Podcast
Questions and Answers
What is the advantage of using the enzymatic method for tissue dissociation?
What is the advantage of using the enzymatic method for tissue dissociation?
- It reduces fibral connectivity and produces a high yield (correct)
- It results in differentiation of cells over time
- It is the safest and most consistent way
- It leads to inconsistent results between users
Which chemical is commonly used in the chemical dissociation method to break cations between cells?
Which chemical is commonly used in the chemical dissociation method to break cations between cells?
- EDTA (correct)
- Sodium hydroxide
- Acetic acid
- Hydrochloric acid
What is a disadvantage of using finite cell lines in tissue culture?
What is a disadvantage of using finite cell lines in tissue culture?
- Maintenance of cellular characteristics of parent tissues
- Predominance of cells with highest growth capacity (correct)
- Differentiation of cells over time
- Limited number of passages
Why may mechanical dissociation methods lead to inconsistent results between users?
Why may mechanical dissociation methods lead to inconsistent results between users?
What effect does aberrant (abnormal) cell selection have during tissue culture assays?
What effect does aberrant (abnormal) cell selection have during tissue culture assays?
Which type of enzyme is commonly used to modify proteins on the cell surface for downstream assay alterations?
Which type of enzyme is commonly used to modify proteins on the cell surface for downstream assay alterations?
What is a disadvantage of primary culture in terms of cell division?
What is a disadvantage of primary culture in terms of cell division?
Which factor makes primary culture difficult to culture?
Which factor makes primary culture difficult to culture?
What is a challenge associated with tumour primary cell culture in terms of metastasis?
What is a challenge associated with tumour primary cell culture in terms of metastasis?
Why are tumour primary cell cultures easier to maintain compared to non-cancerous cells?
Why are tumour primary cell cultures easier to maintain compared to non-cancerous cells?
What makes it challenging to dissociate tissue for culturing in primary culture?
What makes it challenging to dissociate tissue for culturing in primary culture?
Why is contamination a problem in primary cell cultures?
Why is contamination a problem in primary cell cultures?
What is the purpose of a secondary or subculture of a cell line in tissue culture?
What is the purpose of a secondary or subculture of a cell line in tissue culture?
What is the average time it takes for a cell population to double in size known as in tissue culture?
What is the average time it takes for a cell population to double in size known as in tissue culture?
What is the main challenge associated with removing cells from an organ for cultivation in tissue culture?
What is the main challenge associated with removing cells from an organ for cultivation in tissue culture?
Which method is used to dissociate tissue for culturing by enzymatic, mechanical, or chemical means?
Which method is used to dissociate tissue for culturing by enzymatic, mechanical, or chemical means?
Why are all removal steps of cells from an organ carried out in a cold environment?
Why are all removal steps of cells from an organ carried out in a cold environment?
What does a primary cell line refer to in tissue culture?
What does a primary cell line refer to in tissue culture?
