Questions and Answers
Which of the following is an advantage of HPLC?
High resolution power
In which areas is SPE widely used?
Environmental analysis
What role does light absorption play in chromatography?
It is used for detection
What is a disadvantage of coloring samples in gel electrophoresis?
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How does HPLC achieve separation?
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Study Notes
SPE and Chromatography Applications
- SPE (Solid Phase Extraction) is widely used in various fields, including environmental analysis, food testing, and forensic toxicology.
Chromatography Characteristics
- HPLC (High-Performance Liquid Chromatography) is characterized by high resolution power.
- Electrophoresis is known for its very high resolution.
Electrophoresis Fundamentals
- The negative pole at electrophoresis is the cathode.
- Coloring samples can lead to slow confirmation at gel electrophoresis.
Spectrophotometry Principles
- The proportion of light absorbed by a substance depends on the number of molecules interacting with the spectrophotometer.
HPLC Separation Mechanism
- HPLC provides separation through low pressure created by small particles in a column.
Chromatography Overview
- Chromatography is a group of methods based on the separation of components, not the absorption of light by a substance.
- Chromatography can be used to separate the components of a mixture, such as ink.
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Description
This quiz covers the applications of Solid Phase Extraction (SPE) and chromatography techniques, including HPLC and electrophoresis, in various fields such as environmental analysis and forensic toxicology.
