30 Questions
Which type of microscopy involves scanning the specimen at successive focal planes with a focused light beam, often from a laser, and produces a 3D reconstruction from the images?
Confocal microscopy
Which type of microscope is used in the study of living cells without the need for staining?
Phase contrast microscopy
What is the purpose of staining in microscopy?
To provide visual contrast and help identify specific tissue components
What is the main feature of electron microscopy?
Based on the interaction between electrons and tissue components
Which method of tissue preparation involves the use of sensitized photographic film to make non-visible light rays visible?
Autoradiography
What is the main focus of histology?
Studying how tissues are arranged to form organs
What is the purpose of fixation in tissue preparation for microscopic examination?
To preserve the molecular composition and structure of tissues
What is the purpose of a biopsy?
To remove tissues for diagnostic purposes
How does histology differ from autopsy?
Histology studies the arrangement of tissues, while autopsy examines the cause of death in a deceased body
Which major type of adult tissue is responsible for movement and locomotion?
Muscle
What is the purpose of tissue fixation in histology?
To avoid digestion and preserve the structure and molecular composition of tissues
Which type of microscopy allows recognition of highly organized structures such as cellulose, collagen, microtubules, and actin filaments?
Polarizing microscopy
What is the main feature of phase contrast microscopy?
It produces images without staining by utilizing differences in refractive index of natural cell and tissue components
What is the primary purpose of staining in microscopy?
To provide visual contrast and help identify specific tissue components
Which method is an alternative to chemical fixation for preserving tissues?
Freezing
What is the ideal purpose of microscopic tissue preparation?
To ensure the tissues have the same structure and molecular composition as in the body
What is the main focus of histology?
Understanding how tissues are arranged to form organs
Which major type of adult tissue is responsible for movement and locomotion?
Muscle
What is the purpose of a biopsy?
Removal of tissues for diagnostic purposes
Which method is used in the study of living cells without the need for staining?
Phase-contrast Microscopy
What is the primary purpose of tissue fixation in histology?
To preserve tissues for future examination
Which type of microscopy is used in the study of living cells without the need for staining?
Phase contrast microscopy
What is the main feature of electron microscopy?
It uses electrons to visualize ultrastructure
Which major type of adult tissue is responsible for movement and locomotion?
Muscle
What is the ideal purpose of microscopic tissue preparation?
To maintain the same structure and molecular composition as it has in the body
What is the purpose of tissue fixation in histology?
To preserve the structure and molecular composition of tissues
Which method is an alternative to chemical fixation for preserving tissues?
Freezing
What type of microscopy utilizes ultraviolet light to make only fluorescent molecules visible?
Confocal Microscopy
What is the primary purpose of staining in microscopy?
To provide visual contrast and identify specific tissue components
Which type of microscope involves scanning the specimen at successive focal planes with a focused light beam?
Confocal Microscopy
Study Notes
- Histology is the study of tissues in the body and their arrangement in organs, involving all aspects of tissue biology and optimization of functions for each organ.
- Tissues are collections of cells with similar structure and function, classified based on cell structure, non-cellular extracellular matrix, and cell function. Major types include epithelial, connective, muscle, and nervous tissue.
- Tissue examination for diagnostic and research purposes includes biopsy (removal of living tissues) and autopsy (post-mortem examination of organs).
- Tissues for microscopic examination need to maintain the same structure and molecular composition as in the body. Preparation steps include fixation (preservation using fixatives like formalin), embedding & sectioning (converting fixed tissue into a solid form for slicing), and staining (providing contrast for identification of tissue components).
- Light microscopes use visible light, while others use non-visible light sources. Light microscopy examines the interaction between light and tissue components, with methods including bright-field, fluorescence, phase contrast, confocal, and polarizing microscopy.
- Electron microscopes use electrons instead of light and include Transmission Electron Microscope (TEM) and Scanning Electron Microscope (SEM).
- Other tissue preparation methods include autoradiography, cell & tissue culture, enzyme histochemistry, and histochemistry.
- The study of histology and microscopy aims to equip students with the ability to define histology as a science, identify preparation techniques for microscopic examination, and understand the different types and applications of microscopy.
Learn about the study of tissues and how they make up organs, as well as procedures like biopsies and autopsies. This quiz provides an introduction to histology and the collection of cells with similar structures and functions.
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