Types of RNA in Biology

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Questions and Answers

What is the primary function of ribosomal RNA (rRNA)?

  • Component of ribosome (correct)
  • Participate in mRNA editing
  • Template for protein synthesis
  • Transfer amino acids

Transfer RNA (tRNA) carries the genetic information from the nucleus to the ribosome.

False (B)

What is the role of the anticodon in transfer RNA (tRNA)?

To base-pair with the complementary code on the mRNA molecule

The 3' end of tRNA is known as the _____ attachment site.

<p>amino acid</p> Signup and view all the answers

Match the types of RNA with their functions:

<p>mRNA = Template for protein synthesis tRNA = Transfer amino acids rRNA = Component of ribosome snRNA = Participate in splicing</p> Signup and view all the answers

Which of the following types of small RNA induces degradation of targeted mRNA?

<p>microRNA (miRNA) (D)</p> Signup and view all the answers

Heterogeneous nuclear RNA (hnRNA) is considered premature mRNA.

<p>True (A)</p> Signup and view all the answers

What percentage of total cellular RNA is made up of Transfer RNA (tRNA)?

<p>10-20%</p> Signup and view all the answers

What is the total number of rRNA strands present in the larger subunit of a ribosome?

<p>2 (C)</p> Signup and view all the answers

Eukaryotic ribosomes are smaller than prokaryotic ribosomes.

<p>False (B)</p> Signup and view all the answers

What are the main nucleotide components of rRNA?

<p>Adenine, Guanine, Cytosine, and Uracil</p> Signup and view all the answers

The _____ site in a ribosome is where tRNA molecules attach during protein synthesis.

<p>A</p> Signup and view all the answers

Match the ribosome types to their size classification:

<p>Prokaryotic Ribosomes = 70S Eukaryotic Cytoplasmic Ribosomes = 80S Eukaryotic Mitochondrial Ribosomes = 55S Bacterial Ribosomes = Variable</p> Signup and view all the answers

Which of the following is NOT a feature of rRNA's secondary structure?

<p>Triple helical coils (D)</p> Signup and view all the answers

Both prokaryotic and eukaryotic ribosomes contain rRNA and proteins.

<p>True (A)</p> Signup and view all the answers

What role do ribosomes play in the cell?

<p>Protein synthesis</p> Signup and view all the answers

What is the primary function of mRNA?

<p>To carry genetic information about a protein sequence from DNA to ribosomes (A)</p> Signup and view all the answers

MRNA is a double-stranded molecule.

<p>False (B)</p> Signup and view all the answers

What are the major bases found in mRNA?

<p>A, G, C, U</p> Signup and view all the answers

Transcription is the process where mRNA is synthesized from __________.

<p>DNA</p> Signup and view all the answers

Match the following characteristics of mRNA with their descriptions:

<p>Very Heterogeneous = Wide range of molecular weights Abundance = Comprises about 5% of total RNA Location = Found in cytoplasm and mitochondria Post-Transcriptional Modifications = Include 5' capping and polyadenylation</p> Signup and view all the answers

What structural feature of mRNA stabilizes it and provides protection in the cytoplasm?

<p>5' cap with 7-methylguanosine (D)</p> Signup and view all the answers

Methylation typically occurs at the 5' terminal nucleoside of eukaryotic mRNAs.

<p>True (A)</p> Signup and view all the answers

What is a polyribosome?

<p>A linear aggregate formed by several ribosomes attached to a single mRNA molecule.</p> Signup and view all the answers

What unique feature of tRNA helps protect it from dehydration when in the cytoplasm?

<p>Methylation of unusual bases (B)</p> Signup and view all the answers

TRNA consists solely of the usual nitrogenous bases: adenine, uracil, guanine, and cytosine.

<p>False (B)</p> Signup and view all the answers

What are the two main legs of tRNA's L-shaped tertiary structure called?

<p>Acceptor and D stems, Anticodon and T stems</p> Signup and view all the answers

TRNA binds to specific amino acids and transfers them to the ________ ends of growing peptide chains.

<p>C-terminal</p> Signup and view all the answers

Match the unusual base with its corresponding conventional base:

<p>Inosine = Adenine Pseudouracil = Uracil Dihydroxyuridine = Uridine</p> Signup and view all the answers

What is the typical length of tRNA molecules?

<p>60-95 nucleotides (D)</p> Signup and view all the answers

Each tRNA molecule carries one Thymine (T) nucleotide.

<p>True (A)</p> Signup and view all the answers

What is formed when the $3^‘$-OH group of tRNA binds to the $eta$-COOH of a specific amino acid?

