Transfection Basics Quiz
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Questions and Answers

What is the purpose of inserting DNA into a cell during transfection?

  • To target the cytoplasm for protein production
  • To enable the expression of proteins using the cell's machinery (correct)
  • To incorporate DNA into the genomic DNA
  • To stop the translation of specific proteins

    • Where does the site of action for transfected RNA primarily occur?

  • Mitochondria
  • Nucleus
  • Cytoplasm (correct)
  • Endoplasmic reticulum

    • What happens to DNA after it is transported to the nucleus for effective transfection?

  • It can be transiently expressed or become incorporated into the genomic DNA (correct)
  • It is transported back to the cytoplasm
  • It remains in the nucleus without any further action
  • It is immediately degraded by the cell

    • Which of the following is NOT a commonly-used reagent for chemical mediated transfections?

    <p>Calcium carbonate</p> Signup and view all the answers

    What is the purpose of inserting double stranded RNA into a mammalian cell during transfection?

    <p>To down-regulate the production of a specific protein by stopping translation</p> Signup and view all the answers

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