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Transcription Initiation: RNA Polymerase and Sigma Subunit

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Transcription Initiation: RNA Polymerase and Sigma Subunit

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Questions and Answers

What is the role of eIF1 in the initiation of translation in eukaryotes?

binds to E site of small subunit

What is the function of eIF5B-GTP in the formation of the pre-initiation complex?

binds to complex to stabilize structure

What is the purpose of the helicase activity in the initiation of translation?

pulls mRNA through the ribosome

What is the role of eIF2-GTP in the formation of the pre-initiation complex?

brings initiator tRNA to bind to small subunit Signup and view all the answers

What is the difference between the initiation factor eIF4F in eukaryotes and its prokaryotic counterpart?

eIF4F binds to 5’cap and checks ends of mRNA Signup and view all the answers

What is the consequence of the hydrolysis of eIF2-GTP and eIF5B-GTP during the initiation of translation?

conformation change to 80S ribosome Signup and view all the answers

What is the role of eIF3 in the initiation of translation in eukaryotes?

binds to allosteric site of small subunit to prevent premature association Signup and view all the answers

What is the difference between the initiation of translation in prokaryotes and eukaryotes?

eukaryotes have different IFs, no shine-dalgaro sequence, and Met at initiator tRNA Signup and view all the answers

What is the role of the initiator protein in the initiation of translation in eukaryotes?

mRNA is added after the initiator protein Signup and view all the answers

What is the function of eIFA in the initiation of translation in eukaryotes?

binds to A site Signup and view all the answers