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Questions and Answers
What is the function of positive areas in proteins?
What is the function of positive areas in proteins?
- To make the protein more hydrophobic
- To provide catalytic functions (correct)
- To make the protein more soluble
- To make the protein more acidic
Which of the following determines whether an amino acid has a positive or negative charge?
Which of the following determines whether an amino acid has a positive or negative charge?
- The length of the amino acid chain
- The presence of hydrophobic areas
- The presence of ionizable groups (correct)
- The presence of disulfide bonds
How do the charges of charged amino acids in a polypeptide chain vary with pH?
How do the charges of charged amino acids in a polypeptide chain vary with pH?
- They remain constant
- They become more positive
- They become more negative
- They can become either more positive or more negative depending on the pH (correct)
What is the protein net charge?
What is the protein net charge?
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What is the isoelectric point of a protein?
What is the isoelectric point of a protein?
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At what pH are proteins most stable, but least soluble?
At what pH are proteins most stable, but least soluble?
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When does protein precipitation occur?
When does protein precipitation occur?
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Study Notes
- Proteins are charged in solution, with positive areas having catalytic functions and hydrophobic areas being uncharged.
- Amino acids can have positive or negative charges due to ionizable groups.
- Charged amino acids are part of a polypeptide chain and their charges vary with pH.
- The protein net charge is the sum of charges on its residues and varies with pH.
- The isoelectric point is the pH value at which the protein net charge is 0.
- Proteins are most stable at their pI but least soluble.
- Protein precipitation occurs at the pH equal to pI.
- Protein unfolding occurs at pH much higher or lower than pI.
- Ionizable groups have specific acid dissociation constants (Ka) that dictate their acidic properties.
- The pKa is the strength of an acid of an ionizable group.
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