19 Questions
What is the purpose of a selectable marker in gene transfer using a plasmid?
To eliminate bacteria that have not been genetically modified
How does gene therapy work in treating genetic diseases?
By introducing a healthy gene into the patient's cells
What is the main function of a recombinant plasmid?
To act as a carrier of the gene of interest to host cells
In gene therapy, what is a common method for introducing genes into patients' cells?
Using adeno-associated viruses
Why are plasmids used in creating disease models?
To generate rat genetic models
What is an essential step for a gene of interest to be transferred into host cells?
It must be inserted into a cloning vector like a plasmid
What happens when a virulence plasmid is inside a bacterium?
It turns the bacterium into a disease agent
How are disease models created using plasmids different from gene therapy?
Disease models help understand diseases by creating models while gene therapy introduces healthy genes to cure diseases.
Why are antibiotic resistance genes commonly used as selectable markers in cloning vectors?
To help identify which cells have taken up the plasmid of interest.
What is the purpose of a recombinant plasmid?
To carry the gene of interest into host cells
Why are plasmids used in gene therapy?
To insert a healthy gene into human cells
Which method is commonly used to ensure that only genetically modified bacteria survive in cloning vectors?
Including selectable markers like antibiotic resistance genes
What is the main function of a selectable marker in cloning vectors?
To select cells with the plasmid of interest
In which organism can a virulence plasmid turn into a disease agent?
Humans
How do plasmids contribute to insulin production?
By carrying the insulin gene into bacteria for multiplication
What allows for the selection of genetically modified bacteria in cloning vectors?
Including antibiotic resistance genes as selectable markers
What is the primary role of a virulence plasmid inside a bacterium?
Turning into a pathogen
How are genes transferred into host cells using plasmids?
By inserting them into a cloning vector
Why are antibiotic resistance genes commonly used as selectable markers?
To allow selection of cells with the plasmid of interest
Learn about plasmids, small circular DNA molecules separate from a host cell's chromosome. Discover how they replicate independently, their symbiotic or parasitic interactions with host cells, and their role as replicons.
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