The Clonogenic Cell Survival Assay Quiz

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Questions and Answers

Which of the following is true about cancer cells?

Cancer cells have limited growth potential
Cancer cells can grow indefinitely (correct)
Cancer cells cannot form colonies
Cancer cells can only grow in specific conditions

What is the purpose of the clonogenic cell survival assay?

To determine the ability of a cell to proliferate indefinitely
To determine the ability of a cell to form a large colony (correct)
To determine the ability of a cell to divide
To determine the ability of a cell to survive radiation

Which step is NOT part of the clonogenic cell survival assay?

Seeding at low density
Counting colonies (correct)
Culturing cancer cells
Treatment with radiation or a drug

What types of radiation are commonly used in radiation therapy?

X-rays, gamma rays, electron beams, and protons (A) Signup and view all the answers

What does radiation therapy do to cancer cells?

Destroy or damage cancer cells (A) Signup and view all the answers

What is the purpose of irradiation in the clonogenic cell survival assay?

To treat the cancer cells with energy particles or waves Signup and view all the answers

What are the steps involved in the clonogenic cell survival assay?

<ol> <li>Culturing cancer cells, 2) Treatment (e.g. irradiation or a drug), 3) Seeding at low density, 4) Colony growth over ~10 days, 5) Washing, fixation, and staining of dishes or plates when colonies reach >50 cells, 6) Counting colonies, and 7) Calculating the surviving fraction</li> </ol> Signup and view all the answers