Podcast
Play an AI-generated podcast conversation about this lesson
Questions and Answers
What is the purpose of incubating the cells with daily observation till appearance of complete CPE?
What is the purpose of incubating the cells with daily observation till appearance of complete CPE?
- To confirm the presence of virus in the culture (correct)
- To ensure the virus is harvested at the right time
- To speed up the process of cell growth
- To prepare the cells for subculture
What is the key step to harvest the virus after 70% CPE?
What is the key step to harvest the virus after 70% CPE?
- Incubating at 37°C for 3-7 days
- Decanting the growth medium from the culture vessels
- Applying three cycles of freezing and thawing on infected cells (correct)
- Washing the cell monolayer with sterile PBS
What is the purpose of adding maintenance medium after removing the inoculum?
What is the purpose of adding maintenance medium after removing the inoculum?
- To provide nutrients for cell growth (correct)
- To wash the cell monolayer
- To prevent contamination
- To stimulate cell division
What is the significance of incubating the vessels at 37°C for 1-2 hours with gentle tilting every 10 minutes?
What is the significance of incubating the vessels at 37°C for 1-2 hours with gentle tilting every 10 minutes?
Signup and view all the answers
Why is it important to preserve the supernatant fluid containing the virus by ultra deep freezer?
Why is it important to preserve the supernatant fluid containing the virus by ultra deep freezer?
Signup and view all the answers
Flashcards are hidden until you start studying