<p>aminoacyl-tRNA</p> Signup and view all the answers

The cloverleaf structure of tRNA is formed due to hydrogen bonding of complementary bases in different ______.

<p>segments</p> Signup and view all the answers

Which of the following is NOT a minor base found in tRNA?

<p>Cytosine (C) (C)</p> Signup and view all the answers

Match the structures of tRNA with their descriptions:

<p>Amino Acid Acceptor Arm = Binds the alpha-COOH of an amino acid Anticodon Stem = Complimentary to the mRNA codon T Stem = Contains T, pseudouridine, and C Variable Arm = Length varies from 3-23 nucleotides</p> Signup and view all the answers

The double helical stems of tRNA are typically in a B-DNA conformation.

<p>False (B)</p> Signup and view all the answers

What conformation do the double helical stems of tRNA resemble?

<p>A-DNA</p> Signup and view all the answers

What is the primary function of the 5' cap on eukaryotic mRNA?

<p>Serve as a recognition site for translation initiation (D)</p> Signup and view all the answers

Prokaryotic mRNA is typically monocistronic.

<p>False (B)</p> Signup and view all the answers

What is the main component of the poly-A tail added to eukaryotic mRNA?

<p>Adenine nucleotides</p> Signup and view all the answers

The precursor of mRNA in mammalian nuclei is called ______.

<p>hnRNA</p> Signup and view all the answers

Match the following types of RNA with their functions:

<p>mRNA = Contains the genetic code for proteins hnRNA = Precursor of mature mRNA snRNA = Involved in RNA processing poly-A tail = Stabilizes mRNA and aids export from the nucleus</p> Signup and view all the answers

How is the 5' cap connected to the 5' terminal nucleoside in eukaryotic mRNA?

<p>By a 5'-5' triphosphate bridge (B)</p> Signup and view all the answers

75% of hnRNA is processed into mature mRNA in the nucleus.

<p>False (B)</p> Signup and view all the answers

What is the role of U1 snRNA in RNA processing?

<p>Assisting in mRNA processing</p> Signup and view all the answers

Flashcards

mRNA

Carries genetic instructions from DNA to ribosomes for protein synthesis.

tRNA

A type of RNA that carries specific amino acids to the ribosome during protein synthesis.

rRNA

Component of ribosomes, where proteins are assembled.

hnRNA

Precursor messenger RNA; needs modification before it becomes mRNA.

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tRNA anticodon

A sequence of three nucleotides on tRNA that is complementary to a codon on mRNA, allowing the tRNA to match with the correct amino acid.

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Amino acid attachment site

The 3' end of tRNA where the specific amino acid is attached.

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Ribosome

A complex structure composed of rRNA and protein that carries out protein synthesis.

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Protein synthesis

The process of creating proteins from amino acids, using instructions from mRNA and performed by ribosomes.

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rRNA

Ribosomal RNA; a component of ribosomes, involved in protein synthesis.

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Ribosome

A complex molecular machine responsible for protein synthesis, composed of rRNA and proteins.

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Prokaryotic Ribosome

70S ribosome, composed of a 30S and 50S subunit, prevalent in bacteria.

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Eukaryotic Ribosome

80S ribosome, found in eukaryotic cells, consists of a 40S and 60S subunit.

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rRNA Structure

rRNA has a complex structure with double helix stems, single-stranded loops and non-bonded bulges.

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Ribosome Subunits

Ribosomes are composed of multiple rRNA (and protein) subunits, that work together for protein production.

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Protein Synthesis

The process where ribosomes read mRNA to build polypeptide chains into proteins, a fundamental process in cells.

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rRNA nucleotides

Ribosomal RNA consists primarily of A, G, C, and U nucleotides.

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mRNA Function

mRNA carries genetic information from DNA to ribosomes to make proteins.

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Codon

Three nucleotides in mRNA that code for one amino acid.

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mRNA Structure

Single-stranded RNA molecule synthesized from DNA.

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Transcription

Process of making mRNA from DNA.

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Polyribosome/Polysome

Several ribosomes translating the same mRNA molecule simultaneously.

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mRNA Heterogeneity

mRNA molecules have different sizes.

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mRNA Abundance

mRNA makes up a small percentage of total RNA.

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mRNA Location

mRNA is mostly found in the cell cytoplasm.

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5' Capping

Addition of a special structure at the beginning of mRNA.

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mRNA 5' Cap Structure

The 5' Cap is a modified form of guanine with a methyl group, attached to the 5' end of the mRNA.

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tRNA structure

tRNA has an L-shaped tertiary structure, formed from base-paired stems arranged into perpendicular legs (acceptor/T stem and D/anticodon stems).

