Tissue Preparation and Fixation Quiz

Paraffin wax substitute correctly matched to its melting point?

The first and most important procedure in the preparation of tissues for microscopic examination is?

All are adhesives except:

Which of the following is not a clearing agent?

Which of the following is described as the reduction of body temperature and loss of skin elasticity that occurs after death?

What term refers to the ratio of speed of light in air and speed of light in a specific medium?

Who serves as the chairman of the council for Medical Technology Education?

Which is the best probable cause of this fixation problem?

Which of the following is used for the localization of vaginal adenosis?

Ideal volume or ratio of fixative to specimen?

This is the reversible change of one cell type to another?

What is the thickness of tissue paraffin sections that is the most ideal for routine histologic sectioning?

Tissue Preparation and Fixation

Paraffin wax substitutes and their melting points:
- Embeddol: 46-48°C
- Ester wax: 56-58°C
- Paraplast: 56-57°C (commonly used for embedding)
- Carbowax: 46-50°C
The most crucial step in tissue preparation for microscopic examination is fixation, which preserves cellular structure.
Common adhesives in tissue preparation:
- Glycerin jelly is not an adhesive.
- Other adhesives include gelatin, poly-L-lysine, and gelatin formaldehyde.
Clearing agents remove the embedding medium from the tissue:
- Xylene and clove oil are clearing agents.
- Acetone is not considered a clearing agent.
Algor mortis refers to the postmortem reduction of body temperature and loss of skin elasticity.
The refractive index defines the ratio of the speed of light in air to that in a specific medium, essential for optical evaluations.

Additional Information

The chairman of the council for Medical Technology Education is the CHED Secretary.
A common fixation failure, leading to tissue preparation problems, is the failure to fix specimens immediately.
The 4 quadrant vaginal scrape is utilized for localizing vaginal adenosis, a specific examination method.
The ideal fixative-to-specimen volume ratio is 20:1 for proper fixation.
Hyperplasia is described as the reversible change of one cell type to another, indicating a possible adjustment in tissue structure.
For routine histological sectioning, the optimal thickness of paraffin tissue sections is between 10-15 micrometers.

Test your knowledge on tissue preparation and fixation techniques essential for microscopic examination. This quiz covers the correct matching of paraffin wax substitutes and crucial procedures like fixation and embedding. Challenge yourself with questions on adhesives used in tissue preparation!