The Scientific Method
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Questions and Answers

Which step of the scientific method involves making an educated guess based on research?

  • Hypothesis (correct)
  • Observation
  • Experiment
  • Question
  • What is the function of control variables in an experiment?

  • To measure the dependent variable
  • To observe the control group
  • To manipulate the independent variable
  • To provide unbiased results (correct)
  • Which type of variable varies from individual to individual?

  • Independent variable
  • Dependent variable (correct)
  • Control variable
  • None of the above
  • What is the purpose of a control group in an experiment?

    <p>To provide a baseline for comparison</p> Signup and view all the answers

    Why is sample size important in an experiment?

    <p>To obtain reliable research findings</p> Signup and view all the answers

    Which test is used to detect the presence of lipids in a sample?

    <p>Sudan IV test</p> Signup and view all the answers

    What is the function of the objective lens on a microscope?

    <p>To magnify the specimen</p> Signup and view all the answers

    What is the equation for calculating the field of view (FOV) of a microscope?

    <p>FOV 10x = FOV 4x x total mag 4x</p> Signup and view all the answers

    What is the function of the iris diaphragm on a microscope?

    <p>To control the amount of light</p> Signup and view all the answers

    What is the function of enzymes in our bodies?

    <p>To speed up metabolism</p> Signup and view all the answers

    Where does the Krebs cycle take place?

    <p>Mitochondrial matrix</p> Signup and view all the answers

    What is the purpose of sodium fluoride in cellular respiration?

    <p>Inhibits glycolysis</p> Signup and view all the answers

    What is the purpose of paper chromatography?

    <p>Separating pigments</p> Signup and view all the answers

    What is the equation for photosynthesis?

    <p>6CO2 + 6H2O → C6H12O6+ 6O</p> Signup and view all the answers

    What is the equation for cellular respiration?

    <p>C6H12O6 + 6O2 → 6CO2 + 6H2O</p> Signup and view all the answers

    True or false: The hypothesis is often written using the words 'IF' and 'THEN'.

    <p>True</p> Signup and view all the answers

    True or false: Controls are essential for unbiased experiments.

    <p>True</p> Signup and view all the answers

    True or false: The amount of water given to the plants is the independent variable.

    <p>True</p> Signup and view all the answers

    True or false: Sample size directly influences research findings.

    <p>True</p> Signup and view all the answers

    True or false: Carbohydrates are the first class of organic molecules.

    <p>True</p> Signup and view all the answers

    True or false: Nucleic acids are considered organic molecules.

    <p>True</p> Signup and view all the answers

    True or false: Benedict's test is used to detect proteins.

    <p>False</p> Signup and view all the answers

    True or false: Biuret test is used to detect simple carbohydrates.

    <p>False</p> Signup and view all the answers

    True or false: Iodine test is used to detect lipids.

    <p>False</p> Signup and view all the answers

    True or false: Sudan IV test is used to detect nucleic acids.

    <p>False</p> Signup and view all the answers

    True or false: Dehydration synthesis involves the addition of water to bind monomers together.

    <p>False</p> Signup and view all the answers

    True or false: Hydrolysis is a process in which an electron is removed from a molecule.

    <p>False</p> Signup and view all the answers

    True or false: Succinate being turned to fumarate is the 6th step of the Krebs cycle?

    <p>True</p> Signup and view all the answers

    True or false: The Krebs cycle takes place in the mitochondrial matrix?

    <p>True</p> Signup and view all the answers

    True or false: Glycolysis takes place in the cytoplasm?

    <p>True</p> Signup and view all the answers

    True or false: Sodium fluoride stops glycolysis?

    <p>True</p> Signup and view all the answers

    True or false: Paper chromatography utilizes the different solubility of pigments and attachment to separate the pigments from each other?

    <p>True</p> Signup and view all the answers

    True or false: Plant pigments absorb the energy from light and control photosynthesis?

    <p>True</p> Signup and view all the answers

    True or false: The equation for photosynthesis is $6CO2 + 6H2O ightarrow C6H12O6 + 6O$?

    <p>False</p> Signup and view all the answers

    True or false: The equation for cellular respiration is $C6H12O6 + 6O2 ightarrow 6CO2 + 6H2O$?

    <p>True</p> Signup and view all the answers

    What is the main difference between organic and inorganic molecules?

    <p>Organic molecules contain carbon, while inorganic molecules do not contain carbon.</p> Signup and view all the answers

    Which type of reaction involves the loss of water and the formation of a strong covalent bond between monomers?

    <p>Dehydration synthesis</p> Signup and view all the answers

    What is the main purpose of hydrolytic reactions?

    <p>To break polymers into their subunit monomers</p> Signup and view all the answers

    Which test is used to detect the presence of reducing sugars or monosaccharides?

    <p>Benedict's test</p> Signup and view all the answers

    What is the purpose of Sudan IV in the Sudan IV test for lipids?

    <p>To change color in the presence of lipids</p> Signup and view all the answers

    Which test is used to detect the presence of proteins?

