5 Questions
What is the primary use of an ultra microtome?
Cutting ultrathin sections for transmission electron microscopy
Which factor facilitates section cutting when using an ultra microtome?
Lowering the temperature
A higher rake angle in an ultra microtome helps in:
Smooth flow of ribbons
Which of the following is NOT a part of the ultra microtome as described?
Magnification Control
How does the consistency of the tissue affect sectioning in an ultra microtome?
Soft tissue is cut at a slower rate than hard tissue
Study Notes
Ultra Microtome
- Used to cut ultrathin sections for transmission electron microscopy
- Cuts sections between 40 and 100 nanometric thickness
- Uses glass or diamond knife for cutting
Factors Involved in Cutting
- Lowering the temperature facilitates section cutting
- Higher rake angle helps in smooth flow of ribbons
- Lower rake angle is used for hard tissue
- Soft tissue is cut at a slower rate than hard tissue
Parts of Microtome
- Wheel Lock (A)
- Knife Stage (B)
- Knife Angle Locking Lever (C)
- Knife Locking Lever (D)
This quiz covers the basics of ultra microtome, including its use in transmission electron microscopy, factors involved in cutting, and its various parts. Topics include cutting techniques, knife angles, and tissue handling.
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