Tissue Dissociation Methods for Cell Culture

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What is the advantage of using the enzymatic method for tissue dissociation?

It reduces fibral connectivity and produces a high yield (correct)

It results in differentiation of cells over time

It is the safest and most consistent way

It leads to inconsistent results between users

Which chemical is commonly used in the chemical dissociation method to break cations between cells?

EDTA (correct)

Sodium hydroxide

Acetic acid

Hydrochloric acid

What is a disadvantage of using finite cell lines in tissue culture?

Maintenance of cellular characteristics of parent tissues

Predominance of cells with highest growth capacity (correct)

Differentiation of cells over time

Limited number of passages

Why may mechanical dissociation methods lead to inconsistent results between users?

Due to the amount of force applied resulting in different cell yields Signup and view all the answers

What effect does aberrant (abnormal) cell selection have during tissue culture assays?

May affect the accuracy of experimental results Signup and view all the answers

Which type of enzyme is commonly used to modify proteins on the cell surface for downstream assay alterations?

Trypsin Signup and view all the answers

What is a disadvantage of primary culture in terms of cell division?

Limited number of cell division leads to senescence Signup and view all the answers

Which factor makes primary culture difficult to culture?

Different environment to parent tissue Signup and view all the answers

What is a challenge associated with tumour primary cell culture in terms of metastasis?

Tumour cells can metastasize from the origin of proliferation Signup and view all the answers

Why are tumour primary cell cultures easier to maintain compared to non-cancerous cells?

Altered growth factors are released by tumour cells Signup and view all the answers

What makes it challenging to dissociate tissue for culturing in primary culture?

Senescence due to limited cell division Signup and view all the answers

Why is contamination a problem in primary cell cultures?

Different environment to parent tissue Signup and view all the answers

What is the purpose of a secondary or subculture of a cell line in tissue culture?

To add fresh media to aid cell growth Signup and view all the answers

What is the average time it takes for a cell population to double in size known as in tissue culture?

Population doubling time Signup and view all the answers

What is the main challenge associated with removing cells from an organ for cultivation in tissue culture?

Disruption of cell to cell and cell to membrane contacts Signup and view all the answers

Which method is used to dissociate tissue for culturing by enzymatic, mechanical, or chemical means?

Primary Culture Signup and view all the answers

Why are all removal steps of cells from an organ carried out in a cold environment?

To maintain in vitro and cell safety Signup and view all the answers

What does a primary cell line refer to in tissue culture?

Cells that survive the disaggregation process and attach to the culture vessel Signup and view all the answers