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Questions and Answers
What is the primary use of an ultra microtome?
What is the primary use of an ultra microtome?
- Sterilizing laboratory equipment
- Cutting ultrathin sections for transmission electron microscopy (correct)
- Magnifying small objects for better visibility
- Analyzing the chemical composition of tissues
Which factor facilitates section cutting when using an ultra microtome?
Which factor facilitates section cutting when using an ultra microtome?
- Using a higher frequency blade
- Increasing the temperature
- Applying pressure to the sections
- Lowering the temperature (correct)
A higher rake angle in an ultra microtome helps in:
A higher rake angle in an ultra microtome helps in:
- Increasing the temperature
- Smooth flow of ribbons (correct)
- Slower cutting of soft tissues
- Cutting hard tissues
Which of the following is NOT a part of the ultra microtome as described?
Which of the following is NOT a part of the ultra microtome as described?
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How does the consistency of the tissue affect sectioning in an ultra microtome?
How does the consistency of the tissue affect sectioning in an ultra microtome?
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Study Notes
Ultra Microtome
- Used to cut ultrathin sections for transmission electron microscopy
- Cuts sections between 40 and 100 nanometric thickness
- Uses glass or diamond knife for cutting
Factors Involved in Cutting
- Lowering the temperature facilitates section cutting
- Higher rake angle helps in smooth flow of ribbons
- Lower rake angle is used for hard tissue
- Soft tissue is cut at a slower rate than hard tissue
Parts of Microtome
- Wheel Lock (A)
- Knife Stage (B)
- Knife Angle Locking Lever (C)
- Knife Locking Lever (D)
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