Tissue Processing Techniques Quiz

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Sections can also be produced by means of crytostat or freezing microtome on frozen tissues. The fixed impregnated tissues have an advantage that they can be more easily stored and reproducibility of sections at a later date is easier

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Tissue processing refers to treatment of the tissue necessary to impregnate it into a solid medium so that the tissue is rendered sufficiently firm yet elastic for the tissue sections of desirable thickness to be cut on microtome

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Before proceeding on tissue processing as soon as the tissue is received it in very important that the tissue be properly labeled so as to avoid any confusion regarding duplication of same name or giving a wrong diagnosis to the patient

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The label should remain throughout the entire processing and later as permanent record keeping. To ensure this most laboratories have a numbering system for each specimen. As soon as the specimen is received it is given a specific individual number, which is also recorded in the register with the details like patient's name, name of the doctor referring it, nature of tissue is noted

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Labeling should not be done using ordinary ink as it gets dissolved in the reagent used during processing. Thin white card with a soft lead pencil, typed or printed labels are satisfactory

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Test your knowledge about the techniques and methods used for processing tissues for microscopic analysis, including impregnation, freezing, and sectioning.

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