30 Questions
What direction does the air flow in a horizontal flow hood?
Parallel to the work surface
Which type of flow hood provides significant protection to the user and the cell culture?
Vertical flow hood
What is the primary function of a clean bench?
To provide product protection
What is the purpose of a biosafety cabinet?
To provide protection to the user and the cell culture
Where can information on the selection and use of biosafety cabinets be found?
When should clean benches be used?
When assembling sterile equipment
What type of objective lens was used to obtain the images?
100/1.3
What is a consequence of using antibiotics routinely in cell culture?
It encourages the development of antibiotic resistant strains
What is the purpose of periodically checking the characteristics of cell lines?
To avoid cross-contamination
What method(s) can be used to confirm the presence or absence of cross-contamination?
All of the above
Why should antibiotics not be used as a first resort in cell culture?
All of the above
What is the recommended use of antibiotics in cell culture?
Use them only as a last resort and for short-term applications
What is the typical shape of epithelial-like cells?
Polygonal
Neuronal cells are classified into how many basic morphological categories?
Two
What is the dendritic tree?
Cellular extensions with many branches from the cell body
What is the 293 cell line transformed with?
Sheared human adenovirus type 5 DNA
What is the advantage of 293 cell line?
Producing high levels of recombinant proteins
Lymphoblast-like cells are typically grown in?
Suspension without attaching to a surface
What is the first step in subculturing adherent cells?
Detaching them from the surface of the culture vessel
What is the purpose of a cell scraper in cell dissociation procedures?
To detach cell lines sensitive to proteases
What is the application of trypsin in cell dissociation procedures?
Detaching strongly adherent cells
What is the function of Dispase in cell dissociation procedures?
Detaching epidermal cells as confluent, intact sheets
What is the difference between TrypLE™ and trypsin?
TrypLE™ is an animal origin-free alternative to trypsin
Why is it recommended to optimize the incubation time for dissociation when using TrypLE™ enzymes?
To ensure the best results are obtained
What is the main reason TrypLE™ enzymes are preferred in certain applications?
They are animal origin-free reagents
How do TrypLE™ Express and TrypLE™ Select differ from trypsin in terms of storage?
They are stable at room temperature for at least six months
What is the consequence of deviating from the culture conditions required for a particular cell type?
The expression of aberrant phenotypes or a complete failure of the cell culture
What is the recommended approach when passaging your own cell line?
Follow the instructions provided with each product
What is the difference between passaging mammalian cells and insect cells?
The protocol for passaging insect cells differs from that for mammalian cells on several crucial steps
Why is it important to follow the instructions provided with each product when passaging your own cell line?
To avoid the consequences of deviating from the culture conditions required for a particular cell type
Learn about the differences between horizontal and vertical flow hoods, including their design and purpose. Understand how they provide protection to the user and the culture. Test your knowledge of laboratory equipment and safety protocols.
Make Your Own Quizzes and Flashcards
Convert your notes into interactive study material.
Get started for free
