TLC Separation of Amino Acids
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Questions and Answers

What is the primary mechanism behind capillary action in TLC?

  • Gravitational forces acting on the solvent
  • Electrostatic attraction between the solvent and amino acids
  • Adhesion between the solvent and the stationary phase
  • Cohesion and surface tension of the solvent (correct)

    • What is the role of capillary action in TLC separation of amino acids?

  • To selectively retain amino acids on the stationary phase
  • To transport the solvent and amino acids up the TLC plate (correct)
  • To facilitate diffusion of amino acids across the TLC plate
  • To denature the amino acids for visualization

    • What is the result of capillary action on the TLC plate?

  • Amino acids are separated based on their molecular weight
  • Amino acids are separated based on their pKa values
  • Amino acids are separated based on their interaction with the stationary phase (correct)
  • Amino acids are separated based on their hydrophobicity

    • What is the effect of capillary action on the separation of amino acids?

    <p>It increases the resolution of the separated amino acids</p> Signup and view all the answers

    Why is capillary action important in TLC separation of amino acids?

    <p>It enables the separation of amino acids based on their interactions with the stationary phase</p> Signup and view all the answers

    Study Notes

    Capillary Action in TLC

    • Primary mechanism behind capillary action in TLC: surface tension and adsorption, where the solvent creeps up the thin layer of silica gel due to the attraction between the solvent molecules and the solid surface.

    Role of Capillary Action in TLC Separation of Amino Acids

    • Capillary action plays a crucial role in separating amino acids by carrying the solvent and the amino acids up the TLC plate, allowing them to interact with the stationary phase and separate based on their differing properties.

    Result of Capillary Action on the TLC Plate

    • As a result of capillary action, the solvent moves up the TLC plate, creating a uniform and controlled migration of the amino acids, allowing them to be separated and identified.

    Effect of Capillary Action on the Separation of Amino Acids

    • Capillary action enhances the separation of amino acids by allowing them to migrate at different rates, resulting in distinct and discrete bands on the TLC plate.

    Importance of Capillary Action in TLC Separation of Amino Acids

    • Capillary action is essential in TLC separation of amino acids as it enables the separation of these compounds based on their unique properties, allowing for accurate identification and analysis.

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    Description

    Explore the role of capillary action in Thin Layer Chromatography (TLC) for the separation of amino acids. Understand the primary mechanism behind capillary action and its effects on the TLC plate.

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