Understanding Kingdom Protista

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Questions and Answers

Given the endosymbiotic theory and the diversity observed within protists, which evolutionary scenario is least plausible regarding the origin of protistan organelles?

  • Secondary endosymbiosis, where a eukaryotic cell engulfed another eukaryotic cell containing plastids, resulted in diverse algal groups.
  • Serial endosymbiosis involving multiple engulfment events led to the complex plastids found in certain protist lineages.
  • Horizontal gene transfer events from prokaryotes to early eukaryotic cells directly generated novel organelle functions, bypassing endosymbiosis. (correct)
  • Mitochondria originated from a single endosymbiotic event involving an alpha-proteobacterium.

Considering the ecological roles of protists, which alteration to a marine environment would most severely disrupt global biogeochemical cycles?

  • Extinction of all silica-based diatoms. (correct)
  • Complete removal of all freshwater amoebas.
  • Selective extinction of all parasitic protists.
  • Elimination of all nitrogen-fixing bacteria.

In the context of protistan osmoregulation, what biophysical principle most critically underlies the function of contractile vacuoles, especially in freshwater species?

  • Reverse osmosis, expelling water molecules while retaining essential ions, thus preventing cell lysis.
  • Osmosis driven by a hypertonic intracellular environment relative to the hypotonic surroundings. (correct)
  • Active transport of solutes against their concentration gradients, maintaining cellular turgor.
  • Facilitated diffusion of water molecules through aquaporins, equilibrating osmotic pressure.

Given the diversity of reproductive strategies in protists, which evolutionary pressure would most strongly favor the prevalence of sexual reproduction over asexual reproduction in a protistan population?

<p>Rapidly changing environmental conditions with high selective pressures. (B)</p> Signup and view all the answers

Considering the pathogenesis of Naegleria fowleri, which mechanism would most effectively prevent infection in recreational water sources?

<p>Maintaining consistently low water temperatures below the organism's tolerable range. (C)</p> Signup and view all the answers

Suppose a novel compound is discovered that selectively disrupts the function of the apical complex in apicomplexan parasites. What is the most likely consequence of this disruption?

<p>Reduced capacity for host cell invasion and parasite establishment. (B)</p> Signup and view all the answers

In the context of diatom physiology, propose a mechanism by which ocean acidification could disrupt diatom populations, considering their unique cell wall structure.

<p>Increased dissolution of the silica frustules, compromising structural integrity and protection. (A)</p> Signup and view all the answers

Assuming that the micronucleus of a ciliate sustains irreparable damage preventing genetic exchange, what long-term evolutionary consequence is most likely for the ciliate lineage?

<p>Reduced adaptive potential and increased susceptibility to environmental stressors. (D)</p> Signup and view all the answers

Consider a scenario where a novel, highly specific algicide is introduced into a freshwater ecosystem, targeting only euglenoids. Which ecological consequence is least likely to occur?

<p>An immediate increase in dissolved oxygen levels due to the elimination of euglenoid respiration. (C)</p> Signup and view all the answers

Given the complex life cycle of Plasmodium, what intervention strategy would most effectively disrupt transmission in a region with widespread insecticide resistance in Anopheles mosquitoes?

<p>Deployment of genetically modified mosquitoes incapable of supporting <em>Plasmodium</em> development. (A)</p> Signup and view all the answers

In the context of slime mold behavior, what cellular mechanism primarily drives the coordinated movement and aggregation of individual amoeboid cells to form a migrating pseudoplasmodium?

<p>Chemotaxis mediated by cyclic AMP (cAMP) gradients, guiding cell movement towards aggregation centers. (B)</p> Signup and view all the answers

Considering the ecological impact of dinoflagellate blooms (red tides), which environmental change would most likely exacerbate the frequency and intensity of these events in coastal waters?

<p>Increased coastal upwelling, bringing nutrient-rich deep waters to the surface. (A)</p> Signup and view all the answers

If a protist under microscopic investigation exhibits both a gullet and contractile vacuole, alongside cilia, which classification is most accurate?

<p>Ciliophora. (B)</p> Signup and view all the answers

Given the heterotrophic behavior of amoebas, what external stimuli would activate the formation of pseudopodia?

<p>The detection of a higher concentration gradient of nutrient molecules. (D)</p> Signup and view all the answers

What evolutionary pressure would most likely result in the loss of chloroplasts in previously photosynthetic protists?

<p>An adaptation to life in permanently dark environments, such as deep-sea hydrothermal vents. (D)</p> Signup and view all the answers

In a scenario where global temperatures rise significantly, leading to widespread coral bleaching, what secondary effect is most likely to occur due to the loss of symbiotic dinoflagellates?

