Types of Laminar Flow Hoods

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What direction does the air flow in a horizontal flow hood?

Away from the work surface

Diagonally, from the side of the cabinet

Parallel to the work surface (correct)

Vertically, from the top of the cabinet

Which type of flow hood provides significant protection to the user and the cell culture?

Clean bench

Horizontal flow hood

Vertical flow hood (correct)

Laminar flow cabinet

What is the primary function of a clean bench?

To manipulate infectious materials

To provide product protection (correct)

To provide protection to the user only

To provide protection to the user and the cell culture

What is the purpose of a biosafety cabinet?

To provide protection to the user and the cell culture Signup and view all the answers

Where can information on the selection and use of biosafety cabinets be found?

<a href="http://www.cdc.gov/od/ohs/biosfty/bmbl5/bmbl5toc.htm">www.cdc.gov/od/ohs/biosfty/bmbl5/bmbl5toc.htm</a> Signup and view all the answers

When should clean benches be used?

When assembling sterile equipment Signup and view all the answers

What type of objective lens was used to obtain the images?

100/1.3 Signup and view all the answers

What is a consequence of using antibiotics routinely in cell culture?

It encourages the development of antibiotic resistant strains Signup and view all the answers

What is the purpose of periodically checking the characteristics of cell lines?

To avoid cross-contamination Signup and view all the answers

What method(s) can be used to confirm the presence or absence of cross-contamination?

All of the above Signup and view all the answers

Why should antibiotics not be used as a first resort in cell culture?

All of the above Signup and view all the answers

What is the recommended use of antibiotics in cell culture?

Use them only as a last resort and for short-term applications Signup and view all the answers

What is the typical shape of epithelial-like cells?

Polygonal Signup and view all the answers

Neuronal cells are classified into how many basic morphological categories?

Two Signup and view all the answers

What is the dendritic tree?

Cellular extensions with many branches from the cell body Signup and view all the answers

What is the 293 cell line transformed with?

Sheared human adenovirus type 5 DNA Signup and view all the answers

What is the advantage of 293 cell line?

Producing high levels of recombinant proteins Signup and view all the answers

Lymphoblast-like cells are typically grown in?

Suspension without attaching to a surface Signup and view all the answers

What is the first step in subculturing adherent cells?

Detaching them from the surface of the culture vessel Signup and view all the answers

What is the purpose of a cell scraper in cell dissociation procedures?

To detach cell lines sensitive to proteases Signup and view all the answers

What is the application of trypsin in cell dissociation procedures?

Detaching strongly adherent cells Signup and view all the answers

What is the function of Dispase in cell dissociation procedures?

Detaching epidermal cells as confluent, intact sheets Signup and view all the answers

What is the difference between TrypLE™ and trypsin?

TrypLE™ is an animal origin-free alternative to trypsin Signup and view all the answers

Why is it recommended to optimize the incubation time for dissociation when using TrypLE™ enzymes?

To ensure the best results are obtained Signup and view all the answers

What is the main reason TrypLE™ enzymes are preferred in certain applications?

They are animal origin-free reagents Signup and view all the answers

How do TrypLE™ Express and TrypLE™ Select differ from trypsin in terms of storage?

They are stable at room temperature for at least six months Signup and view all the answers

What is the consequence of deviating from the culture conditions required for a particular cell type?

The expression of aberrant phenotypes or a complete failure of the cell culture Signup and view all the answers

What is the recommended approach when passaging your own cell line?

Follow the instructions provided with each product Signup and view all the answers

What is the difference between passaging mammalian cells and insect cells?

The protocol for passaging insect cells differs from that for mammalian cells on several crucial steps Signup and view all the answers

Why is it important to follow the instructions provided with each product when passaging your own cell line?

To avoid the consequences of deviating from the culture conditions required for a particular cell type Signup and view all the answers