Tissue Casting Procedure Precautions

Preventing Wax Solidification

• To prevent excess wax from solidifying on the bottom of the block during winter, ensure the wax bath thermostat is set correctly.

Casting Multiple Tissues

• When casting 2 or more tissues, process them separately to prevent tissue fragments from getting mixed up.

Casting Small Biopsy Fragments

• For casting small biopsy fragments, use a specialized processing technique to ensure gentle handling and minimize tissue damage.

Removing the Block from the Mould

• To facilitate easy removal of the block from the mould, ensure the mould is properly coated with a release agent.

Whitish Areas Around Tissue

• Whitish areas around tissue in a block denote inadequate infiltration of wax or insufficient processing time.

Advantages of Automatic Tissue Processor

• The primary advantage of using an automatic tissue processor is increased efficiency and reduced processing time.

Tissue Containers

• The tissue containers in the automatic tissue processor are designed to hold and process multiple tissue samples simultaneously.

Stirring Mechanism

• The stirring mechanism in the automatic tissue processor ensures uniform distribution of reagents and solvents.

Timer Function

• The timer in the automatic tissue processor ensures precise control over processing times and temperatures.

Wax Baths

• The wax baths in the automatic tissue processor are used for infiltrating and embedding tissue samples in paraffin wax.

Recommended Temperature Setting

• The recommended temperature setting for the wax bath thermostats is between 56°C and 60°C.

Fastening Processing Baskets

• It is essential to pay particular attention to fastening the processing baskets on carousel type machines to prevent sample loss or contamination.

Preventing Wax Solidification

• To prevent excess wax from solidifying on the bottom of the block, maintain a consistent wax bath temperature and avoid overheating.

Monitoring Paraffin Temperature

• It is crucial to keep watch on paraffin temperature to ensure proper wax infiltration and embedding of tissue samples.

Solution Exposure Time

• It is recommended to avoid leaving tissue in any solution for a long time to prevent tissue damage and degradation.

Test your knowledge on the important points to consider during tissue casting, including the use of paraffin, prewarming moulds, and positioning of the tissue in the mould.