Podcast
Questions and Answers
What are the two recombinant proteins used to stimulate the release of interferon gamma in IGRA test systems?
What are the two recombinant proteins used to stimulate the release of interferon gamma in IGRA test systems?
ESAT6 and CFPT10
What is the primary limitation of IGRA test systems in population screening for tuberculosis?
What is the primary limitation of IGRA test systems in population screening for tuberculosis?
High cost and requirement for specialized laboratory equipment and personnel
What type of blood cells are isolated and evaluated in the ELISPOT (T-SPOT.TB) system?
What type of blood cells are isolated and evaluated in the ELISPOT (T-SPOT.TB) system?
Peripheral blood mononuclear cells
How do the ELISA quantifier and QuantiFERON coat TB golden tube systems differ from the ELISPOT system?
How do the ELISA quantifier and QuantiFERON coat TB golden tube systems differ from the ELISPOT system?
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What is a significant challenge in using IGRA test systems in pediatric populations?
What is a significant challenge in using IGRA test systems in pediatric populations?
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What is the primary mechanism by which IGRA test systems detect Mycobacterium tuberculosis and Mycobacterium bovis?
What is the primary mechanism by which IGRA test systems detect Mycobacterium tuberculosis and Mycobacterium bovis?
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What is the main purpose of incubating whole blood with recombinant proteins ESAT6 and CFPT10 in IGRA test systems?
What is the main purpose of incubating whole blood with recombinant proteins ESAT6 and CFPT10 in IGRA test systems?
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How do laboratory specialists evaluate the results of the ELISPOT (T-SPOT.TB) system?
How do laboratory specialists evaluate the results of the ELISPOT (T-SPOT.TB) system?
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What is a major limitation of IGRA test systems in terms of their practical application?
What is a major limitation of IGRA test systems in terms of their practical application?
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What is the primary reason why IGRA test systems are not suitable for population screening for tuberculosis?
What is the primary reason why IGRA test systems are not suitable for population screening for tuberculosis?
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Study Notes
Interferon-Gamma Release Assays (IGRA)
- International studies on the mycobacterium genome led to the development of two IGRA variants for TB diagnosis
- IGRA tests detect interferon-gamma release by activated memory T-cells in response to stimulation with recombinant proteins ESAT6 and CFP10
- Fresh blood is required for the analysis
ELISPOT (T-SPOT.TB) System
- Isolates peripheral blood mononuclear cells
- Evaluates the number of stain zones (visual or device-based)
- Sustains peripheral T-cells that release interferon-gamma after incubation with CFRT10 and ESAT6 recombinant proteins
ELISA QuantiFERON System
- Evaluates the concentration of interferon-gamma in blood plasma using enzyme-linked immunosorbent analysis
- Uses whole blood incubated with CFP10 and ESAT6 recombinant proteins
- QuantiFERON Coat TB Golden Tube is used for analysis
Limitations of IGRA Tests
- High cost
- Requires equipped laboratory and specialists
- Strict requirements for biological material preparation, storage, and blood processing
- Intravenous manipulation required in children
- Further studies needed to define their relevance in TB diagnostics
Interferon-Gamma Release Assays (IGRA)
- International studies on the mycobacterium genome led to the development of two IGRA variants for TB diagnosis
- IGRA tests detect interferon-gamma release by activated memory T-cells in response to stimulation with recombinant proteins ESAT6 and CFP10
- Fresh blood is required for the analysis
ELISPOT (T-SPOT.TB) System
- Isolates peripheral blood mononuclear cells
- Evaluates the number of stain zones (visual or device-based)
- Sustains peripheral T-cells that release interferon-gamma after incubation with CFRT10 and ESAT6 recombinant proteins
ELISA QuantiFERON System
- Evaluates the concentration of interferon-gamma in blood plasma using enzyme-linked immunosorbent analysis
- Uses whole blood incubated with CFP10 and ESAT6 recombinant proteins
- QuantiFERON Coat TB Golden Tube is used for analysis
Limitations of IGRA Tests
- High cost
- Requires equipped laboratory and specialists
- Strict requirements for biological material preparation, storage, and blood processing
- Intravenous manipulation required in children
- Further studies needed to define their relevance in TB diagnostics
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Description
Learn about the development and methodology of Interferon-Gamma Release Assays (IGRA) and the ELISPOT (T-SPOT.TB) System for diagnosing tuberculosis.
