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Understanding Fastq File Format

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Questions and Answers

What does the 'TLEN' in a BAM file represent?

Quality score of sequencing

Number of total reads

Template length of reference covered by paired-end reads (correct)

Distance between reads

What type of file is a BAM file?

Plain text

Executable file

Image file

Compressed binary alignment file (correct)

In the PDB file format, which section contains information about the protein and experiment?

Coordinates

Reference sequence

Header (correct)

Quality scores

What does the 'QUAL' column in a BAM file hold?

PHRED score values of reads Signup and view all the answers

What is the significance of the '*' symbol in the 'QUAL' column of a BAM file?

Missing quality score values Signup and view all the answers

What is the primary function of a BAM file?

Store nucleotide sequence alignments Signup and view all the answers

What tool is typically required to view the contents of a BAM file?

Samtools Signup and view all the answers

What does the 'PDB' file format stand for?

Protein Data Bank Signup and view all the answers

Which column in a BAM file represents the sequence of the read or segment?

SEQ Signup and view all the answers

What information does the 'Header' section of a PDB file contain?

Experimental details Signup and view all the answers