reverse transcription?
Which enzyme is added to the pyrosequencing reaction mixture to catalyze the reaction of PPi with adenosine 5’ phosphosulfate (APS)?
Which enzyme is added to the pyrosequencing reaction mixture to catalyze the reaction of PPi with adenosine 5’ phosphosulfate (APS)?
What is the limitation of pyrosequencing when it comes to detecting homopolymeric regions?
What is the limitation of pyrosequencing when it comes to detecting homopolymeric regions?
What triggers the release of visible light in pyrosequencing?
What triggers the release of visible light in pyrosequencing?
Which sequencing technology utilizes reversible terminators?
Which sequencing technology utilizes reversible terminators?
Which of the following is a disadvantage of using qPCR for COVID-19 testing?
Which of the following is a disadvantage of using qPCR for COVID-19 testing?
What is the purpose of a Ct value in real-time PCR?
What is the purpose of a Ct value in real-time PCR?
What is the purpose of a housekeeping gene in relative quantification of gene expression?
What is the purpose of a housekeeping gene in relative quantification of gene expression?
How is the fold change in gene expression calculated in qRT-PCR?
How is the fold change in gene expression calculated in qRT-PCR?
Which of the following is NOT a method for rupturing cellular walls to isolate DNA and RNA from living cells?
Which of the following is NOT a method for rupturing cellular walls to isolate DNA and RNA from living cells?
What is the purpose of centrifugation in the separation of DNA by cesium chloride?
What is the purpose of centrifugation in the separation of DNA by cesium chloride?
Which macromolecule has the highest density and sinks to the bottom of the tube during centrifugation in the separation of DNA by cesium chloride?
Which macromolecule has the highest density and sinks to the bottom of the tube during centrifugation in the separation of DNA by cesium chloride?
What is the main purpose of extracting nucleic acids from living cells?
What is the main purpose of extracting nucleic acids from living cells?
Which of the following is a step involved in RNA-seq library construction?
Which of the following is a step involved in RNA-seq library construction?
What is the purpose of depleting rRNAs from the total RNA pool in RNA-seq?
What is the purpose of depleting rRNAs from the total RNA pool in RNA-seq?
Which primer can be used to specifically select for mRNAs in RNA-seq?
Which primer can be used to specifically select for mRNAs in RNA-seq?
What is the advantage of using random hexamers for first strand cDNA synthesis in RNA-seq?
What is the advantage of using random hexamers for first strand cDNA synthesis in RNA-seq?
What is the role of Ribonuclease H (RNase H) in second strand synthesis in RNA-seq?
What is the role of Ribonuclease H (RNase H) in second strand synthesis in RNA-seq?
What is the purpose of adding an 'A' base to the 3' ends of dsDNAs in RNA-seq library construction?
What is the purpose of adding an 'A' base to the 3' ends of dsDNAs in RNA-seq library construction?
Who discovered bacterial conjugation and won the Nobel Prize for it?
Who discovered bacterial conjugation and won the Nobel Prize for it?
What is the main function of plasmids in bacteria?
What is the main function of plasmids in bacteria?
What is the normal number of copies of plasmid found in a single cell called?
What is the normal number of copies of plasmid found in a single cell called?
What is the purpose of phenol-chloroform extraction in DNA purification?
What is the purpose of phenol-chloroform extraction in DNA purification?
Which enzyme is responsible for catalyzing the reaction of pyrophosphate (PPi) with adenosine 5’ phosphosulfate (APS) in pyrosequencing?
Which enzyme is responsible for catalyzing the reaction of pyrophosphate (PPi) with adenosine 5’ phosphosulfate (APS) in pyrosequencing?
What is the limitation of pyrosequencing when it comes to detecting homopolymeric regions?
What is the limitation of pyrosequencing when it comes to detecting homopolymeric regions?
Which sequencing technology utilizes reversible terminators?
Which sequencing technology utilizes reversible terminators?
What triggers the release of visible light in pyrosequencing?
What triggers the release of visible light in pyrosequencing?
Which detection method in real-time PCR involves the use of two sequence-specific oligonucleotide probes labeled with different dyes?
Which detection method in real-time PCR involves the use of two sequence-specific oligonucleotide probes labeled with different dyes?
Which of the following is a key application of PCR in forensic science?
Which of the following is a key application of PCR in forensic science?
What is the purpose of melting curve analysis in real-time PCR?
What is the purpose of melting curve analysis in real-time PCR?
What is the purpose of annealing in the PCR process?
What is the purpose of annealing in the PCR process?
What is reverse transcription?
What is reverse transcription?
What triggers the release of visible light in pyrosequencing?
What triggers the release of visible light in pyrosequencing?
What is the genetic material used by retroviruses?
What is the genetic material used by retroviruses?
What is the purpose of real-time PCR?
What is the purpose of real-time PCR?
Which of the following is a disadvantage of using Loop-Mediated Isothermal Amplification (LAMP) for COVID-19 testing?
Which of the following is a disadvantage of using Loop-Mediated Isothermal Amplification (LAMP) for COVID-19 testing?
What is the purpose of a cleavable hairpin beacon (CHB) in colorimetric detection of LAMP products?
What is the purpose of a cleavable hairpin beacon (CHB) in colorimetric detection of LAMP products?
What is the purpose of a standard curve in absolute quantification of gene expression?
What is the purpose of a standard curve in absolute quantification of gene expression?
What is the purpose of normalizing Ct values to a housekeeping gene in relative quantification of gene expression?
What is the purpose of normalizing Ct values to a housekeeping gene in relative quantification of gene expression?
