Understanding Bacterial Monitoring in Water

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Questions and Answers

What is the purpose of monitoring for pathogens in water?

To use indicator species to suggest fecal contamination (correct)
To measure the succession of microorganisms during self-purification
To examine microbial populations using PCR detection
To directly monitor the presence of all pathogens

Which method is currently the most used approach for isolating and enumerating microbial cells?

Real Time PCR quantification
Fluorescent in situ hybridization (FISH)
Flow cytometry
Sample plating and counting (correct)

What is the Great Plate Count Anomaly related to?

Microbial fingerprinting
Immunological methods for examining microbial populations
Habitat and cultivable percentage of microbial cells (correct)
Activity measurements of microorganisms

What is a potential source of bacteria mentioned in the text?

Activated sludge (A) Signup and view all the answers

What does the presence of certain microorganisms in water suggest?

Fecal contamination may have occurred (B) Signup and view all the answers

What are the potential sources of bacterial contamination mentioned in the text?

The potential sources of bacterial contamination mentioned in the text include fecal waste, tap water, activated sludge, sediments, and soils. Signup and view all the answers

What are the reasons for monitoring bacteria in water?

The reasons for monitoring bacteria in water include the succession of microorganisms during the self-purification process, the association of pathogenic microorganisms with fecal waste and the potential to cause diseases, and the difficulty in directly monitoring various pathogens, leading to the use of indicator species to suggest fecal contamination. Signup and view all the answers

What are some methods mentioned in the text for examining microbial populations?

The methods mentioned for examining microbial populations include microscopy, activity measurements, culture methods such as the Great Plate Count Anomaly and MPN, immunological methods, molecular detection methods like PCR and Real Time PCR quantification, and whole cell analysis using techniques like Fluorescent in situ Hybridisation (FISH) and flow cytometry. Signup and view all the answers

What is the Great Plate Count Anomaly related to?

The Great Plate Count Anomaly is related to the discrepancy between the estimated number of bacteria based on direct microscopic counts and the number of viable bacteria obtained by cultivation on agar plates. Signup and view all the answers

What are the habitat types and their respective cultivable percentages mentioned in the text?

The habitat types mentioned in the text are seawater, freshwater, mesotrophic lake, tap water, activated sludge, sediments, and soils. The respective cultivable percentages range from 0.001-0.1% in seawater to 0.25-15% in activated sludge. Signup and view all the answers