<p>Elevated susceptibility to disease and reduced resilience to environmental stressors. (B)</p> Signup and view all the answers

Of the methods listed, which is the most likely explanation for the rise of multicellular protists?

<p>The co-option of existing unicellular signaling pathways for intercellular communication. (B)</p> Signup and view all the answers

If an unknown compound is added to a protist colony and the protists all demonstrate cell lysis, which cellular component was likely affected?

<p>Plasma Membrane. (B)</p> Signup and view all the answers

When observing a sample of aquatic protists, one is seen to be significantly larger with intricate geometry and what appears to be a glass-like shell. Which of the following is the most likely identification?

<p>Diatom. (A)</p> Signup and view all the answers

If one was to compare a species of simple plant and a species of plant-like protist, what would be the most notable disparity?

<p>The lack of complex tissues. (D)</p> Signup and view all the answers

Which evolutionary event is most likely needed for protists to transition to extremely differentiated multicellular life akin to plants/animals?

<p>The evolution of differentiation and compartmentalization. (B)</p> Signup and view all the answers

When considering the tree of life, which of the following groupings is most accurate?

<p>Protists as non-monophyletic. (B)</p> Signup and view all the answers

What evidence most strongly supports the hypothesis that protists are critical in carbon cycling?

<p>Their substantial contribution to global photosynthesis and decomposition processes. (C)</p> Signup and view all the answers

When considering the kingdom Protista, what criteria could be used to reclassify the kingdom into valid monophyletic taxa?

<p>The existence and role of the endosymbiosis of chloroplasts. (B)</p> Signup and view all the answers

If one were to investigate the effect of various toxins on protists, which physiological feature would be useful for classifying unknown toxicants?

<p>Inhibition and effect on the photosynthetic activities. (B)</p> Signup and view all the answers

What is the most accurate conclusion in a controlled environment where specific protists were observed to flourish?

<p>The controlled conditions were similar/identical to their natural habitat. (D)</p> Signup and view all the answers

In a hypothetical engineering of a protist, which artificial addition would optimize energy intake and survivability in aquatic ecosystem?

<p>An artificial organelle that can facilitate unique nutrient usage. (A)</p> Signup and view all the answers

If a patient is infected with a protist attributed to consuming unfiltered water, what is the most likely treatment pathway?

<p>Directly targeting cellular targets of the protist by using specific medication. (B)</p> Signup and view all the answers

What selective advantage is most likely to have driven the evolution of mixotrophy in protists inhabiting fluctuating environments?

<p>The capacity to exploit both autotrophic and heterotrophic modes of nutrition. (A)</p> Signup and view all the answers

Of the options listed, which could be used to classify different protists based on their mode of locomotion?

<p>By the presence of flagella, cilia, or pseudopodia. (A)</p> Signup and view all the answers

In a highly competitive environment, what mechanism would be most beneficial for a protist to maintain their position?

<p>Ability to reproduce quicker via asexual production. (D)</p> Signup and view all the answers

In a research experiment, it was found that protists are responsible for a previously unknown symbiotic relationship. How would this discovery affect ecological models?

<p>By requiring ecosystem models to have a greater complexity. (D)</p> Signup and view all the answers

How does the study of protists impact the development of novel drug therapies?

<p>The discovery of novel mechanisms might lead to bio-inspired therapeutics. (D)</p> Signup and view all the answers

Which is most likely, what impact would occur if protists were extinct?

<p>Significant alteration of ecological food chain. (B)</p> Signup and view all the answers

How would climate change most drastically impact protist distribution? (Assuming a gradual uniform climate change)

<p>Geographic relocation may result in competition. (D)</p> Signup and view all the answers

In what way would our understanding of evolutionary history shift if protists were excluded from models?

<p>Existing models would be significantly lacking. (A)</p> Signup and view all the answers

If a protist species is discovered and known to increase carbon sequestration in aquatic environments, which of the following is an appropriate usage?

<p>Immediate study and replication in research. (B)</p> Signup and view all the answers

Which scenario is the most likely outcome of increased ocean stratification due to global warming on protist communities?

<p>Shifted protist-community structures, favoring species adapted to low nutrient conditions. (C)</p> Signup and view all the answers

How does understanding protistan biology inform strategies for managing and mitigating waterborne diseases in developing countries?

<p>By revealing novel drug targets and transmission pathways for parasitic protists. (A)</p> Signup and view all the answers

When considering synthetic biology and protists, what benefit can come from combining the two?

<p>Engineering protists to perform certain tasks. (D)</p> Signup and view all the answers

If global biodiversity is negatively affected outside of the protist kingdom, what mechanisms could be utilized that involves protists to stop destruction?

<p>Utilizing certain protists to target factors causing environmental disruptions. (C)</p> Signup and view all the answers

Flashcards

Kingdom Protista

Eukaryotic microbes, the first to have a true nucleus.

