Podcast
Questions and Answers
What is the primary function of ribosomal RNA (rRNA)?
What is the primary function of ribosomal RNA (rRNA)?
- Component of ribosome (correct)
- Participate in mRNA editing
- Template for protein synthesis
- Transfer amino acids
Transfer RNA (tRNA) carries the genetic information from the nucleus to the ribosome.
Transfer RNA (tRNA) carries the genetic information from the nucleus to the ribosome.
False (B)
What is the role of the anticodon in transfer RNA (tRNA)?
What is the role of the anticodon in transfer RNA (tRNA)?
To base-pair with the complementary code on the mRNA molecule
The 3' end of tRNA is known as the _____ attachment site.
The 3' end of tRNA is known as the _____ attachment site.
Match the types of RNA with their functions:
Match the types of RNA with their functions:
Which of the following types of small RNA induces degradation of targeted mRNA?
Which of the following types of small RNA induces degradation of targeted mRNA?
Heterogeneous nuclear RNA (hnRNA) is considered premature mRNA.
Heterogeneous nuclear RNA (hnRNA) is considered premature mRNA.
What percentage of total cellular RNA is made up of Transfer RNA (tRNA)?
What percentage of total cellular RNA is made up of Transfer RNA (tRNA)?
What is the total number of rRNA strands present in the larger subunit of a ribosome?
What is the total number of rRNA strands present in the larger subunit of a ribosome?
Eukaryotic ribosomes are smaller than prokaryotic ribosomes.
Eukaryotic ribosomes are smaller than prokaryotic ribosomes.
What are the main nucleotide components of rRNA?
What are the main nucleotide components of rRNA?
The _____ site in a ribosome is where tRNA molecules attach during protein synthesis.
The _____ site in a ribosome is where tRNA molecules attach during protein synthesis.
Match the ribosome types to their size classification:
Match the ribosome types to their size classification:
Which of the following is NOT a feature of rRNA's secondary structure?
Which of the following is NOT a feature of rRNA's secondary structure?
Both prokaryotic and eukaryotic ribosomes contain rRNA and proteins.
Both prokaryotic and eukaryotic ribosomes contain rRNA and proteins.
What role do ribosomes play in the cell?
What role do ribosomes play in the cell?
What is the primary function of mRNA?
What is the primary function of mRNA?
MRNA is a double-stranded molecule.
MRNA is a double-stranded molecule.
What are the major bases found in mRNA?
What are the major bases found in mRNA?
Transcription is the process where mRNA is synthesized from __________.
Transcription is the process where mRNA is synthesized from __________.
Match the following characteristics of mRNA with their descriptions:
Match the following characteristics of mRNA with their descriptions:
What structural feature of mRNA stabilizes it and provides protection in the cytoplasm?
What structural feature of mRNA stabilizes it and provides protection in the cytoplasm?
Methylation typically occurs at the 5' terminal nucleoside of eukaryotic mRNAs.
Methylation typically occurs at the 5' terminal nucleoside of eukaryotic mRNAs.
What is a polyribosome?
What is a polyribosome?
What unique feature of tRNA helps protect it from dehydration when in the cytoplasm?
What unique feature of tRNA helps protect it from dehydration when in the cytoplasm?
TRNA consists solely of the usual nitrogenous bases: adenine, uracil, guanine, and cytosine.
TRNA consists solely of the usual nitrogenous bases: adenine, uracil, guanine, and cytosine.
What are the two main legs of tRNA's L-shaped tertiary structure called?
What are the two main legs of tRNA's L-shaped tertiary structure called?
TRNA binds to specific amino acids and transfers them to the ________ ends of growing peptide chains.
TRNA binds to specific amino acids and transfers them to the ________ ends of growing peptide chains.
Match the unusual base with its corresponding conventional base:
Match the unusual base with its corresponding conventional base:
What is the typical length of tRNA molecules?
What is the typical length of tRNA molecules?
Each tRNA molecule carries one Thymine (T) nucleotide.
Each tRNA molecule carries one Thymine (T) nucleotide.
What is formed when the $3^‘$-OH group of tRNA binds to the $eta$-COOH of a specific amino acid?
What is formed when the $3^‘$-OH group of tRNA binds to the $eta$-COOH of a specific amino acid?
The cloverleaf structure of tRNA is formed due to hydrogen bonding of complementary bases in different ______.
The cloverleaf structure of tRNA is formed due to hydrogen bonding of complementary bases in different ______.
Which of the following is NOT a minor base found in tRNA?
Which of the following is NOT a minor base found in tRNA?
Match the structures of tRNA with their descriptions:
Match the structures of tRNA with their descriptions:
The double helical stems of tRNA are typically in a B-DNA conformation.
The double helical stems of tRNA are typically in a B-DNA conformation.
What conformation do the double helical stems of tRNA resemble?
What conformation do the double helical stems of tRNA resemble?
What is the primary function of the 5' cap on eukaryotic mRNA?
What is the primary function of the 5' cap on eukaryotic mRNA?
Prokaryotic mRNA is typically monocistronic.
