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Questions and Answers
What should be done to prevent excess of wax solidifying on the bottom of the block during winter?
What should be done if 2 or more tissues have to be casted?
What is the recommended approach for casting small biopsy fragments?
What should be done to facilitate easy removal of the block from the mould?
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What do whitish areas around tissue in a block denote?
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What is the primary advantage of using an automatic tissue processor?
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What is the purpose of the tissue containers in the automatic tissue processor?
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What is the function of the stirring mechanism in the automatic tissue processor?
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Why is a timer used in the automatic tissue processor?
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What is the purpose of wax baths in the automatic tissue processor?
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What is the recommended temperature setting for the wax bath thermostats?
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Why is it important to pay particular attention to fastening the processing baskets on carousel type machines?
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What should be done to prevent excess wax from solidifying on the bottom of the block during winter?
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What is the purpose of keeping watch on paraffin temperature?
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Why is it recommended to avoid leaving tissue in any solution for a long time?
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Study Notes
Preventing Wax Solidification
- To prevent excess wax from solidifying on the bottom of the block during winter, ensure the wax bath thermostat is set correctly.
Casting Multiple Tissues
- When casting 2 or more tissues, process them separately to prevent tissue fragments from getting mixed up.
Casting Small Biopsy Fragments
- For casting small biopsy fragments, use a specialized processing technique to ensure gentle handling and minimize tissue damage.
Removing the Block from the Mould
- To facilitate easy removal of the block from the mould, ensure the mould is properly coated with a release agent.
Whitish Areas Around Tissue
- Whitish areas around tissue in a block denote inadequate infiltration of wax or insufficient processing time.
Advantages of Automatic Tissue Processor
- The primary advantage of using an automatic tissue processor is increased efficiency and reduced processing time.
Tissue Containers
- The tissue containers in the automatic tissue processor are designed to hold and process multiple tissue samples simultaneously.
Stirring Mechanism
- The stirring mechanism in the automatic tissue processor ensures uniform distribution of reagents and solvents.
Timer Function
- The timer in the automatic tissue processor ensures precise control over processing times and temperatures.
Wax Baths
- The wax baths in the automatic tissue processor are used for infiltrating and embedding tissue samples in paraffin wax.
Recommended Temperature Setting
- The recommended temperature setting for the wax bath thermostats is between 56°C and 60°C.
Fastening Processing Baskets
- It is essential to pay particular attention to fastening the processing baskets on carousel type machines to prevent sample loss or contamination.
Preventing Wax Solidification
- To prevent excess wax from solidifying on the bottom of the block, maintain a consistent wax bath temperature and avoid overheating.
Monitoring Paraffin Temperature
- It is crucial to keep watch on paraffin temperature to ensure proper wax infiltration and embedding of tissue samples.
Solution Exposure Time
- It is recommended to avoid leaving tissue in any solution for a long time to prevent tissue damage and degradation.
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Description
Test your knowledge on the important points to consider during tissue casting, including the use of paraffin, prewarming moulds, and positioning of the tissue in the mould.
