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Questions and Answers
Who developed the Gram stain technique?
Who developed the Gram stain technique?
- Robert Koch
- Louis Pasteur
- Hans Christian Gram (correct)
- Alexander Fleming
What is the main difference between Gram-positive and Gram-negative bacteria?
What is the main difference between Gram-positive and Gram-negative bacteria?
- Gram-positive bacteria have a thicker cell membrane
- Gram-negative bacteria have a thicker peptidoglycan layer.
- Gram-positive bacteria have a thinner peptidoglycan layer. (correct)
- Gram-negative bacteria have a thicker cell wall.
What are the practical applications of the Gram stain technique?
What are the practical applications of the Gram stain technique?
- Identifying bacterial species (correct)
- Determining viral infections
- Monitoring fungal growth
- Assessing parasitic infections
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Study Notes
- The Gram stain is a widely used staining technique in microbiology.
- It was developed by Hans Christian Gram in the late 1800s.
- The stain differentiates bacterial species into Gram-positive and Gram-negative based on cell wall structure and composition.
- Gram-positive bacteria have a thicker peptidoglycan layer than Gram-negative bacteria.
- The stain works by binding to the peptidoglycan layer of the cell wall.
- Limitations of the Gram stain include its inability to distinguish between different strains of the same bacterial species or between bacteria and other microorganisms.
- Practical applications of the Gram stain include identifying bacterial species, determining antibiotic sensitivity, and monitoring treatment effectiveness.
- The Gram stain is an essential tool in microbiology for rapid and reliable bacterial identification.
- The article was written by TJ Beveridge from the University of Guelph, Ontario, Canada.
- The article provides an overview of the importance and application of the Gram stain in microbiology.
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