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tRNA Primary Structure

tRNA's sequence of nucleotides (A, G, C, U) and modified bases, essential for amino acid attachment.

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5' Cap Function

The 5' cap, a modified guanine nucleotide, protects mRNA from degradation and helps initiate translation.

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tRNA tertiary stabilization

Interactions of stacking, crosslinks, and non-base-pairing H-bonds stabilize tRNA's L shape.

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tRNA Secondary Structure

tRNA's cloverleaf form, created by complementary base pairing within the molecule, forming stems and loops.

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Monocistronic mRNA

Eukaryotic mRNA that codes for only one protein.

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tRNA Tertiary Structure

tRNA's overall L-shaped structure. Interactions of stems and loops make it.

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Polycistronic mRNA

Prokaryotic mRNA that codes for multiple proteins.

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Unusual tRNA bases

tRNA contains modified bases like inosine (I), pseudouracil (ψ), and dihydroxyuridine, in addition to the standard bases (A, U, G, C).

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Amino Acid Acceptor Arm

tRNA region where amino acids bind, allowing aminoacyl-tRNA formation.

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Poly-A Tail Function

A string of adenine nucleotides at the 3' end of mRNA that increases mRNA stability and helps it exit the nucleus.

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Unusual tRNA base importance

Unusual tRNA bases protect the tRNA against degradation by enzymes in the cytoplasm by methylation.

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rRNA prevalence

Ribosomal RNA (rRNA) makes up 60-80% of the total cellular RNA.

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D Stem and D Loop

tRNA region with modified bases, particularly dihydrouracil (D).

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hnRNA

Heterogeneous nuclear RNA; the initial, large RNA transcript of a gene that is processed to form mRNA.

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Anticodon

Sequence in tRNA that's complementary to mRNA codons, pairing with them during translation.

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snRNA

Small nuclear RNA molecules involved in RNA processing, particularly mRNA processing.

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rRNA association

Ribosomal RNA mostly associates with proteins to form ribosomes, which are vital for peptide synthesis.

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5' Cap Structure

A modified guanine nucleotide (methylguanisine) attached to the 5' end of the mRNA by a 5'-5' triphosphate bridge.

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tRNA role

tRNA molecules carry specific amino acids to the mRNA codons during protein synthesis and brings them to the growing peptide chain.

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Anticodon Stem and Anticodon Loop

The tRNA region that reads mRNA codons to specify appropriate amino acids.

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Variable arm

tRNA section of variable length (3-23 nucleotides).

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Svedberg units

Ribosomes and rRNA are measured in Svedberg (S) units that indicate their sedimentation rates in an ultracentrifuge.

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T Stem and T Loop

tRNA region containing the TψC sequence (Thymine-pseudouridine-Cytosine).

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mRNA Codon

Set of three mRNA nucleotides that specifies a specific amino acid.

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Study Notes

Types of RNA

  • In prokaryotic and eukaryotic organisms, three main classes of RNA molecules exist
    • Messenger RNA (mRNA)
    • Transfer RNA (t RNA)
    • Ribosomal RNA (r RNA)
    • Heterogeneous nuclear RNA (hnRNA)
  • Small RNAs also exist:
    • Small nuclear RNA (snRNA)
    • Micro RNA (miRNA)
    • Small interfering RNA (siRNA)
    • Non-coding RNA (ncRNA)

Significance

  • mRNA - Carries genetic formation of DNA for protein synthesis from nucleus to ribosome
  • rRNA - Combines with proteins to form ribosomes
  • tRNA - Acts as an adapter molecule. Carries amino acid and drops it on mRNA with its anticodon on mRNA
  • snRNAs - part of spliceosomes - that edit mRNAs

tRNA Characteristics

  • There are two ends in the tRNA:
    • 5' end
    • 3' end
  • The 3' end is where the amino acid is attached and it is called the amino acid attachment site.

Transfer RNA (tRNA) or Soluble RNA (srRNA)

  • Smaller than other RNAs (MW 18-28 KD)
  • 10-20% of the total cellular RNA
  • In cytosol and mitochondrial matrix
  • Average animal cells carry around 56 tRNAs
  • Named according to the specific amino acid they bind to and transfer, eg. phenylalanine mRNA (tRNAPhe)

mRNA Structure and Function

  • tRNA, like mRNA, is made in the nucleus and is used over and over again.
  • tRNA binds an amino acid at one end and has an anticodon at the other end.
  • The anticodon acts to base pair with the complementary code on the mRNA molecule, and delivers an amino acid to the ribosome.

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