Prokaryotic mRNA is typically monocistronic.
What is the main component of the poly-A tail added to eukaryotic mRNA?
What is the main component of the poly-A tail added to eukaryotic mRNA?
The precursor of mRNA in mammalian nuclei is called ______.
The precursor of mRNA in mammalian nuclei is called ______.
Match the following types of RNA with their functions:
Match the following types of RNA with their functions:
How is the 5' cap connected to the 5' terminal nucleoside in eukaryotic mRNA?
How is the 5' cap connected to the 5' terminal nucleoside in eukaryotic mRNA?
75% of hnRNA is processed into mature mRNA in the nucleus.
75% of hnRNA is processed into mature mRNA in the nucleus.
What is the role of U1 snRNA in RNA processing?
What is the role of U1 snRNA in RNA processing?
Flashcards
mRNA
mRNA
Carries genetic instructions from DNA to ribosomes for protein synthesis.
tRNA
tRNA
A type of RNA that carries specific amino acids to the ribosome during protein synthesis.
rRNA
rRNA
Component of ribosomes, where proteins are assembled.
hnRNA
hnRNA
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tRNA anticodon
tRNA anticodon
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Amino acid attachment site
Amino acid attachment site
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Ribosome
Ribosome
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Protein synthesis
Protein synthesis
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rRNA
rRNA
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Ribosome
Ribosome
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Prokaryotic Ribosome
Prokaryotic Ribosome
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Eukaryotic Ribosome
Eukaryotic Ribosome
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rRNA Structure
rRNA Structure
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Ribosome Subunits
Ribosome Subunits
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Protein Synthesis
Protein Synthesis
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rRNA nucleotides
rRNA nucleotides
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mRNA Function
mRNA Function
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Codon
Codon
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mRNA Structure
mRNA Structure
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Transcription
Transcription
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Polyribosome/Polysome
Polyribosome/Polysome
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mRNA Heterogeneity
mRNA Heterogeneity
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mRNA Abundance
mRNA Abundance
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mRNA Location
mRNA Location
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5' Capping
5' Capping
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mRNA 5' Cap Structure
mRNA 5' Cap Structure
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tRNA structure
tRNA structure
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tRNA Primary Structure
tRNA Primary Structure
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5' Cap Function
5' Cap Function
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tRNA tertiary stabilization
tRNA tertiary stabilization
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tRNA Secondary Structure
tRNA Secondary Structure
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Monocistronic mRNA
Monocistronic mRNA
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tRNA Tertiary Structure
tRNA Tertiary Structure
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Polycistronic mRNA
Polycistronic mRNA
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Unusual tRNA bases
Unusual tRNA bases
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Amino Acid Acceptor Arm
Amino Acid Acceptor Arm
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Poly-A Tail Function
Poly-A Tail Function
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Unusual tRNA base importance
Unusual tRNA base importance
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rRNA prevalence
rRNA prevalence
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D Stem and D Loop
D Stem and D Loop
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hnRNA
hnRNA
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Anticodon
Anticodon
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snRNA
snRNA
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rRNA association
rRNA association
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5' Cap Structure
5' Cap Structure
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tRNA role
tRNA role
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Anticodon Stem and Anticodon Loop
Anticodon Stem and Anticodon Loop
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Variable arm
Variable arm
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Svedberg units
Svedberg units
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T Stem and T Loop
T Stem and T Loop
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mRNA Codon
mRNA Codon
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Study Notes
Types of RNA
- In prokaryotic and eukaryotic organisms, three main classes of RNA molecules exist
- Messenger RNA (mRNA)
- Transfer RNA (t RNA)
- Ribosomal RNA (r RNA)
- Heterogeneous nuclear RNA (hnRNA)
- Small RNAs also exist:
- Small nuclear RNA (snRNA)
- Micro RNA (miRNA)
- Small interfering RNA (siRNA)
- Non-coding RNA (ncRNA)
Significance
- mRNA - Carries genetic formation of DNA for protein synthesis from nucleus to ribosome
- rRNA - Combines with proteins to form ribosomes
- tRNA - Acts as an adapter molecule. Carries amino acid and drops it on mRNA with its anticodon on mRNA
- snRNAs - part of spliceosomes - that edit mRNAs
tRNA Characteristics
- There are two ends in the tRNA:
- 5' end
- 3' end
- The 3' end is where the amino acid is attached and it is called the amino acid attachment site.
Transfer RNA (tRNA) or Soluble RNA (srRNA)
- Smaller than other RNAs (MW 18-28 KD)
- 10-20% of the total cellular RNA
- In cytosol and mitochondrial matrix
- Average animal cells carry around 56 tRNAs
- Named according to the specific amino acid they bind to and transfer, eg. phenylalanine mRNA (tRNAPhe)
mRNA Structure and Function
- tRNA, like mRNA, is made in the nucleus and is used over and over again.
- tRNA binds an amino acid at one end and has an anticodon at the other end.
- The anticodon acts to base pair with the complementary code on the mRNA molecule, and delivers an amino acid to the ribosome.
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