Tissue Fixation and Preparation Quiz

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Questions and Answers

What are the goals of tissue fixation?

  • Enhancing tissue coloration for better visual inspection (correct)
  • Preservation of tissue structure, prevention of decay, facilitating microscopic examination, preparation for molecular analysis and archiving (correct)
  • Facilitating the growth of microorganisms
  • Slowing down tissue metabolism and cellular activities (correct)

What are the characteristics of a good fixative?

  • Rapid tissue penetration, quick reaction, minimal structure alteration, safe to use, low cost (correct)
  • Variable tissue penetration, variable reaction speed, moderate structure alterations, variable safety, variable cost
  • No tissue penetration, no reaction, no structural alteration, not applicable for use, no cost
  • Slow tissue penetration, delayed reaction, significant structure alteration, dangerous to use, high cost

What is the purpose of the dehydration process in tissue preparation?

  • To increase the water content in tissues
  • To preserve tissue structure
  • To enhance tissue staining
  • To replace water and tissue with nonpolar substances (correct)

Flashcards

Goals of Tissue Fixation

Preservation of tissue structure, preventing decay, and facilitating microscopic examination.

Good Fixative Characteristics

Rapid penetration, quick reaction, minimal structure alteration, safe, and low cost.

Importance of pH in Formalin

Maintains stability to ensure effective tissue preservation.

Factors Affecting Tissue Fixation

Time and temperature are crucial, along with the type of material and fixative properties.

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Purpose of Dehydration

To replace water in tissues with non-polar substances.

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Intermediate Fluids Role

Facilitates the transition from dehydration to paraffin infiltration.

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Seminoma

The most common germ cell tumor of the testis.

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Benign Tumors

Characteristic for being restricted to the primary site of origin without metastasis.

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Chronic Inflammation Causes

May include infection, diabetes, and poor perfusion.

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Microscopic Features of RDS

Includes hyaline membranes in alveoli, atelectasis, and collapsed alveoli.

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Caseous Necrosis

A type of necrosis commonly seen in tuberculosis.

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Myocardial Infarction Characteristics

Is often associated with coagulative necrosis.

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Xeroderma Pigmentosum

An autosomal recessive disorder causing extreme sensitivity to UV light.

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Granulation Tissue Components

Includes fibroblasts, new capillaries, and inflammatory cells.

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Death Receptors

Surface molecules that initiate apoptosis upon binding to ligands.

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Thrombosis Cause

Often arises from damage to the vascular endothelium.

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Features of Pulmonary Embolism

Includes chest pain, shortness of breath, and altered consciousness.

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Prostate Cancer IHC Panel

Uses AMACR, CKMW, PSA, and p63 for diagnosis.

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Cushing's Ulcer Association

Commonly linked with brain injury or severe burns.

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Familial Mediterranean Fever

A hereditary condition causing recurrent fever and other symptoms.

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Grading Prostate Adenocarcinoma

Assesses nuclear morphology and tubular architecture.

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Pulmonary Congestion Features

Includes 'heart failure cells' and brownish discoloration of lungs.

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Sertoli Cell Tumor

A type of sex cord-stromal tumor.

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Langhans Cells Function

Act as antigen-presenting cells in inflammatory reactions.

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Characteristics of Malignant Tumors

Typically exhibit metastasis and rapid, invasive growth.

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Emboli Composition

Vast majority consists of thrombus material.

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Acute Inflammation Signs

Cardinal signs include redness, heat, swelling, pain, and loss of function.

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Clinical Diagnosis Process

Includes macroscopic assessment and considers clinical data.

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Study Notes

Tissue Fixation

  • Slows down tissue metabolism and cellular activities
  • Enhances tissue coloration for better visual inspection
  • Facilitates the growth of microorganisms
  • Preservation of tissue structure, prevention of decay, facilitating microscopic examination, preparation for molecular analysis and archiving

Good Fixative Characteristics

  • Rapid tissue penetration, quick reaction, minimal structural alteration, safe to use, low cost
  • Variable tissue penetration, variable reaction speed, moderate structural alterations, variable cost
  • No tissue penetration, no reaction, no structural alteration, not applicable for use, no cost

Tissue Preparation - Dehydration

  • To replace water in tissues with non-polar substances
  • To increase the water content in tissues
  • To preserve tissue structure
  • To enhance tissue staining

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