The Scientific Method
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Questions and Answers

Which step of the scientific method involves making an educated guess based on research?

  • Hypothesis (correct)
  • Observation
  • Experiment
  • Question
  • What is the function of control variables in an experiment?

  • To measure the dependent variable
  • To observe the control group
  • To manipulate the independent variable
  • To provide unbiased results (correct)
  • Which type of variable varies from individual to individual?

  • Independent variable
  • Dependent variable (correct)
  • Control variable
  • None of the above
  • What is the purpose of a control group in an experiment?

    <p>To provide a baseline for comparison</p> Signup and view all the answers

    Why is sample size important in an experiment?

    <p>To obtain reliable research findings</p> Signup and view all the answers

    Which test is used to detect the presence of lipids in a sample?

    <p>Sudan IV test</p> Signup and view all the answers

    What is the function of the objective lens on a microscope?

    <p>To magnify the specimen</p> Signup and view all the answers

    What is the equation for calculating the field of view (FOV) of a microscope?

    <p>FOV 10x = FOV 4x x total mag 4x</p> Signup and view all the answers

    What is the function of the iris diaphragm on a microscope?

    <p>To control the amount of light</p> Signup and view all the answers

    What is the function of enzymes in our bodies?

    <p>To speed up metabolism</p> Signup and view all the answers

    Where does the Krebs cycle take place?

    <p>Mitochondrial matrix</p> Signup and view all the answers

    What is the purpose of sodium fluoride in cellular respiration?

    <p>Inhibits glycolysis</p> Signup and view all the answers

    What is the purpose of paper chromatography?

    <p>Separating pigments</p> Signup and view all the answers

    What is the equation for photosynthesis?

    <p>6CO2 + 6H2O → C6H12O6+ 6O</p> Signup and view all the answers

    What is the equation for cellular respiration?

    <p>C6H12O6 + 6O2 → 6CO2 + 6H2O</p> Signup and view all the answers

    True or false: The hypothesis is often written using the words 'IF' and 'THEN'.

    <p>True</p> Signup and view all the answers

    True or false: Controls are essential for unbiased experiments.

    <p>True</p> Signup and view all the answers

    True or false: The amount of water given to the plants is the independent variable.

    <p>True</p> Signup and view all the answers

    True or false: Sample size directly influences research findings.

    <p>True</p> Signup and view all the answers

    True or false: Carbohydrates are the first class of organic molecules.

    <p>True</p> Signup and view all the answers

    True or false: Nucleic acids are considered organic molecules.

    <p>True</p> Signup and view all the answers

    True or false: Benedict's test is used to detect proteins.

    <p>False</p> Signup and view all the answers

    True or false: Biuret test is used to detect simple carbohydrates.

    <p>False</p> Signup and view all the answers

    True or false: Iodine test is used to detect lipids.

    <p>False</p> Signup and view all the answers

    True or false: Sudan IV test is used to detect nucleic acids.

    <p>False</p> Signup and view all the answers

    True or false: Dehydration synthesis involves the addition of water to bind monomers together.

    <p>False</p> Signup and view all the answers

    True or false: Hydrolysis is a process in which an electron is removed from a molecule.

    <p>False</p> Signup and view all the answers

    True or false: Succinate being turned to fumarate is the 6th step of the Krebs cycle?

    <p>True</p> Signup and view all the answers

    True or false: The Krebs cycle takes place in the mitochondrial matrix?

    <p>True</p> Signup and view all the answers

    True or false: Glycolysis takes place in the cytoplasm?

    <p>True</p> Signup and view all the answers

    True or false: Sodium fluoride stops glycolysis?

    <p>True</p> Signup and view all the answers

    True or false: Paper chromatography utilizes the different solubility of pigments and attachment to separate the pigments from each other?

    <p>True</p> Signup and view all the answers

    True or false: Plant pigments absorb the energy from light and control photosynthesis?

    <p>True</p> Signup and view all the answers

    True or false: The equation for photosynthesis is $6CO2 + 6H2O ightarrow C6H12O6 + 6O$?

    <p>False</p> Signup and view all the answers

    True or false: The equation for cellular respiration is $C6H12O6 + 6O2 ightarrow 6CO2 + 6H2O$?

    <p>True</p> Signup and view all the answers

    What is the main difference between organic and inorganic molecules?

    <p>Organic molecules contain carbon, while inorganic molecules do not contain carbon.</p> Signup and view all the answers

    Which type of reaction involves the loss of water and the formation of a strong covalent bond between monomers?

    <p>Dehydration synthesis</p> Signup and view all the answers

    What is the main purpose of hydrolytic reactions?

    <p>To break polymers into their subunit monomers</p> Signup and view all the answers

    Which test is used to detect the presence of reducing sugars or monosaccharides?

    <p>Benedict's test</p> Signup and view all the answers

    What is the purpose of Sudan IV in the Sudan IV test for lipids?

    <p>To change color in the presence of lipids</p> Signup and view all the answers

    Which test is used to detect the presence of proteins?

    <p>Biuret test</p> Signup and view all the answers

    What are amino acids?

