Chromatography and Electrophoresis Applications
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Questions and Answers

Which of the following is an advantage of HPLC?

  • High resolution power (correct)
  • Ease of use
  • Low cost
  • High sensitivity
  • In which areas is SPE widely used?

  • Environmental analysis (correct)
  • Biomedical testing
  • Geology
  • Astronomy
  • What role does light absorption play in chromatography?

  • It has no role
  • It creates high pressure in the column
  • It is used for detection (correct)
  • It separates proteins based on size
  • What is a disadvantage of coloring samples in gel electrophoresis?

    <p>Slow confirmation</p> Signup and view all the answers

    How does HPLC achieve separation?

    <p>Low pressure created by small particles in the column</p> Signup and view all the answers

    Study Notes

    SPE and Chromatography Applications

    • SPE (Solid Phase Extraction) is widely used in various fields, including environmental analysis, food testing, and forensic toxicology.

    Chromatography Characteristics

    • HPLC (High-Performance Liquid Chromatography) is characterized by high resolution power.
    • Electrophoresis is known for its very high resolution.

    Electrophoresis Fundamentals

    • The negative pole at electrophoresis is the cathode.
    • Coloring samples can lead to slow confirmation at gel electrophoresis.

    Spectrophotometry Principles

    • The proportion of light absorbed by a substance depends on the number of molecules interacting with the spectrophotometer.

    HPLC Separation Mechanism

    • HPLC provides separation through low pressure created by small particles in a column.

    Chromatography Overview

    • Chromatography is a group of methods based on the separation of components, not the absorption of light by a substance.
    • Chromatography can be used to separate the components of a mixture, such as ink.

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    Description

    This quiz covers the applications of Solid Phase Extraction (SPE) and chromatography techniques, including HPLC and electrophoresis, in various fields such as environmental analysis and forensic toxicology.

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