5 Questions
What is the purpose of incubating the cells with daily observation till appearance of complete CPE?
To confirm the presence of virus in the culture
What is the key step to harvest the virus after 70% CPE?
Applying three cycles of freezing and thawing on infected cells
What is the purpose of adding maintenance medium after removing the inoculum?
To provide nutrients for cell growth
What is the significance of incubating the vessels at 37°C for 1-2 hours with gentle tilting every 10 minutes?
To ensure even distribution of the virus on the cell monolayer
Why is it important to preserve the supernatant fluid containing the virus by ultra deep freezer?
To prevent degradation of the virus
Test your knowledge of tissue culture techniques and CPE (CytoPathic Effect) in veterinary medicine with this quiz. Questions cover subculturing high-density suspended cells, inoculation of virus suspension, observation of CPE, and virus harvesting methods.
Make Your Own Quizzes and Flashcards
Convert your notes into interactive study material.
Get started for free
