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Questions and Answers
What is the purpose of applying three cycles of freezing and thawing on infected cells?
What is the purpose of applying three cycles of freezing and thawing on infected cells?
Why is it important to preserve the supernatant fluid containing the virus by using an ultra deep freezer?
Why is it important to preserve the supernatant fluid containing the virus by using an ultra deep freezer?
What is the purpose of incubating the vessels at 37ºC for 1-2 hours with gentle tilting every 10 minutes after adding the virus suspension to adherent cells?
What is the purpose of incubating the vessels at 37ºC for 1-2 hours with gentle tilting every 10 minutes after adding the virus suspension to adherent cells?
Why is it necessary to wash the cell monolayer with sterile PBS after removing the inoculum from adherent cells?
Why is it necessary to wash the cell monolayer with sterile PBS after removing the inoculum from adherent cells?
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What is the significance of daily observation until the appearance of complete CPE in suspended cells?
What is the significance of daily observation until the appearance of complete CPE in suspended cells?
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Study Notes
Virus Infection and Cell Culture
- Applying three cycles of freezing and thawing on infected cells helps to release the viral particles from the cells, allowing for efficient viral purification and concentration.
Virus Preservation
- Preserving the supernatant fluid containing the virus in an ultra-deep freezer is important to prevent viral degradation and inactivation, ensuring the virus remains viable for further experiments.
Virus Infection of Adherent Cells
- Incubating the vessels at 37ºC for 1-2 hours with gentle tilting every 10 minutes after adding the virus suspension to adherent cells facilitates the attachment and penetration of the virus into the cells, promoting efficient infection.
Cell Monolayer Preparation
- Washing the cell monolayer with sterile PBS after removing the inoculum from adherent cells is necessary to remove any residual virus and prevent further infection, ensuring a clean environment for cell growth and observation.
Monitoring Cytopathic Effects
- Daily observation until the appearance of complete Cytopathic Effects (CPE) in suspended cells is significant because it allows for the detection of viral replication and the assessment of the infectivity of the virus, enabling researchers to monitor the progression of the infection.
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Description
Test your knowledge of tissue culture techniques and Cytopathic Effect (CPE) in this quiz. Questions cover the subculture process, inoculation of virus suspension, observation of CPE, and virus harvesting methods.
