The Clonogenic Cell Survival Assay Quiz
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Questions and Answers

Which of the following is true about cancer cells?

  • Cancer cells have limited growth potential
  • Cancer cells can grow indefinitely (correct)
  • Cancer cells cannot form colonies
  • Cancer cells can only grow in specific conditions
  • What is the purpose of the clonogenic cell survival assay?

  • To determine the ability of a cell to proliferate indefinitely
  • To determine the ability of a cell to form a large colony (correct)
  • To determine the ability of a cell to divide
  • To determine the ability of a cell to survive radiation
  • Which step is NOT part of the clonogenic cell survival assay?

  • Seeding at low density
  • Counting colonies (correct)
  • Culturing cancer cells
  • Treatment with radiation or a drug
  • What types of radiation are commonly used in radiation therapy?

    <p>X-rays, gamma rays, electron beams, and protons</p> Signup and view all the answers

    What does radiation therapy do to cancer cells?

    <p>Destroy or damage cancer cells</p> Signup and view all the answers

    What is the purpose of irradiation in the clonogenic cell survival assay?

    <p>To treat the cancer cells with energy particles or waves</p> Signup and view all the answers

    What are the steps involved in the clonogenic cell survival assay?

    <ol> <li>Culturing cancer cells, 2) Treatment (e.g. irradiation or a drug), 3) Seeding at low density, 4) Colony growth over ~10 days, 5) Washing, fixation, and staining of dishes or plates when colonies reach &gt;50 cells, 6) Counting colonies, and 7) Calculating the surviving fraction</li> </ol> Signup and view all the answers

    What is radiation therapy?

    <p>A form of anti-cancer treatment that uses high-energy particles or waves to destroy or damage cancer cells</p> Signup and view all the answers

    What does the clonogenic cell survival assay determine?

    <p>The ability of a cell to proliferate indefinitely and form a large colony</p> Signup and view all the answers

    What is radiation?

    <p>Energy emitted from a source, such as heat, light, microwaves, or X-rays</p> Signup and view all the answers

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