Podcast
Questions and Answers
What direction does the air flow in a horizontal flow hood?
What direction does the air flow in a horizontal flow hood?
- Away from the work surface
- Diagonally, from the side of the cabinet
- Parallel to the work surface (correct)
- Vertically, from the top of the cabinet
Which type of flow hood provides significant protection to the user and the cell culture?
Which type of flow hood provides significant protection to the user and the cell culture?
- Clean bench
- Horizontal flow hood
- Vertical flow hood (correct)
- Laminar flow cabinet
What is the primary function of a clean bench?
What is the primary function of a clean bench?
- To manipulate infectious materials
- To provide product protection (correct)
- To provide protection to the user only
- To provide protection to the user and the cell culture
What is the purpose of a biosafety cabinet?
What is the purpose of a biosafety cabinet?
Where can information on the selection and use of biosafety cabinets be found?
Where can information on the selection and use of biosafety cabinets be found?
When should clean benches be used?
When should clean benches be used?
What type of objective lens was used to obtain the images?
What type of objective lens was used to obtain the images?
What is a consequence of using antibiotics routinely in cell culture?
What is a consequence of using antibiotics routinely in cell culture?
What is the purpose of periodically checking the characteristics of cell lines?
What is the purpose of periodically checking the characteristics of cell lines?
What method(s) can be used to confirm the presence or absence of cross-contamination?
What method(s) can be used to confirm the presence or absence of cross-contamination?
Why should antibiotics not be used as a first resort in cell culture?
Why should antibiotics not be used as a first resort in cell culture?
What is the recommended use of antibiotics in cell culture?
What is the recommended use of antibiotics in cell culture?
What is the typical shape of epithelial-like cells?
What is the typical shape of epithelial-like cells?
Neuronal cells are classified into how many basic morphological categories?
Neuronal cells are classified into how many basic morphological categories?
What is the dendritic tree?
What is the dendritic tree?
What is the 293 cell line transformed with?
What is the 293 cell line transformed with?
What is the advantage of 293 cell line?
What is the advantage of 293 cell line?
Lymphoblast-like cells are typically grown in?
Lymphoblast-like cells are typically grown in?
What is the first step in subculturing adherent cells?
What is the first step in subculturing adherent cells?
What is the purpose of a cell scraper in cell dissociation procedures?
What is the purpose of a cell scraper in cell dissociation procedures?
What is the application of trypsin in cell dissociation procedures?
What is the application of trypsin in cell dissociation procedures?
What is the function of Dispase in cell dissociation procedures?
What is the function of Dispase in cell dissociation procedures?
What is the difference between TrypLE™ and trypsin?
What is the difference between TrypLE™ and trypsin?
Why is it recommended to optimize the incubation time for dissociation when using TrypLE™ enzymes?
Why is it recommended to optimize the incubation time for dissociation when using TrypLE™ enzymes?
What is the main reason TrypLE™ enzymes are preferred in certain applications?
What is the main reason TrypLE™ enzymes are preferred in certain applications?
How do TrypLE™ Express and TrypLE™ Select differ from trypsin in terms of storage?
How do TrypLE™ Express and TrypLE™ Select differ from trypsin in terms of storage?
What is the consequence of deviating from the culture conditions required for a particular cell type?
What is the consequence of deviating from the culture conditions required for a particular cell type?
What is the recommended approach when passaging your own cell line?
What is the recommended approach when passaging your own cell line?
What is the difference between passaging mammalian cells and insect cells?
What is the difference between passaging mammalian cells and insect cells?
Why is it important to follow the instructions provided with each product when passaging your own cell line?
Why is it important to follow the instructions provided with each product when passaging your own cell line?
