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Questions and Answers
What is the function of positive areas in proteins?
What is the function of positive areas in proteins?
Which of the following determines whether an amino acid has a positive or negative charge?
Which of the following determines whether an amino acid has a positive or negative charge?
How do the charges of charged amino acids in a polypeptide chain vary with pH?
How do the charges of charged amino acids in a polypeptide chain vary with pH?
What is the protein net charge?
What is the protein net charge?
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What is the isoelectric point of a protein?
What is the isoelectric point of a protein?
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At what pH are proteins most stable, but least soluble?
At what pH are proteins most stable, but least soluble?
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When does protein precipitation occur?
When does protein precipitation occur?
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Study Notes
- Proteins are charged in solution, with positive areas having catalytic functions and hydrophobic areas being uncharged.
- Amino acids can have positive or negative charges due to ionizable groups.
- Charged amino acids are part of a polypeptide chain and their charges vary with pH.
- The protein net charge is the sum of charges on its residues and varies with pH.
- The isoelectric point is the pH value at which the protein net charge is 0.
- Proteins are most stable at their pI but least soluble.
- Protein precipitation occurs at the pH equal to pI.
- Protein unfolding occurs at pH much higher or lower than pI.
- Ionizable groups have specific acid dissociation constants (Ka) that dictate their acidic properties.
- The pKa is the strength of an acid of an ionizable group.
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Description
Test your knowledge on the chemistry of proteins with this quiz! From the charged areas of proteins to the isoelectric point, and the acid dissociation constants of ionizable groups, this quiz covers it all. Challenge yourself to see how much you know about the pH-dependent properties of proteins and their behavior in solution. Don't miss the chance to showcase your expertise in this fascinating topic!
