3 Questions
What are some factors that contribute to the lack of expression of differentiated function in cells grown in an invitro culture environment?
The lack of 3D scaffolding, heterogeneity, normal stimuli, and hormones found in vivo contribute to the lack of expression of differentiated function in cells grown invitro.
Why does the culture environment favor spreading, migration, and proliferation of unspecialized progenitor cells?
The culture environment favors spreading, migration, and proliferation of unspecialized progenitor cells because it lacks the 3D scaffolding and heterogeneity found in vivo, allowing these cells to move and multiply more freely.
What is the impact of the absence of normal stimuli and hormones found in vivo on cell phenotype?
The absence of normal stimuli and hormones found in vivo changes the cell microenvironment and prevents the expression of the correct phenotype in cells grown invitro.
Quiz: Understanding the Impact of In Vitro Culture on Cell Behavior and Function
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