Understanding Fastq File Format

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Questions and Answers

What does the 'TLEN' in a BAM file represent?

  • Quality score of sequencing
  • Number of total reads
  • Template length of reference covered by paired-end reads (correct)
  • Distance between reads

What type of file is a BAM file?

  • Plain text
  • Executable file
  • Image file
  • Compressed binary alignment file (correct)

In the PDB file format, which section contains information about the protein and experiment?

  • Coordinates
  • Reference sequence
  • Header (correct)
  • Quality scores

What does the 'QUAL' column in a BAM file hold?

<p>PHRED score values of reads (A)</p> Signup and view all the answers

What is the significance of the '*' symbol in the 'QUAL' column of a BAM file?

<p>Missing quality score values (C)</p> Signup and view all the answers

What is the primary function of a BAM file?

<p>Store nucleotide sequence alignments (A)</p> Signup and view all the answers

What tool is typically required to view the contents of a BAM file?

<p>Samtools (A)</p> Signup and view all the answers

What does the 'PDB' file format stand for?

<p>Protein Data Bank (B)</p> Signup and view all the answers

Which column in a BAM file represents the sequence of the read or segment?

<p>SEQ (D)</p> Signup and view all the answers

What information does the 'Header' section of a PDB file contain?

<p>Experimental details (B)</p> Signup and view all the answers

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