Protist Ecological Diversity

Vast across land and sea, with 65K-200K species. Includes amoebas, paramecium, and diatoms.

Protista Placement

Kingdom for organisms that don't fit into other kingdoms.

Eukaryotic

Organisms with cells that contain a nucleus.

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Protist cell number

Most protists are made of one cell. Some are simple multicellular.

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Protist Reproduction

Asexual reproduction: binary fission, multiple fission, or mitosis. also sexually (meiosis).

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Protist categories

Unicellular protists categorized as animal-like, plant-like and fungus-like.

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Animal-like protists

Usually called protozoa; heterotrophic and unicellular.

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Contractile Vacuole

Organelle that removes excess water in animal-like protists.

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Amoeba Habitat and Mobility

Amoeba habitat includes moist soil, water, and even human-made pipelines. They have pseudopods for mobility.

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Brain-eating Amoeba

Naegleria fowleri; It feeds on brain tissue and is infected through the nose.

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Cilia

Structures used for locomotion and particle manipulation by paramecium.

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Paramecium Unique Cell Organelles

Oral groove and gullet, two nuclei (macro and micro).

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Macronucleus Function

The macronucleus provides the nuclear RNA for vegetative growth.

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Micronuclei

Exchange of haploid micronuclei is involved in sexual reproduction.

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Flagellates Habitat

Found in aquatic environments, part of mutualistic or parasitic relationships.

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Flagellates Organelles

Elongated body and flagella, for mobility.

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Sporozoans Lifestyle

A parasitic lifestyle often within 2 different host species.

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Plant-like Protists

They commonly called algae; have Chloroplasts for photosynthesis.

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Diatoms

Produce much oxygen via photosynthesis; cell walls of silica.

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Diatoms Structure

Cell wall coated in silica in shoebox style.

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Diatoms Reproduction

Mostly asexual mitosis, but meiosis occurs during unfavorable conditions.

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Dinoflagellates

Phytoplankton living in water environments, possess flagella that cause them to spin.

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Dinoflagellates Blooms

Algae blooms, causing death/toxicity to marine life. Mutualism with coral gives them color.

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Euglenoids

The stigma (photoreceptor) allows flagellum to position the toward the light.

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Fungus-like Protists

Resemble true fungi but differ at the cellular level (cell wall). Reproduce with spores.

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Plasmodial Slime Molds

They start out as cells and join to form a plasmodium super-cell to digest matter.

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Plasmodium Reproduction

Become sporangia when food runs out. Dispersal by spores is fungi-like.

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Study Notes

Kingdom Protista

  • Protists are eukaryotic microbes and were the first to develop a true nucleus.
  • This kingdom possesses vast ecological diversity of approximately 65,000 to 200,000 species.
  • Examples of protists include amoebas, paramecium, and diatoms.
  • Any organism that does not fit into other kingdoms is placed into the Protista kingdom.
  • Some protist species are large enough to be seen with the naked eye.

Characteristics of Protists

  • Eukaryotic organisms
  • Most are unicellular, but some are simple multicellular organisms.
  • Some reproduce asexually via binary fission, multiple fission, or mitosis.
  • Some reproduce sexually via meiosis.
  • Most live in aquatic environments, damp areas like moist soil, or within other organisms.
  • Protists include fungi, plants, and animals.

Protist Classification

  • Protists are their own kingdom.
  • Protists can be divided into three groups: plant-like protists, fungi-like protists, and animal-like protists.

Unicellular Protist Groups

  • Animal-like protists are also known as protozoans and include amoebas, ciliates, and flagellates.
  • Plant-like protists include euglenoids, diatoms, and dinoflagellates.
  • Fungus-like protists include slime molds and water molds.
  • Animal-like protists consume other organisms, and some are parasitic.
  • Plant-like protists make their own food via photosynthesis and may consume organisms when light is unavailable. Some are symbionts.
  • Fungus-like protists absorb nutrients from other living or dead organisms. Some consume organisms and some water molds are parasites.

Animal-like Protists

  • Animal-like protists are often called protozoa.
  • Heterotrophic organisms.
  • Unicellular organisms.
  • Often have contractile vacuoles that remove excess water.
  • Live in aquatic environments, and may be predators or decomposers, or may form symbiotic relationships within other organisms such as parasitism, mutualism, or commensalism.
  • Classified by their movement.

Cercozoans: Amoeba

  • Cercozoans are found in moist soil and water, including human-made pipelines, and within organisms.
  • Heterotrophic organisms.
  • Move using pseudopods, also known as "fake feet," which are extensions of cytoplasm that are used to ingest food.
  • Unique cell organelles include a cytoplasm, plasma membrane, and nucleus; typically lack cilia and flagella.

