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Ultra Microtome Operation and Components
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Ultra Microtome Operation and Components

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Questions and Answers

What is the primary use of an ultra microtome?

  • Sterilizing laboratory equipment
  • Cutting ultrathin sections for transmission electron microscopy (correct)
  • Magnifying small objects for better visibility
  • Analyzing the chemical composition of tissues
  • Which factor facilitates section cutting when using an ultra microtome?

  • Using a higher frequency blade
  • Increasing the temperature
  • Applying pressure to the sections
  • Lowering the temperature (correct)
  • A higher rake angle in an ultra microtome helps in:

  • Increasing the temperature
  • Smooth flow of ribbons (correct)
  • Slower cutting of soft tissues
  • Cutting hard tissues
  • Which of the following is NOT a part of the ultra microtome as described?

    <p>Magnification Control</p> Signup and view all the answers

    How does the consistency of the tissue affect sectioning in an ultra microtome?

    <p>Soft tissue is cut at a slower rate than hard tissue</p> Signup and view all the answers

    Study Notes

    Ultra Microtome

    • Used to cut ultrathin sections for transmission electron microscopy
    • Cuts sections between 40 and 100 nanometric thickness
    • Uses glass or diamond knife for cutting

    Factors Involved in Cutting

    • Lowering the temperature facilitates section cutting
    • Higher rake angle helps in smooth flow of ribbons
    • Lower rake angle is used for hard tissue
    • Soft tissue is cut at a slower rate than hard tissue

    Parts of Microtome

    • Wheel Lock (A)
    • Knife Stage (B)
    • Knife Angle Locking Lever (C)
    • Knife Locking Lever (D)

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    Description

    This quiz covers the basics of ultra microtome, including its use in transmission electron microscopy, factors involved in cutting, and its various parts. Topics include cutting techniques, knife angles, and tissue handling.

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