The FMO Enzyme Oxidation Mechanism Quiz
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Questions and Answers

What does the reactive oxygen of Flavin‐4α‐hydroperoxide attack during oxidation?

  • Hydrogen peroxide intermediate
  • A lone electron pair on a heteroatom such as Nitrogen or Sulfur (correct)
  • A typical C‐H bond
  • Enz‐FAD‐OH

    • What do steps 2 to 5 in the graph represent?

  • Formation of Enz‐FAD‐OH from Enz‐FAD‐OOH
  • Activation of hydroperoxide
  • Regeneration of Enz‐FAD‐OOH from Enz‐FAD‐OH (correct)
  • Oxidation of the substrate

    • What does FMO use as a nucleophilic displacement mechanism?

  • Hydrogen peroxide intermediate
  • Reactive oxygen of Flavin‐4α‐hydroperoxide (correct)
  • Dioxygen
  • Enz‐FAD‐OH

    • What is the state of FMO within the cell when it is ready to oxidize the substrate?

    <p>Enzyme‐bound activated hydroperoxide (Enz‐FAD‐OOH) (D)</p> Signup and view all the answers

    Which nitrogen functional groups are oxidized to form stable amine oxides?

    <p>Tertiary acyclic &amp; cyclic amines (A)</p> Signup and view all the answers

    What do secondary acyclic & cyclic amines oxidize to?

    <p>Hydroxylamines (B)</p> Signup and view all the answers

    Which nitrogen functional groups are not mentioned as being oxidized by FMO?

    <p>Primary acyclic &amp; cyclic amines (A)</p> Signup and view all the answers

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