Brain-eating Amoeba

  • Naegleria fowleri
  • Found in warm lakes, ponds, untreated swimming pools, hot springs, aquariums, and moist soil.
  • Consume bacteria but can feed on brain tissue when infecting humans.
  • Infection typically occurs through the nose during water sports such as diving or water skiing.
  • Symptoms include headache, fever, stiff neck, loss of appetite, vomiting, seizures, and coma.

Ciliates: Paramecium

  • Ciliates live in bodies of water, mostly stagnant water, and within other organisms.
  • Heterotrophic organisms.
  • Use cilia for locomotion and particle manipulation.
  • Unique organelles include oral groove and gullet; cilia move nutrients into the gullet. Have two nuclei, macro and micro.
  • The macronucleus provides the nuclear RNA for vegetative growth
  • Mating cells exchange haploid micronuclei and a new macronucleus develops from a new diploid micronucleus, therefore micronuclei are involved in sexual reproduction.

Flagellates

  • Inhabit aquatic environments, snow, and multicellular organisms, existing in mutualistic or parasitic relationships.
  • Heterotrophic organisms.
  • Use one or more flagella for mobility.
  • Feature an elongated body and flagella.

Sporozoans

  • Mostly parasitic
  • Heterotrophic
  • Reproduce via binary fission and alternate between sexual and asexual reproduction.
  • Move without flagella, cilia, or pseudopods.
  • Parasitic lifecycle that occurs within two different host species, and have a spore-like stage.

Plant-like Protists

  • Commonly called algae.
  • Chloroplasts allow for photosynthesis, primarily autotrophic but some are mixotrophic.
  • Accessory pigments give a variety of colors like red and green.
  • Reproduce asexually, most common, and sexually.
  • Live in moist environments such as ponds.

Diatoms

  • Diatoms produce a lot of oxygen through photosynthesis from 20-50% generated.
  • Made of silica
  • Dead diatoms shells make up a significant portion of the ocean floor which is up to 800 meters in some places.
  • Cell walls coated in silica in shoebox style
  • Important food source of aquatic organisms.

Diatoms: Phylum Chrysophyta

  • The most abundant type of phytoplankton lives in aquatic environments and are unicellular.
  • Diatoms are autotrophic.
  • They reproduce through mitosis in asexual reproduction, and meiosis for sexual reproduction.
  • Are free-floating.
  • Their cell walls are coated in silica in a shoebox style.
  • Reproduce mostly by asexual mitosis but under unfavorable conditions will reproduce by meiosis.

Dinoflagellates

  • Phytoplankton that inhabits aquatic environments
  • Autotrophic organisms.
  • Flagella allows them to spin.
  • Have two flagella positioned 90° or perpendicular to one another.
  • Pigments give distinctive colors.
  • Rapid reproduction in time of optimal conditions and abundant nutrients gives rise to algae blooms, causing either death or increased toxicity to marine life.
  • Mutualism while living within coral gives the coral their color; when reefs warm, coral expels dinoflagellates.

Euglenoids

  • Found in shallow water.
  • Autotrophic, but some can switch to heterotrophic when needed.
  • Move via flagellum.
  • Stigma (photoreceptor) allows flagellum to position the protist towards the light, or away from light.

Algae

  • Most are aquatic but some are terrestrial and can be found on moist soil, trees, and rocks.
  • Most are unicellular however some are multicellular, except for some brown algae which are multicellular.
  • Autotrophic organisms.
  • Reproduction via Mitosis (asexual) or Meiosis (sexual).
  • Float, or are attached to objects within water or on land.
  • Differ from vascular plants by lacking true roots, stems, and leaves.
  • Algae include Phylum Chlorophyta (green algae), Phylum Phaeophyta (brown algae), Phylum Rhodophyta (red algae), Phylum Chrysophyta (golden algae).

Fungus-like Protists

  • Resemble fungi in appearance and lifestyle, but differ from true fungi at the cellular level, such as the cell wall.
  • Reproduce with spore
  • Heterotrophs that feed on bacteria and rotting material, such as water mold & slime mould
  • Dog Vomit Slime mold

Plasmodial Slime Molds

  • Start as individual amoeboid cell but will join to form a multi-nucleate mass having only one cellular membrane called a plasmodium.
  • This plasmodium moves over dead to decaying material looking for bacteria to engulf or digest.
  • When plasmodium runs out of food it will produce fruiting structures called Sporangia, which has diploid nuclei that goes under meiosis, producing spores for aerial dispersal.
  • Dispersal by spores makes it fungi-like and once landed will germinate into haploid cells two flagella.

Cellular Slime Molds

  • Rarely can be visible to the naked eye.
  • Have one nuclei in each at times.
  • Individuals may join to form a pseudoplasmodium.

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