Types of Microscopes in Human Physiology Lab.1
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Questions and Answers

What is the function of a microscope?

  • To reduce the size of small objects
  • To change the color of small objects
  • To magnify small objects that are too small to be seen by the naked eye (correct)
  • To increase the weight of small objects

    • What is the most widely used type of microscope?

  • Bright Field Microscope
  • Light Microscope (correct)
  • Compound Microscope
  • Electron Microscope

    • What is the main difference between Light Microscope and Electron Microscope?

  • Light Microscope has one lens, while Electron Microscope has multiple lenses
  • Light Microscope uses light to produce images, while Electron Microscope uses beams of electrons (correct)
  • Light Microscope is used for viruses, DNA, and parts of cells, while Electron Microscope is used for simple organisms
  • Light Microscope produces smaller images than Electron Microscope

    • What is the function of the fine adjustment knob on a microscope?

    <p>To up or down the stage movement when focusing the medium and high power objective lenses</p> Signup and view all the answers

    What does a compound microscope have that a simple microscope does not?

    <p>2 or more lenses</p> Signup and view all the answers

    What supports the upper parts of a microscope and is used for carrying the microscope?

    <p>Arm (Handle, Limb)</p> Signup and view all the answers

    What is the function of the condenser in a microscope?

    <p>To adjust the amount of light reaching the eye</p> Signup and view all the answers

    How is total magnification calculated in a microscope?

    <p>By multiplying the power of the objective lens by the power of the eyepiece lens</p> Signup and view all the answers

    What is the function of the stage clips in a microscope?

    <p>To secure the specimen slide in place on the stage</p> Signup and view all the answers

    What should be done before using the oil immersion lens in a microscope?

    <p>Place a drop of oil over the specimen</p> Signup and view all the answers

    Which part of the microscope connects the eyepiece to the objective lenses?

    <p>Body tube</p> Signup and view all the answers

    What is the role of the diaphragm in a microscope?

    <p>To control the amount of light that reaches your eye</p> Signup and view all the answers

    In a microscope, what is meant by resolution?

    <p>Power of the microscope to show detail clearly</p> Signup and view all the answers

    What is denoted by 'X' when referring to magnification in microscopes?

    <p>'X' signifies measuring magnifying ability</p> Signup and view all the answers

    What is meant by 'stage control' in relation to microscopes?

    <p>Stage control refers to moving stage left and right</p> Signup and view all the answers

    What should be done after connecting a light microscope to an outlet?

    <p>Adjust condenser and diaphragm</p> Signup and view all the answers

    What is the function of the fine adjustment knob on a microscope?

    <p>To up or down the stage movement when focusing the medium and high power objective lenses.</p> Signup and view all the answers

    What does a compound microscope have that a simple microscope does not?

    <p>Two or more lenses.</p> Signup and view all the answers

    What is the main difference between a light microscope and an electron microscope?

    <p>The main difference is that an electron microscope uses beams of electrons, while a light microscope uses light to produce images.</p> Signup and view all the answers

    What is the support system of a microscope?

    <p>Arm (Handle, Limb) and Base.</p> Signup and view all the answers

    What is the function of the condenser in a microscope?

    <p>To focus light onto the specimen.</p> Signup and view all the answers

    What is the function of the course adjustment knob on a microscope?

    <p>To move the stage up or down when focusing with the low power objective lens.</p> Signup and view all the answers

    What is the function of the stage clips in a microscope?

    <p>To hold the specimen slide in place on the stage.</p> Signup and view all the answers

    What is the purpose of the condenser in a microscope?

    <p>To focus the light on the specimen.</p> Signup and view all the answers

    What should be done before using the oil immersion lens in a microscope?

    <p>Place a drop of oil over the specimen.</p> Signup and view all the answers

    What is the total magnification if the objective lens is forty times (40X) and the eyepiece lens is ten times (10X)?

    <p>400X.</p> Signup and view all the answers

    How is the light focused on the specimen in a microscope?

    <p>Through the condenser.</p> Signup and view all the answers

    What is the main function of the diaphragm in a microscope?

    <p>To control the amount of light that reaches the eye.</p> Signup and view all the answers

    What should be done after placing a slide on the stage of a microscope?

    <p>Secure it with stage clips.</p> Signup and view all the answers

    How is the total magnification of an object calculated in a microscope?

    <p>By multiplying the eyepiece lens magnification by the objective lens magnification.</p> Signup and view all the answers

    What is the function of the nose piece in a microscope?

    <p>To hold the objective lenses and increase the magnification by turning.</p> Signup and view all the answers

    What is the role of the stage control in a microscope?

    <p>To move the stage left and right.</p> Signup and view all the answers

    Study Notes

    Microscope Functions and Components

    • Microscopes are instruments used to magnify and resolve fine details of small objects, enabling detailed observation beyond the resolution of the naked eye.
    • The most widely used type of microscope is the compound microscope, which employs multiple lenses to achieve high levels of magnification.
    • A Light Microscope uses visible light to illuminate specimens, while an Electron Microscope utilizes electron beams for higher resolution and magnification.
    • The fine adjustment knob is used for precise focusing of the specimen, allowing for a clearer view at high magnifications.

    Compound vs. Simple Microscope

    • A compound microscope features multiple lenses (objective and eyepiece) for greater magnification, whereas a simple microscope has only one lens.
    • The arm of the microscope supports the upper parts and provides a means for carrying the instrument.

    Optical Components and Functionality

    • The condenser focuses light onto the specimen, improving illumination and contrast for better visualization.
    • Total magnification is calculated by multiplying the magnification of the objective lens by the magnification of the eyepiece lens.
    • Stage clips hold the slide in place during observation to prevent movement.

    Preparation and Usage

    • Before using the oil immersion lens, a special immersion oil should be applied to reduce light scattering and enhance clarity.
    • The body tube connects the eyepiece to the objective lenses, allowing light to travel from the specimen to the observer’s eye.
    • The diaphragm regulates the amount of light reaching the specimen, impacting the overall image quality.

    Key Microscope Terminology

    • Resolution refers to the microscope's ability to distinguish two close objects as separate entities.
    • Magnification is denoted by 'X', indicating how many times larger the specimen appears compared to its actual size.
    • Stage control allows precise movement of the slide horizontally and vertically for proper positioning under the lens.

    Procedure and Maintenance

    • After connecting a light microscope to an outlet, the light source must be turned on to illuminate the specimen.
    • Ensure the slide is placed securely on the stage before beginning observation.
    • The coarse adjustment knob provides rough focusing adjustments, typically used at lower magnifications.

    Summary of Procedures

    • The total magnification with a 40X objective and a 10X eyepiece is 400X.
    • Light is focused on the specimen through the condenser system, increasing the detail observable.
    • After placing a slide on the stage, additional adjustments may be necessary using the stage control to focus on the desired area.

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    Description

    Learn about different types of microscopes used in human physiology lab, including light microscopes and electron microscopes. Understand how these instruments magnify small objects that are too small to be seen by the naked eye.

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