Understanding Plasmids in Biology

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Questions and Answers

What is the purpose of a selectable marker in gene transfer using a plasmid?

  • To kill the host cells
  • To eliminate bacteria that have not been genetically modified (correct)
  • To replicate the plasmid in the host cells
  • To increase the chance of gene expression in the host cells

How does gene therapy work in treating genetic diseases?

  • By reducing the replication of healthy genes in the patient's cells
  • By removing all genes from the patient's cells
  • By introducing a healthy gene into the patient's cells (correct)
  • By increasing the number of defective genes in the patient's cells

What is the main function of a recombinant plasmid?

  • To produce insulin in bacteria
  • To act as a carrier of the gene of interest to host cells (correct)
  • To remove defective genes from host cells
  • To cure diseases directly

In gene therapy, what is a common method for introducing genes into patients' cells?

<p>Using adeno-associated viruses (D)</p> Signup and view all the answers

Why are plasmids used in creating disease models?

<p>To generate rat genetic models (C)</p> Signup and view all the answers

What is an essential step for a gene of interest to be transferred into host cells?

<p>It must be inserted into a cloning vector like a plasmid (D)</p> Signup and view all the answers

What happens when a virulence plasmid is inside a bacterium?

<p>It turns the bacterium into a disease agent (A)</p> Signup and view all the answers

How are disease models created using plasmids different from gene therapy?

<p>Disease models help understand diseases by creating models while gene therapy introduces healthy genes to cure diseases. (A)</p> Signup and view all the answers

Why are antibiotic resistance genes commonly used as selectable markers in cloning vectors?

<p>To help identify which cells have taken up the plasmid of interest. (D)</p> Signup and view all the answers

What is the purpose of a recombinant plasmid?

<p>To carry the gene of interest into host cells (D)</p> Signup and view all the answers

Why are plasmids used in gene therapy?

<p>To insert a healthy gene into human cells (B)</p> Signup and view all the answers

Which method is commonly used to ensure that only genetically modified bacteria survive in cloning vectors?

<p>Including selectable markers like antibiotic resistance genes (C)</p> Signup and view all the answers

What is the main function of a selectable marker in cloning vectors?

<p>To select cells with the plasmid of interest (D)</p> Signup and view all the answers

In which organism can a virulence plasmid turn into a disease agent?

<p>Humans (D)</p> Signup and view all the answers

How do plasmids contribute to insulin production?

<p>By carrying the insulin gene into bacteria for multiplication (D)</p> Signup and view all the answers

What allows for the selection of genetically modified bacteria in cloning vectors?

<p>Including antibiotic resistance genes as selectable markers (B)</p> Signup and view all the answers

What is the primary role of a virulence plasmid inside a bacterium?

<p>Turning into a pathogen (C)</p> Signup and view all the answers

How are genes transferred into host cells using plasmids?

<p>By inserting them into a cloning vector (B)</p> Signup and view all the answers

Why are antibiotic resistance genes commonly used as selectable markers?

<p>To allow selection of cells with the plasmid of interest (D)</p> Signup and view all the answers

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