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Questions and Answers
Which method is associated with traditional sequencing?
Which method is associated with traditional sequencing?
- Metagenomic Sequencing
- PCR Sequencing
- Sanger Method (correct)
- Next-Generation Sequencing
What is used in sequencing reactions along with dNTPs?
What is used in sequencing reactions along with dNTPs?
- Lipids
- RNA
- Proteins
- ddNTPs (correct)
How are fragments separated in sequencing reaction detection?
How are fragments separated in sequencing reaction detection?
- By Sequence
- By Size (correct)
- By Hydrophobicity
- By Charge
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Study Notes
Traditional Sequencing
- Sanger sequencing method is associated with traditional sequencing.
- This method uses dideoxynucleotides (ddNTPs) in sequencing reactions along with dNTPs.
- In sequencing reaction detection, fragments are separated by size using capillary electrophoresis or gel electrophoresis.
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