Viral purification and concentration

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Questions and Answers

What is the primary role of ultracentrifuges in virology?

  • To increase the temperature of viral cultures
  • To enhance viral replication
  • To concentrate and purify viruses (correct)
  • To destroy viral particles

Why are ultracentrifuges particularly useful for studying viruses

  • They can sediment even the smallest viruses (correct)
  • They allow viruses to multiply rapidly
  • They prevent viral contamination
  • They reduce the infectivity of viruses

What is the main principle behind rate-zonal centrifugation?

  • Particles are separated based on their buoyant density
  • Particles move at different rates depending on their mass (correct)
  • Particles aggregate at the bottom of the centrifuge tube
  • Particles are destroyed through high-speed spinning

Which type of centrifugation separates particles solely based on their buoyant density?

<p>Isopycnic (equilibrium) centrifugation (B)</p> Signup and view all the answers

Another term for isopycnic centrifugation is

<p>Density gradient separation (B)</p> Signup and view all the answers

In rate-zonal centrifugation, what determines how fast a particle moves through the medium?

<p>Its mass (C)</p> Signup and view all the answers

Flashcards

What is the primary role of ultracentrifuges in virology?

To separate and concentrate viruses based on their size and density.

Why are ultracentrifuges particularly useful for studying viruses?

They can isolate even the smallest viruses by separating them based on their sedimentation rate in a density gradient.

What is the main principle behind rate-zonal centrifugation?

This method separates particles based on their size and sedimentation rate. Larger and denser particles move faster through the gradient.

Which type of centrifugation separates particles solely based on their buoyant density?

This type of centrifugation separates particles based on their density, with denser particles settling at the bottom of the gradient.

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Another term for isopycnic centrifugation is

This process separates particles based on their density, with denser particles settling at the bottom of the gradient.

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In rate-zonal centrifugation, what determines how fast a particle moves through the medium?

The mass of a particle determines how fast it moves through the medium in rate-zonal centrifugation. Larger and denser particles move faster.

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