The Sanger DNA Sequencing Method Quiz week 4

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Questions and Answers

Which method is associated with traditional sequencing?

  • Metagenomic Sequencing
  • PCR Sequencing
  • Sanger Method (correct)
  • Next-Generation Sequencing

What is used in sequencing reactions along with dNTPs?

  • Lipids
  • RNA
  • Proteins
  • ddNTPs (correct)

How are fragments separated in sequencing reaction detection?

  • By Sequence
  • By Size (correct)
  • By Hydrophobicity
  • By Charge

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Study Notes

Traditional Sequencing

  • Sanger sequencing method is associated with traditional sequencing.
  • This method uses dideoxynucleotides (ddNTPs) in sequencing reactions along with dNTPs.
  • In sequencing reaction detection, fragments are separated by size using capillary electrophoresis or gel electrophoresis.

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