    <p>Monomers of proteins</p> Signup and view all the answers

    What are nucleic acids composed of?

    <p>Nucleotides</p> Signup and view all the answers

    What is the purpose of the Dische Diphenylamine test?

    <p>To distinguish DNA from RNA and other biological molecules</p> Signup and view all the answers

    What is the function of enzymes in our bodies?

    <p>To catalyze chemical reactions</p> Signup and view all the answers

    Which domain does Escherichia coli belong to?

    <p>Bacteria</p> Signup and view all the answers

    What is the function of pseudopodia in Amoeba?

    <p>Locomotion and capturing prey</p> Signup and view all the answers

    Which kingdom does Elodea belong to?

    <p>Plantae</p> Signup and view all the answers

    What is the function of the central vacuole in Elodea cells?

    <p>Holds material and wastes</p> Signup and view all the answers

    What is the function of amyloplasts in Solanum sp. (Potato) cells?

    <p>Store starch granules</p> Signup and view all the answers

    What is the main component of cell walls in Saccharomyces sp. (Yeast) cells?

    <p>Chitin</p> Signup and view all the answers

    What is the function of cilia in Paramecium?

    <p>Helps with movement</p> Signup and view all the answers

    What is visible under the microscope in human epithelium cells?

    <p>Nucleus</p> Signup and view all the answers

    What is the function of activation energy in enzymes?

    <p>Amount of energy needed for a chemical reaction</p> Signup and view all the answers

    Which domain does Paramecium belong to?

    <p>Eukarya</p> Signup and view all the answers

    Study Notes

    Scientific Method

    • Hypothesis involves making an educated guess based on prior research, often structured as "IF... THEN..."
    • Control variables are constants that allow for a fair comparison in an experiment.

    Variables in Experiments

    • Individual variable varies among subjects and can influence experimental outcomes.

    Control Groups and Sample Size

    • Control group serves as a baseline to compare results, eliminating the influence of external variables.
    • Sample size impacts the reliability of results; larger samples provide more accurate reflections of the population.

    Lipid and Protein Detection

    • Sudan IV test detects lipids, while Benedict's test identifies reducing sugars or monosaccharides.
    • Biuret test is used to detect proteins, not simple carbohydrates, which are identified by Benedict's.

    Microscopy Functions

    • Objective lens magnifies the specimen for a detailed view.
    • Iris diaphragm controls the amount of light that reaches the specimen, improving visibility.

    Calculations in Microscopy

    • Field of view (FOV) is calculated based on the diameter of the lens used.

    Enzymatic and Cellular Processes

    • Enzymes speed up biochemical reactions and lower activation energy.
    • Krebs cycle occurs in the mitochondrial matrix and is crucial for cellular respiration.

    Molecular Functions

    • Sodium fluoride inhibits glycolysis, affecting energy production.
    • Paper chromatography separates pigments based on solubility and adherence.

    Photosynthesis and Cellular Respiration Equations

    • Photosynthesis: (6CO_2 + 6H_2O \rightarrow C_6H_{12}O_6 + 6O_2)
    • Cellular respiration: (C_6H_{12}O_6 + 6O_2 \rightarrow 6CO_2 + 6H_2O)

    Organic vs. Inorganic Compounds

    • Organic molecules contain carbon and usually have covalent bonds, while inorganic molecules do not primarily consist of carbon chains.

    Chemical Reactions

    • Dehydration synthesis involves the removal of water to bond monomers, leading to the formation of larger molecules.
    • Hydrolytic reactions add water to split molecules apart.

    Plant and Microorganism Functions

    • Pseudopodia in Amoeba assist with movement and feeding.
    • Central vacuole in Elodea cells stores nutrients and maintains turgor pressure.
    • Amyloplasts in Solanum sp. (Potato) store starch.

    Cellular Structures

    • Cell walls in Saccharomyces sp. (Yeast) primarily consist of chitin.
    • Cilia in Paramecium facilitate movement.
    • Human epithelium cells display structures under microscopic examination, often including nuclei and cytoplasm.

    Nucleic Acids and Proteins

    • Nucleic acids are made of nucleotides, forming DNA or RNA.
    • Dische Diphenylamine test detects DNA through a color change.

    Classification of Organisms

    • Escherichia coli is classified under the domain Bacteria.
    • Paramecium also belongs to the domain Eukarya, as it contains a defined nucleus.

    True or False Statements

    • Controls are essential for unbiased experiments.
    • The amount of water given to plants is the independent variable.
    • Sample size directly influences research findings.
    • Carbohydrates are the first class of organic molecules.
    • Nucleic acids are organic molecules.
    • Benedict's test measures reducing sugars.
    • Hydrolysis removes electrons from a molecule (false).
    • Succinate to fumarate is not the sixth step of the Krebs cycle (false).
    • Glycolysis occurs in the cytoplasm.
    • Paper chromatography examines the solubility of pigments and their properties.
    • Plant pigments are critical for absorbing light energy in photosynthesis.

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    Test your knowledge of the scientific method steps in order with this quiz! From making observations to forming hypotheses and conducting experiments, see if you can correctly identify each step in the scientific process.

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