Podcast
Questions and Answers
Virus replication is typically characterized by a one-step growth curve. Latent period consists of eclipse + ______
Virus replication is typically characterized by a one-step growth curve. Latent period consists of eclipse + ______
maturation
Bacterial viruses are easiest to grow; model systems. Animal viruses (and some plant viruses) can be cultivated in tissue or ______ cultures
Bacterial viruses are easiest to grow; model systems. Animal viruses (and some plant viruses) can be cultivated in tissue or ______ cultures
cell
Titer is the number of infectious units per volume of fluid [plaque-forming units (PFU) per ______]
Titer is the number of infectious units per volume of fluid [plaque-forming units (PFU) per ______]
millilitre
Plaque assay is analogous to the bacterial colony; one way to measure virus infectivity – Plaques are clear zones that develop on lawns of host cells. Each plaque results from infection by a single virus ______
Plaque assay is analogous to the bacterial colony; one way to measure virus infectivity – Plaques are clear zones that develop on lawns of host cells. Each plaque results from infection by a single virus ______
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Viral hosts can be classified based on the hosts they infect: Bacterial viruses, __________viruses, Animal viruse and Plant viruses
Viral hosts can be classified based on the hosts they infect: Bacterial viruses, __________viruses, Animal viruse and Plant viruses
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The protein shell that surrounds the genome of a virus particle is called ______
The protein shell that surrounds the genome of a virus particle is called ______
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Enveloped viruses have a membrane surrounding the nucleocapsid, which is a ______ bilayer with embedded proteins
Enveloped viruses have a membrane surrounding the nucleocapsid, which is a ______ bilayer with embedded proteins
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The complete complex of nucleic acid and protein packaged in the virion is known as ______
The complete complex of nucleic acid and protein packaged in the virion is known as ______
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Most viruses are smaller than ______ cells, ranging from 0.02 to 0.3 µm
Most viruses are smaller than ______ cells, ranging from 0.02 to 0.3 µm
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Phases of viral replication include: Attachment, Entry, Synthesis, Assembly, and Release of mature ______ from the host cell
Phases of viral replication include: Attachment, Entry, Synthesis, Assembly, and Release of mature ______ from the host cell
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The cell–phage mixture is poured onto a solidified nutrient agar plate. Mixture containing molten top agar, bacterial cells, and diluted phage suspension Nutrient agar plate. The mixture is left to solidify. Sandwich of top agar and nutrient agar Phage ______
The cell–phage mixture is poured onto a solidified nutrient agar plate. Mixture containing molten top agar, bacterial cells, and diluted phage suspension Nutrient agar plate. The mixture is left to solidify. Sandwich of top agar and nutrient agar Phage ______
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Incubation allows for bacterial growth and phage replication. Lawn of host cells ______
Incubation allows for bacterial growth and phage replication. Lawn of host cells ______
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Attachment of virion to host cell is highly specific – Requires complementary receptors on the surface of a susceptible host and its infecting virus. Receptors on host cell carry out normal functions for cell (e.g., uptake proteins, cell-to-cell interaction). Receptors include proteins, carbohydrates, glycoproteins, lipids, lipoproteins, or complexes BACTERIOPHAGE RECEPTORS. Chi Flagellum Pilus M13 T1 MS2 Outer membrane Peptidoglycan ______
Attachment of virion to host cell is highly specific – Requires complementary receptors on the surface of a susceptible host and its infecting virus. Receptors on host cell carry out normal functions for cell (e.g., uptake proteins, cell-to-cell interaction). Receptors include proteins, carbohydrates, glycoproteins, lipids, lipoproteins, or complexes BACTERIOPHAGE RECEPTORS. Chi Flagellum Pilus M13 T1 MS2 Outer membrane Peptidoglycan ______
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The attachment of a virus to its host cell results in changes to both virus and cell surface that facilitate penetration. Permissive cell: host cell that allows the complete replication cycle of a virus to occur ______
The attachment of a virus to its host cell results in changes to both virus and cell surface that facilitate penetration. Permissive cell: host cell that allows the complete replication cycle of a virus to occur ______
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Plating efficiency is used in quantitative virology. The number of plaque-forming units is almost always lower than direct counts by electron microscopy. Inactive virions Conditions not appropriate for infectivity ______
Plating efficiency is used in quantitative virology. The number of plaque-forming units is almost always lower than direct counts by electron microscopy. Inactive virions Conditions not appropriate for infectivity ______
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Bacteriophage T4: virus of ______
Bacteriophage T4: virus of ______
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