    <p>Biuret test</p> Signup and view all the answers

    What are amino acids?

    <p>Monomers of proteins</p> Signup and view all the answers

    What are nucleic acids composed of?

    <p>Nucleotides</p> Signup and view all the answers

    What is the purpose of the Dische Diphenylamine test?

    <p>To distinguish DNA from RNA and other biological molecules</p> Signup and view all the answers

    What is the function of enzymes in our bodies?

    <p>To catalyze chemical reactions</p> Signup and view all the answers

    Which domain does Escherichia coli belong to?

    <p>Bacteria</p> Signup and view all the answers

    What is the function of pseudopodia in Amoeba?

    <p>Locomotion and capturing prey</p> Signup and view all the answers

    Which kingdom does Elodea belong to?

    <p>Plantae</p> Signup and view all the answers

    What is the function of the central vacuole in Elodea cells?

    <p>Holds material and wastes</p> Signup and view all the answers

    What is the function of amyloplasts in Solanum sp. (Potato) cells?

    <p>Store starch granules</p> Signup and view all the answers

    What is the main component of cell walls in Saccharomyces sp. (Yeast) cells?

    <p>Chitin</p> Signup and view all the answers

    What is the function of cilia in Paramecium?

    <p>Helps with movement</p> Signup and view all the answers

    What is visible under the microscope in human epithelium cells?

    <p>Nucleus</p> Signup and view all the answers

    What is the function of activation energy in enzymes?

    <p>Amount of energy needed for a chemical reaction</p> Signup and view all the answers

    Which domain does Paramecium belong to?

    <p>Eukarya</p> Signup and view all the answers

    Study Notes

    Scientific Method

    • Hypothesis involves making an educated guess based on prior research, often structured as "IF... THEN..."
    • Control variables are constants that allow for a fair comparison in an experiment.

    Variables in Experiments

    • Individual variable varies among subjects and can influence experimental outcomes.

    Control Groups and Sample Size

    • Control group serves as a baseline to compare results, eliminating the influence of external variables.
    • Sample size impacts the reliability of results; larger samples provide more accurate reflections of the population.

    Lipid and Protein Detection

    • Sudan IV test detects lipids, while Benedict's test identifies reducing sugars or monosaccharides.
    • Biuret test is used to detect proteins, not simple carbohydrates, which are identified by Benedict's.

    Microscopy Functions

    • Objective lens magnifies the specimen for a detailed view.
    • Iris diaphragm controls the amount of light that reaches the specimen, improving visibility.

    Calculations in Microscopy

    • Field of view (FOV) is calculated based on the diameter of the lens used.

    Enzymatic and Cellular Processes

    • Enzymes speed up biochemical reactions and lower activation energy.
    • Krebs cycle occurs in the mitochondrial matrix and is crucial for cellular respiration.

    Molecular Functions

    • Sodium fluoride inhibits glycolysis, affecting energy production.
    • Paper chromatography separates pigments based on solubility and adherence.

    Photosynthesis and Cellular Respiration Equations

    • Photosynthesis: (6CO_2 + 6H_2O \rightarrow C_6H_{12}O_6 + 6O_2)
    • Cellular respiration: (C_6H_{12}O_6 + 6O_2 \rightarrow 6CO_2 + 6H_2O)

    Organic vs. Inorganic Compounds

    • Organic molecules contain carbon and usually have covalent bonds, while inorganic molecules do not primarily consist of carbon chains.

    Chemical Reactions

    • Dehydration synthesis involves the removal of water to bond monomers, leading to the formation of larger molecules.
    • Hydrolytic reactions add water to split molecules apart.

    Plant and Microorganism Functions

    • Pseudopodia in Amoeba assist with movement and feeding.
    • Central vacuole in Elodea cells stores nutrients and maintains turgor pressure.
    • Amyloplasts in Solanum sp. (Potato) store starch.

    Cellular Structures

    • Cell walls in Saccharomyces sp. (Yeast) primarily consist of chitin.
    • Cilia in Paramecium facilitate movement.
    • Human epithelium cells display structures under microscopic examination, often including nuclei and cytoplasm.

    Nucleic Acids and Proteins

    • Nucleic acids are made of nucleotides, forming DNA or RNA.
    • Dische Diphenylamine test detects DNA through a color change.

    Classification of Organisms

    • Escherichia coli is classified under the domain Bacteria.
    • Paramecium also belongs to the domain Eukarya, as it contains a defined nucleus.

    True or False Statements

    • Controls are essential for unbiased experiments.
    • The amount of water given to plants is the independent variable.
    • Sample size directly influences research findings.
    • Carbohydrates are the first class of organic molecules.
    • Nucleic acids are organic molecules.
    • Benedict's test measures reducing sugars.
    • Hydrolysis removes electrons from a molecule (false).
    • Succinate to fumarate is not the sixth step of the Krebs cycle (false).
    • Glycolysis occurs in the cytoplasm.
    • Paper chromatography examines the solubility of pigments and their properties.
    • Plant pigments are critical for absorbing light energy in photosynthesis.

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