Tissue Culture Techniques-2 and CPE in Veterinary Medicine

To confirm the presence of virus in the culture (correct)

To ensure the virus is harvested at the right time

To speed up the process of cell growth

To prepare the cells for subculture

Incubating at 37°C for 3-7 days

Decanting the growth medium from the culture vessels

Applying three cycles of freezing and thawing on infected cells (correct)

Washing the cell monolayer with sterile PBS

To provide nutrients for cell growth (correct)

To wash the cell monolayer

To prevent contamination

To stimulate cell division

<p>To ensure even distribution of the virus on the cell monolayer</p> Signup and view all the answers

<p>To prevent degradation of the virus</p> Signup and view all the answers