Which of the following is NOT a method for rupturing cellular walls to isolate DNA and RNA from living cells?
Which of the following is NOT a method for rupturing cellular walls to isolate DNA and RNA from living cells?
What is the purpose of adding an 'A' base to the 3' ends of dsDNAs in RNA-seq library construction?
What is the purpose of adding an 'A' base to the 3' ends of dsDNAs in RNA-seq library construction?
What is the main function of plasmids in bacteria?
What is the main function of plasmids in bacteria?
What is the purpose of melting curve analysis in real-time PCR?
What is the purpose of melting curve analysis in real-time PCR?
Which step is involved in RNA-seq library construction?
Which step is involved in RNA-seq library construction?
What is the purpose of adding an 'A' base to the 3' ends of dsDNAs in RNA-seq library construction?
What is the purpose of adding an 'A' base to the 3' ends of dsDNAs in RNA-seq library construction?
What is the role of Ribonuclease H (RNase H) in second strand synthesis in RNA-seq?
What is the role of Ribonuclease H (RNase H) in second strand synthesis in RNA-seq?
Which method involves the synthesis of single-stranded DNA using single-stranded RNA as a template?
Which method involves the synthesis of single-stranded DNA using single-stranded RNA as a template?
What is the purpose of depleting rRNAs from the total RNA pool in RNA-seq?
What is the purpose of depleting rRNAs from the total RNA pool in RNA-seq?
What is the typical range for the GC content of PCR primers?
What is the typical range for the GC content of PCR primers?
Which of the following methods can be used to isolate DNA and RNA from living cells?
Which of the following methods can be used to isolate DNA and RNA from living cells?
How are DNA molecules separated from RNA and protein in the process of cesium chloride centrifugation?
How are DNA molecules separated from RNA and protein in the process of cesium chloride centrifugation?
What is the purpose of the polymerase chain reaction (PCR)?
What is the purpose of the polymerase chain reaction (PCR)?
What is the main function of plasmids in bacteria?
What is the main function of plasmids in bacteria?
Which enzyme is responsible for catalyzing the reaction of pyrophosphate (PPi) with adenosine 5’ phosphosulfate (APS) in pyrosequencing?
Which enzyme is responsible for catalyzing the reaction of pyrophosphate (PPi) with adenosine 5’ phosphosulfate (APS) in pyrosequencing?
What is the purpose of adding dATP-αS instead of natural dATP in pyrosequencing?
What is the purpose of adding dATP-αS instead of natural dATP in pyrosequencing?
What is the limitation of pyrosequencing when it comes to detecting homopolymeric regions?
What is the limitation of pyrosequencing when it comes to detecting homopolymeric regions?
What triggers the release of visible light in pyrosequencing?
What triggers the release of visible light in pyrosequencing?
Which detection method in real-time PCR involves the use of a fluorescent reporter molecule and a quencher molecule?
Which detection method in real-time PCR involves the use of a fluorescent reporter molecule and a quencher molecule?
What is the purpose of melting curve analysis in real-time PCR?
What is the purpose of melting curve analysis in real-time PCR?
Which type of virus uses RNA as its genetic material and replicates through reverse transcription?
Which type of virus uses RNA as its genetic material and replicates through reverse transcription?
What is the purpose of reverse transcription in molecular biology?
What is the purpose of reverse transcription in molecular biology?
Which of the following is a key application of qPCR in gene expression analysis?
Which of the following is a key application of qPCR in gene expression analysis?
What is the purpose of a standard curve in absolute quantification of gene expression using qPCR?
What is the purpose of a standard curve in absolute quantification of gene expression using qPCR?
What is the advantage of using relative quantification in gene expression analysis using qPCR?
What is the advantage of using relative quantification in gene expression analysis using qPCR?
What is the formula to calculate the fold change in gene expression using qPCR?
What is the formula to calculate the fold change in gene expression using qPCR?
Which of the following is NOT a characteristic of plasmids?
Which of the following is NOT a characteristic of plasmids?
Which of the following is TRUE about the copy number of plasmids?
Which of the following is TRUE about the copy number of plasmids?
Which of the following is TRUE about CsCl density gradient centrifugation?
Which of the following is TRUE about CsCl density gradient centrifugation?
What is the purpose of minipreparation of plasmid DNA?
What is the purpose of minipreparation of plasmid DNA?
Which step is involved in RNA-seq library construction?
Which step is involved in RNA-seq library construction?
What is the purpose of adding an 'A' base to the 3' ends of dsDNAs in RNA-seq library construction?
What is the purpose of adding an 'A' base to the 3' ends of dsDNAs in RNA-seq library construction?
What is the role of Ribonuclease H (RNase H) in second strand synthesis in RNA-seq?
What is the role of Ribonuclease H (RNase H) in second strand synthesis in RNA-seq?
What is the purpose of depleting rRNAs from the total RNA pool in RNA-seq?
What is the purpose of depleting rRNAs from the total RNA pool in RNA-seq?
What is the purpose of first strand cDNA synthesis with oligo(dT) in RNA-seq?
What is the purpose of first strand cDNA synthesis with oligo(dT) in RNA-seq?
What is the purpose of random hexamers in first strand synthesis in RNA-seq?
What is the purpose of random hexamers in first strand synthesis in RNA-seq?
What is the purpose of real-time PCR?
What is the purpose of real-time PCR?
What is the typical range for the length of PCR primers?
What is the typical range for the length of PCR primers?
What is the purpose of annealing in the PCR process?
What is the purpose of annealing in the PCR process?
What is the purpose of denaturation in the PCR process?
What is the purpose of denaturation in the PCR process?
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